Exosome isolation and characterization for advanced diagnostic and therapeutic applications
Advancements in exosome isolation technologies are pivotal for transforming personalized medicine and enhancing clinical diagnostics. Exosomes, small extracellular vesicles with diameters ranging between 30 and 150 nm, are secreted into bodily fluids by a variety of cells and play essential roles in...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-04-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006425001711 |
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| author | Nobendu Mukerjee Arghya Bhattacharya Swastika Maitra Mandeep Kaur Subbulakshmi Ganesan Shivang Mishra Ayash Ashraf Muhammad Rizwan Kavindra Kumar Kesari Tanveer A. Tabish Nanasaheb D. Thorat |
| author_facet | Nobendu Mukerjee Arghya Bhattacharya Swastika Maitra Mandeep Kaur Subbulakshmi Ganesan Shivang Mishra Ayash Ashraf Muhammad Rizwan Kavindra Kumar Kesari Tanveer A. Tabish Nanasaheb D. Thorat |
| author_sort | Nobendu Mukerjee |
| collection | DOAJ |
| description | Advancements in exosome isolation technologies are pivotal for transforming personalized medicine and enhancing clinical diagnostics. Exosomes, small extracellular vesicles with diameters ranging between 30 and 150 nm, are secreted into bodily fluids by a variety of cells and play essential roles in intercellular communication. These vesicles facilitate the transfer of nucleic acids, lipids, and proteins, affecting a wide range of biological and pathological processes. Given their importance in disease diagnostics, therapy, and as biomarkers, there has been a surge in developing methods to isolate them from fluids such as urine, saliva, blood, and cerebrospinal fluid. While traditional isolation techniques like ultracentrifugation and polymer-based precipitation have been foundational, recent technological advances have introduced more precise methods like microfluidics and immunoaffinity capture. These newer methods enable high-throughput and specific exosome isolation by targeting surface markers, thus enhancing purity. However, challenges such as balancing purity with yield and the lack of standardized protocols across different laboratories persist, impacting the consistency of findings. By integrating advanced isolation techniques and discussing their implications in diagnostics and therapy, this review aims to catalyze further research and adoption of exosome-based technologies in medicine, marking a significant stride towards tailored healthcare solutions. |
| format | Article |
| id | doaj-art-eae03c430e48412f91f013375a04b750 |
| institution | DOAJ |
| issn | 2590-0064 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj-art-eae03c430e48412f91f013375a04b7502025-08-20T02:47:33ZengElsevierMaterials Today Bio2590-00642025-04-013110161310.1016/j.mtbio.2025.101613Exosome isolation and characterization for advanced diagnostic and therapeutic applicationsNobendu Mukerjee0Arghya Bhattacharya1Swastika Maitra2Mandeep Kaur3Subbulakshmi Ganesan4Shivang Mishra5Ayash Ashraf6Muhammad Rizwan7Kavindra Kumar Kesari8Tanveer A. Tabish9Nanasaheb D. Thorat10Centre for Infectious Diseases & Microbiology, School of Public Health Sciences and Technology, Malla Reddy Vishwavidyapeeth, Hyderabad 500 055, Telangana, IndiaDepartment of Pharmacology, Bengal School of Technology, West Bengal, Kolkata, 712102, IndiaCenter for Global Health Research, Saveetha Medical College and Hospital, Saveetha Institute of Medical and Technical Sciences, Chennai, IndiaDepartment of Sciences, Vivekananda Global University, Jaipur, Rajasthan, 303012, IndiaDepartment of Chemistry and Biochemistry, School of Sciences, JAIN (Deemed to be University), Bangalore, Karnataka, IndiaNIMS Institute of Pharmacy, NIMS University Rajasthan, Jaipur, IndiaChandigarh Pharmacy College, Chandigarh Group of College, Jhanjeri, Mohali, 140307, Punjab, IndiaDepartment of Biomedical Engineering, Department of Ophthalmology, The University of Texas Southwestern Medical Center, Dallas, TX, USADepartment of Applied Physics, Aalto University, Finland; Corresponding author.Radcliffe Department of Medicine, University of Oxford, OX3 7BN, United KingdomDepartment of Physics and Bernal Institute, University of Limerick, Castletroy, Limerick V94T9PX, Ireland; Limerick Digital Cancer Research Centre (LDCRC) University of Limerick, Castletroy, Limerick, V94T9PX, Ireland; Corresponding author. Royal Society of Medicine, United Kingdom.Advancements in exosome isolation technologies are pivotal for transforming personalized medicine and enhancing clinical diagnostics. Exosomes, small extracellular vesicles with diameters ranging between 30 and 150 nm, are secreted into bodily fluids by a variety of cells and play essential roles in intercellular communication. These vesicles facilitate the transfer of nucleic acids, lipids, and proteins, affecting a wide range of biological and pathological processes. Given their importance in disease diagnostics, therapy, and as biomarkers, there has been a surge in developing methods to isolate them from fluids such as urine, saliva, blood, and cerebrospinal fluid. While traditional isolation techniques like ultracentrifugation and polymer-based precipitation have been foundational, recent technological advances have introduced more precise methods like microfluidics and immunoaffinity capture. These newer methods enable high-throughput and specific exosome isolation by targeting surface markers, thus enhancing purity. However, challenges such as balancing purity with yield and the lack of standardized protocols across different laboratories persist, impacting the consistency of findings. By integrating advanced isolation techniques and discussing their implications in diagnostics and therapy, this review aims to catalyze further research and adoption of exosome-based technologies in medicine, marking a significant stride towards tailored healthcare solutions.http://www.sciencedirect.com/science/article/pii/S2590006425001711Exosome isolationUltracentrifugationMicrofluidicsImmunoaffinity captureExosome therapeutics |
| spellingShingle | Nobendu Mukerjee Arghya Bhattacharya Swastika Maitra Mandeep Kaur Subbulakshmi Ganesan Shivang Mishra Ayash Ashraf Muhammad Rizwan Kavindra Kumar Kesari Tanveer A. Tabish Nanasaheb D. Thorat Exosome isolation and characterization for advanced diagnostic and therapeutic applications Materials Today Bio Exosome isolation Ultracentrifugation Microfluidics Immunoaffinity capture Exosome therapeutics |
| title | Exosome isolation and characterization for advanced diagnostic and therapeutic applications |
| title_full | Exosome isolation and characterization for advanced diagnostic and therapeutic applications |
| title_fullStr | Exosome isolation and characterization for advanced diagnostic and therapeutic applications |
| title_full_unstemmed | Exosome isolation and characterization for advanced diagnostic and therapeutic applications |
| title_short | Exosome isolation and characterization for advanced diagnostic and therapeutic applications |
| title_sort | exosome isolation and characterization for advanced diagnostic and therapeutic applications |
| topic | Exosome isolation Ultracentrifugation Microfluidics Immunoaffinity capture Exosome therapeutics |
| url | http://www.sciencedirect.com/science/article/pii/S2590006425001711 |
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