Polymorphisms of the collagen genes of the fibrous ring rs1800012, rs2276454, rs1793953, and the VDR gene rs2228570 with intervertebral disc degeneration

Background. Spinal diseases cause significant disability, with genetic factors influencing up to 70 % of cases. This study purposed to examine the association of polymorphisms of COL1A1rs1800012, COL2A1rs2276454, COL2A1rs1793953 (collagen genes), and VDRrs2228570 with L4-L5, L5-S1, C5-C7 with interv...

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Main Authors: Ye.H. Pedachenko, I.H. Vasylieva, M.V. Khyzhnyak, N.G. Chopyck, O.I. Tsyubko, A.B. Dmytrenko, T.A. Makarova, O.I. Troyan, T.A. Ksenzov
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Language:English
Published: Zaslavsky O.Yu. 2024-06-01
Series:Bolʹ, Sustavy, Pozvonočnik
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Online Access:https://pjs.zaslavsky.com.ua/index.php/journal/article/view/417
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author Ye.H. Pedachenko
I.H. Vasylieva
M.V. Khyzhnyak
N.G. Chopyck
O.I. Tsyubko
A.B. Dmytrenko
T.A. Makarova
O.I. Troyan
T.A. Ksenzov
author_facet Ye.H. Pedachenko
I.H. Vasylieva
M.V. Khyzhnyak
N.G. Chopyck
O.I. Tsyubko
A.B. Dmytrenko
T.A. Makarova
O.I. Troyan
T.A. Ksenzov
author_sort Ye.H. Pedachenko
collection DOAJ
description Background. Spinal diseases cause significant disability, with genetic factors influencing up to 70 % of cases. This study purposed to examine the association of polymorphisms of COL1A1rs1800012, COL2A1rs2276454, COL2A1rs1793953 (collagen genes), and VDRrs2228570 with L4-L5, L5-S1, C5-C7 with intervertebral disc degeneration among ethnic Ukrainians. Materials and methods. The study included 90 individuals with L5-S1 disc degeneration, 50 — with L4-L5 degeneration, 30 — with C5-C7, and 66 controls without disc degeneration. Applied Biosystems (USA) kits were used for genotyping. Statistical analysis was performed using SNPStats. Results. There was an association between the C/C genotype and L5-S1 disc degeneration in men (odd ratio (OR) was 2.255, 95% confidence interval (CI): 1.089–4.670; χ2 = 4.905; p = 0.027), whereas the C/T genotype may have a protective effect (OR = 0.418, 95% CІ: 0.217–0.802; χ2 = 6.689, p = 0.009). The C/T genotype may also have protective significance for C5-C7 disc degeneration in men: its occurrence was higher among men in the control group compared to women (OR = 3.85, 95% CІ: 1.086–13.648; χ2 = 4.67; p = 0.031). The G/A COL2A1rs2276454 variant may have a protective effect on the L5-S1 disc (OR = 3.50, 95% CІ: 1.26–9.72; χ2 = 6.02; p = 0.015). The pair of alleles COL2A1rs2276454/COL2A1rs1793953 were linked to degenerative changes of the L4-L5 disc in the case group (p = 0.001); COL1A1rs1800012/VDRrs2228570 and COL2A1rs1793953/VDRrs2228570 were linked to degenerative changes in the C5-C7 disc. Conclusions. The C/C VDRrs2228570 genotype in men was associated with L5-S1 intervertebral disc degeneration. The T/C VDRrs2228570 genotype may have a protective significance for men with L5-S1 and C5-C7 degeneration. The COL2A1rs2276454 variant may have a protective effect against the development of L5-S1 degenerative changes in men. The allele pairs COL1A1rs1800012/VDRrs2228570, COL2A1rs1793953/VDRrs2228570, and COL2A1rs2276454/COL2A1rs1793953 were associated with C5-C7 degeneration, while the COL2A1rs2276454/COL2A1rs1793953 pair were associated with L4-L5 degeneration.
