Characterization of a Vaginal <i>Limosilactobacillus</i> Strain Producing Anti-Virulence Postbiotics: A Potential Probiotic Candidate
The search for probiotics to help limit antibiotic resistance is a major scientific challenge. The exploration of <i>Lactobacillus</i> postbiotics represents a promising approach to prevent pathogen invasion. With this aim, <i>Limosilactobacillus fermentum</i> Lf53, with a br...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-06-01
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| Series: | Fermentation |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-5637/11/6/350 |
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| Summary: | The search for probiotics to help limit antibiotic resistance is a major scientific challenge. The exploration of <i>Lactobacillus</i> postbiotics represents a promising approach to prevent pathogen invasion. With this aim, <i>Limosilactobacillus fermentum</i> Lf53, with a broad-spectrum of antagonistic activity, was characterized as a candidate probiotic strain with promising transit tolerance and broad spectrum of activity. A study on growth and postbiotic production in modified MRS broth with different carbohydrates and its vegan variant was carried out. This study presents a comprehensive approach to characterizing the anti-virulence properties of postbiotics derived from Lf53. The promising antibacterial, antibiofilm, and anti-quorum sensing activities of the cell-free supernatants (CFS) were assessed as part of the probiotic’s barrier mechanisms. Biofilm inhibition of <i>P. aeruginosa</i> revealed remarkable suppressive effects exerted by the three tested postbiotics, two of which (nCFS and aCFS) exhibited over 50% inhibition and more than 60% for lysates. The postbiotics’ influence on the production of violacein and pyocyanin pigments of <i>Chromobacterium violaceum</i> and <i>Pseudomonas aeruginosa</i>, which are markers for quorum sensing, highlighted their potential in regulating pathogenic mechanisms. The Lf53 lysates showed the most significant inhibition of violacein production across multiple assays, showing 29.8% reduction. Regarding pyocyanin suppression, the postbiotics also demonstrated strong activity. These are the first reported data on complex postbiotics (metabiotics and parabiotics) demonstrating their potential as anti-virulence agents to help combat pathogens associated with antibiotic-resistant infections. |
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| ISSN: | 2311-5637 |