THE EFFECTS OF LIQUIRITIGEN ON THE LUKEMIA INHIBITORY FACTOR RECEPTOR IN GRANULOSA CELLS OF INFERTILE PATIENTS
Objectives: Granulosa cells have an important role in oocyte nutrition and maturation, oocyte-sperm interaction events such as capacitation, and acrosome reaction. Therefore, studies with granulosa cells are also important for the healthy development of the oocyte and fertilization. The aim of this...
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Istanbul University Press
2022-10-01
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| Online Access: | https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/718E45E1D00D4C389D601D98F8C7801A |
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| author | Emel Usta Sibel Köktürk Sibel Doğan Ayşe Altun Sibel Bulgurcuoğlu Kuran Feride Özdemir |
| author_facet | Emel Usta Sibel Köktürk Sibel Doğan Ayşe Altun Sibel Bulgurcuoğlu Kuran Feride Özdemir |
| author_sort | Emel Usta |
| collection | DOAJ |
| description | Objectives: Granulosa cells have an important role in oocyte nutrition and maturation, oocyte-sperm interaction events such as capacitation, and acrosome reaction. Therefore, studies with granulosa cells are also important for the healthy development of the oocyte and fertilization. The aim of this study was to investigate the effects of liquiritigenin (LQ) in vitro on granulosa cells that were obtained from the oocyte-cumulus complex of patients who were treated in the in vitro fertilization (IVF) unit due to ovulatory factor (OF, infertile) and male factor (MF, healthy). Materials and Methods: This study included patients who were treated in the IVF unit of the Istanbul University Faculty of Medicine due to OF and MF. Granulosa cells obtained from 5 MF and 5 OF patients were divided into MF, MF-Dimethyl sulfoxide (DMSO), MF-LQ, OF, OF-DMSO and OF-LQ groups in culture medium. After the application, the cells were stained with the leukemia inhibitory factor receptor (LIFR) immunocytochemical staining and the staining intensity was examined with the Aperio ImageScope analysis software and cell morphology. Results: The LIFR immunocytochemistry staining intensity in the granulosa cells of the OF and OF-DMSO groups was significantly decreased when compared to the MF and MF-DMSO groups. The LIFR staining intensity in the granulosa cells of the MF-LQ and OF-LQ groups were determined to be significantly higher than the other groups. In the granulosa cells of OF and OF-DMSO groups, the findings reflected apoptotic cell morphology, but showed no or less apoptotic cell morphology in the MF-LQ and OF-LQ groups. Conclusions: In granulosa cells of LQ-groups, LIFR immunocytochemistry staining intensity algorithms were obtained as significantly higher than in the especially the OF and OF-DMSO groups, and showed the positive effects on apoptotic cell morphology. These results suggest that LQ may be used as an alternative medium agent in IVF units. Therefore, more comprehensive studies are needed for the clinical use of LQ. |
| format | Article |
| id | doaj-art-eabeefe668fd4e80b39e7a444c07b10e |
| institution | OA Journals |
| issn | 2651-4060 |
| language | English |
| publishDate | 2022-10-01 |
| publisher | Istanbul University Press |
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| series | Sabiad |