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spelling doaj-art-eab95f3750b845cb94d88ae46327d9b32024-11-10T19:56:08ZengZaslavsky O.Yu.Bolʹ, Sustavy, Pozvonočnik2224-15072307-11332024-06-01142536010.22141/pjs.14.2.2024.417417Polymorphisms of the collagen genes of the fibrous ring rs1800012, rs2276454, rs1793953, and the VDR gene rs2228570 with intervertebral disc degenerationYe.H. Pedachenko0https://orcid.org/0000-0003-4759-6019I.H. Vasylieva1M.V. Khyzhnyak2N.G. Chopyck3https://orcid.org/0000-0003-1024-1556O.I. Tsyubko4https://orcid.org/0000-0003-0138-4643A.B. Dmytrenko5https://orcid.org/0000-0002-0141-3697T.A. Makarova6https://orcid.org/0000-0001-5203-450XO.I. Troyan7https://orcid.org/0009-0004-7122-1342T.A. Ksenzov8https://orcid.org/0000-0001-8305-8563SI “Romodanov Neurosurgery Institute of the National Academy of Medical Sciences of Ukraine”, Kyіv, UkraineSI “Romodanov Neurosurgery Institute of the National Academy of Medical Sciences of Ukraine”, Kyіv, UkraineSI “Romodanov Neurosurgery Institute of the National Academy of Medical Sciences of Ukraine”, Kyіv, UkraineSI “Romodanov Neurosurgery Institute of the National Academy of Medical Sciences of Ukraine”, Kyіv, UkraineSI “Romodanov Neurosurgery Institute of the National Academy of Medical Sciences of Ukraine”, Kyіv, UkraineSI “Romodanov Neurosurgery Institute of the National Academy of Medical Sciences of Ukraine”, Kyіv, UkraineSI “Romodanov Neurosurgery Institute of the National Academy of Medical Sciences of Ukraine”, Kyіv, UkraineBogomolets National Medical University, Kyіv, UkraineMedical House Odrex, Odesa, UkraineBackground. Spinal diseases cause significant disability, with genetic factors influencing up to 70 % of cases. This study purposed to examine the association of polymorphisms of COL1A1rs1800012, COL2A1rs2276454, COL2A1rs1793953 (collagen genes), and VDRrs2228570 with L4-L5, L5-S1, C5-C7 with intervertebral disc degeneration among ethnic Ukrainians. Materials and methods. The study included 90 individuals with L5-S1 disc degeneration, 50 — with L4-L5 degeneration, 30 — with C5-C7, and 66 controls without disc degeneration. Applied Biosystems (USA) kits were used for genotyping. Statistical analysis was performed using SNPStats. Results. There was an association between the C/C genotype and L5-S1 disc degeneration in men (odd ratio (OR) was 2.255, 95% confidence interval (CI): 1.089–4.670; χ2 = 4.905; p = 0.027), whereas the C/T genotype may have a protective effect (OR = 0.418, 95% CІ: 0.217–0.802; χ2 = 6.689, p = 0.009). The C/T genotype may also have protective significance for C5-C7 disc degeneration in men: its occurrence was higher among men in the control group compared to women (OR = 3.85, 95% CІ: 1.086–13.648; χ2 = 4.67; p = 0.031). The G/A COL2A1rs2276454 variant may have a protective effect on the L5-S1 disc (OR = 3.50, 95% CІ: 1.26–9.72; χ2 = 6.02; p = 0.015). The pair of alleles COL2A1rs2276454/COL2A1rs1793953 were linked to degenerative changes of the L4-L5 disc in the case group (p = 0.001); COL1A1rs1800012/VDRrs2228570 and COL2A1rs1793953/VDRrs2228570 were linked to degenerative changes in the C5-C7 disc. Conclusions. The C/C VDRrs2228570 genotype in men was associated with L5-S1 intervertebral disc degeneration. The T/C VDRrs2228570 genotype may have a protective significance for men with L5-S1 and C5-C7 degeneration. The COL2A1rs2276454 variant may have a protective effect against the development of L5-S1 degenerative changes in men. The allele pairs COL1A1rs1800012/VDRrs2228570, COL2A1rs1793953/VDRrs2228570, and COL2A1rs2276454/COL2A1rs1793953 were associated with C5-C7 degeneration, while the COL2A1rs2276454/COL2A1rs1793953 pair were associated with L4-L5 degeneration.https://pjs.zaslavsky.com.ua/index.php/journal/article/view/417intervertebral disc degenerationrs1800012rs2276454rs1793953rs2228570linkage disequilibrium
spellingShingle Ye.H. Pedachenko
I.H. Vasylieva
M.V. Khyzhnyak
N.G. Chopyck
O.I. Tsyubko
A.B. Dmytrenko
T.A. Makarova
O.I. Troyan
T.A. Ksenzov
Polymorphisms of the collagen genes of the fibrous ring rs1800012, rs2276454, rs1793953, and the VDR gene rs2228570 with intervertebral disc degeneration
Bolʹ, Sustavy, Pozvonočnik
intervertebral disc degeneration
rs1800012
rs2276454
rs1793953
rs2228570
linkage disequilibrium
title Polymorphisms of the collagen genes of the fibrous ring rs1800012, rs2276454, rs1793953, and the VDR gene rs2228570 with intervertebral disc degeneration
title_full Polymorphisms of the collagen genes of the fibrous ring rs1800012, rs2276454, rs1793953, and the VDR gene rs2228570 with intervertebral disc degeneration
title_fullStr Polymorphisms of the collagen genes of the fibrous ring rs1800012, rs2276454, rs1793953, and the VDR gene rs2228570 with intervertebral disc degeneration
title_full_unstemmed Polymorphisms of the collagen genes of the fibrous ring rs1800012, rs2276454, rs1793953, and the VDR gene rs2228570 with intervertebral disc degeneration
title_short Polymorphisms of the collagen genes of the fibrous ring rs1800012, rs2276454, rs1793953, and the VDR gene rs2228570 with intervertebral disc degeneration
title_sort polymorphisms of the collagen genes of the fibrous ring rs1800012 rs2276454 rs1793953 and the vdr gene rs2228570 with intervertebral disc degeneration
topic intervertebral disc degeneration
rs1800012
rs2276454
rs1793953
rs2228570
linkage disequilibrium
url https://pjs.zaslavsky.com.ua/index.php/journal/article/view/417
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AT ihvasylieva polymorphismsofthecollagengenesofthefibrousringrs1800012rs2276454rs1793953andthevdrgeners2228570withintervertebraldiscdegeneration
AT mvkhyzhnyak polymorphismsofthecollagengenesofthefibrousringrs1800012rs2276454rs1793953andthevdrgeners2228570withintervertebraldiscdegeneration
AT ngchopyck polymorphismsofthecollagengenesofthefibrousringrs1800012rs2276454rs1793953andthevdrgeners2228570withintervertebraldiscdegeneration
AT oitsyubko polymorphismsofthecollagengenesofthefibrousringrs1800012rs2276454rs1793953andthevdrgeners2228570withintervertebraldiscdegeneration
AT abdmytrenko polymorphismsofthecollagengenesofthefibrousringrs1800012rs2276454rs1793953andthevdrgeners2228570withintervertebraldiscdegeneration
AT tamakarova polymorphismsofthecollagengenesofthefibrousringrs1800012rs2276454rs1793953andthevdrgeners2228570withintervertebraldiscdegeneration
AT oitroyan polymorphismsofthecollagengenesofthefibrousringrs1800012rs2276454rs1793953andthevdrgeners2228570withintervertebraldiscdegeneration
AT taksenzov polymorphismsofthecollagengenesofthefibrousringrs1800012rs2276454rs1793953andthevdrgeners2228570withintervertebraldiscdegeneration