| spelling | doaj-art-eabeefe668fd4e80b39e7a444c07b10e2025-08-20T02:27:29ZengIstanbul University PressSabiad2651-40602022-10-015317217810.26650/JARHS2022-1129686123456THE EFFECTS OF LIQUIRITIGEN ON THE LUKEMIA INHIBITORY FACTOR RECEPTOR IN GRANULOSA CELLS OF INFERTILE PATIENTSEmel Usta0https://orcid.org/0000-0002-8549-0314Sibel Köktürk1https://orcid.org/0000-0001-5636-3300Sibel Doğan2https://orcid.org/0000-0003-4627-478XAyşe Altun3https://orcid.org/0000-0002-2765-5766Sibel Bulgurcuoğlu Kuran4https://orcid.org/0000-0003-4267-2158Feride Özdemir5https://orcid.org/0000-0003-1327-9146İstanbul Üniversitesi, İstanbul, Türkiyeİstanbul Üniversitesi, İstanbul, Türkiyeİstanbul Üniversitesi, İstanbul, Türkiyeİstanbul Üniversitesi, İstanbul, Türkiyeİstanbul Üniversitesi, İstanbul, Türkiyeİstanbul Üniversitesi, İstanbul, TürkiyeObjectives: Granulosa cells have an important role in oocyte nutrition and maturation, oocyte-sperm interaction events such as capacitation, and acrosome reaction. Therefore, studies with granulosa cells are also important for the healthy development of the oocyte and fertilization. The aim of this study was to investigate the effects of liquiritigenin (LQ) in vitro on granulosa cells that were obtained from the oocyte-cumulus complex of patients who were treated in the in vitro fertilization (IVF) unit due to ovulatory factor (OF, infertile) and male factor (MF, healthy). Materials and Methods: This study included patients who were treated in the IVF unit of the Istanbul University Faculty of Medicine due to OF and MF. Granulosa cells obtained from 5 MF and 5 OF patients were divided into MF, MF-Dimethyl sulfoxide (DMSO), MF-LQ, OF, OF-DMSO and OF-LQ groups in culture medium. After the application, the cells were stained with the leukemia inhibitory factor receptor (LIFR) immunocytochemical staining and the staining intensity was examined with the Aperio ImageScope analysis software and cell morphology. Results: The LIFR immunocytochemistry staining intensity in the granulosa cells of the OF and OF-DMSO groups was significantly decreased when compared to the MF and MF-DMSO groups. The LIFR staining intensity in the granulosa cells of the MF-LQ and OF-LQ groups were determined to be significantly higher than the other groups. In the granulosa cells of OF and OF-DMSO groups, the findings reflected apoptotic cell morphology, but showed no or less apoptotic cell morphology in the MF-LQ and OF-LQ groups. Conclusions: In granulosa cells of LQ-groups, LIFR immunocytochemistry staining intensity algorithms were obtained as significantly higher than in the especially the OF and OF-DMSO groups, and showed the positive effects on apoptotic cell morphology. These results suggest that LQ may be used as an alternative medium agent in IVF units. Therefore, more comprehensive studies are needed for the clinical use of LQ.https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/718E45E1D00D4C389D601D98F8C7801Ainfertilitycumulus-granulosa cellsleukemia inhibitory factor receptorliquiritigenin |
| spellingShingle | Emel Usta Sibel Köktürk Sibel Doğan Ayşe Altun Sibel Bulgurcuoğlu Kuran Feride Özdemir THE EFFECTS OF LIQUIRITIGEN ON THE LUKEMIA INHIBITORY FACTOR RECEPTOR IN GRANULOSA CELLS OF INFERTILE PATIENTS Sabiad infertility cumulus-granulosa cells leukemia inhibitory factor receptor liquiritigenin |
| title | THE EFFECTS OF LIQUIRITIGEN ON THE LUKEMIA INHIBITORY FACTOR RECEPTOR IN GRANULOSA CELLS OF INFERTILE PATIENTS |
| title_full | THE EFFECTS OF LIQUIRITIGEN ON THE LUKEMIA INHIBITORY FACTOR RECEPTOR IN GRANULOSA CELLS OF INFERTILE PATIENTS |
| title_fullStr | THE EFFECTS OF LIQUIRITIGEN ON THE LUKEMIA INHIBITORY FACTOR RECEPTOR IN GRANULOSA CELLS OF INFERTILE PATIENTS |
| title_full_unstemmed | THE EFFECTS OF LIQUIRITIGEN ON THE LUKEMIA INHIBITORY FACTOR RECEPTOR IN GRANULOSA CELLS OF INFERTILE PATIENTS |
| title_short | THE EFFECTS OF LIQUIRITIGEN ON THE LUKEMIA INHIBITORY FACTOR RECEPTOR IN GRANULOSA CELLS OF INFERTILE PATIENTS |
| title_sort | effects of liquiritigen on the lukemia inhibitory factor receptor in granulosa cells of infertile patients |
| topic | infertility cumulus-granulosa cells leukemia inhibitory factor receptor liquiritigenin |
| url | https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/718E45E1D00D4C389D601D98F8C7801A |
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