Two Kinds of Proteases Improved the Water-holding Capacity and Quality of Phosphorus-reduced Steak

In this study, the effects of papain and glutamine transaminase on the water-holding capacity and quality of phosphorus-reduced steaks were investigated. Fresh bovine outer spine meat was studied in a one-way test using water-holding capacity, low-field nuclear magnetic resonance relaxation time, sc...

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Main Authors: Xiaojuan JIANG, Deyin PAN, Bing LI, Hongsheng CHEN, Jingjing DIAO
Format: Article
Language:zho
Published: The editorial department of Science and Technology of Food Industry 2024-12-01
Series:Shipin gongye ke-ji
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Online Access:http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023120319
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author Xiaojuan JIANG
Deyin PAN
Bing LI
Hongsheng CHEN
Jingjing DIAO
author_facet Xiaojuan JIANG
Deyin PAN
Bing LI
Hongsheng CHEN
Jingjing DIAO
author_sort Xiaojuan JIANG
collection DOAJ
description In this study, the effects of papain and glutamine transaminase on the water-holding capacity and quality of phosphorus-reduced steaks were investigated. Fresh bovine outer spine meat was studied in a one-way test using water-holding capacity, low-field nuclear magnetic resonance relaxation time, scanning electron microscopy, and shear force as evaluation indices. Effects on the water-holding capacity, processing quality, and organoleptic quality of steaks were compared for the control group (non-additive), phosphate group (phosphate additions of 0.2% and 0.4%), papain phosphorus-reduced group (0.2% phosphate with concentrations of 0.03%, 0.05% and 0.07% papain), and glutamine aminotransferase phosphorus-reduced group (0.2% phosphate with concentrations of 0.4%, 0.6%, and 0.8% glutamine aminotransferase). The results showed that the addition of 0.05% papain significantly reduced (P<0.05) steak cooking loss by 4.89% and shear force by 25.67 N and improved steak organoleptic quality when compared to the effects for the 0.2% phosphate group, and there were no significant difference (P>0.05) compared with the 0.4% phosphate group. Therefore, use of this treatment could effectively replace 50% of the amount of phosphate in the steak. In the glutamine aminotransferase phosphorus-reduced group, both cooking loss and shear force gradually decreased with an increase in the addition of glutamine aminotransferase, and no significant improvement (P>0.05) in product quality was noted when the added amount was greater than 0.6%. In conclusion, papain treatment at a concentration of 0.05% could effectively replace the addition of certain phosphates during meat processing and provide new ideas for the production of green, clean, and phosphorus-reduced meat products.
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spelling doaj-art-eab7e1bd874040699c2e0f848144aa192025-08-20T02:36:03ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062024-12-014524596710.13386/j.issn1002-0306.20231203192023120319-24Two Kinds of Proteases Improved the Water-holding Capacity and Quality of Phosphorus-reduced SteakXiaojuan JIANG0Deyin PAN1Bing LI2Hongsheng CHEN3Jingjing DIAO4College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, ChinaCollege of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, ChinaCollege of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, ChinaCollege of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, ChinaCollege of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, ChinaIn this study, the effects of papain and glutamine transaminase on the water-holding capacity and quality of phosphorus-reduced steaks were investigated. Fresh bovine outer spine meat was studied in a one-way test using water-holding capacity, low-field nuclear magnetic resonance relaxation time, scanning electron microscopy, and shear force as evaluation indices. Effects on the water-holding capacity, processing quality, and organoleptic quality of steaks were compared for the control group (non-additive), phosphate group (phosphate additions of 0.2% and 0.4%), papain phosphorus-reduced group (0.2% phosphate with concentrations of 0.03%, 0.05% and 0.07% papain), and glutamine aminotransferase phosphorus-reduced group (0.2% phosphate with concentrations of 0.4%, 0.6%, and 0.8% glutamine aminotransferase). The results showed that the addition of 0.05% papain significantly reduced (P<0.05) steak cooking loss by 4.89% and shear force by 25.67 N and improved steak organoleptic quality when compared to the effects for the 0.2% phosphate group, and there were no significant difference (P>0.05) compared with the 0.4% phosphate group. Therefore, use of this treatment could effectively replace 50% of the amount of phosphate in the steak. In the glutamine aminotransferase phosphorus-reduced group, both cooking loss and shear force gradually decreased with an increase in the addition of glutamine aminotransferase, and no significant improvement (P>0.05) in product quality was noted when the added amount was greater than 0.6%. In conclusion, papain treatment at a concentration of 0.05% could effectively replace the addition of certain phosphates during meat processing and provide new ideas for the production of green, clean, and phosphorus-reduced meat products.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023120319steakphosphorus reductionwater-holding capacityphosphate complexpapain
spellingShingle Xiaojuan JIANG
Deyin PAN
Bing LI
Hongsheng CHEN
Jingjing DIAO
Two Kinds of Proteases Improved the Water-holding Capacity and Quality of Phosphorus-reduced Steak
Shipin gongye ke-ji
steak
phosphorus reduction
water-holding capacity
phosphate complex
papain
title Two Kinds of Proteases Improved the Water-holding Capacity and Quality of Phosphorus-reduced Steak
title_full Two Kinds of Proteases Improved the Water-holding Capacity and Quality of Phosphorus-reduced Steak
title_fullStr Two Kinds of Proteases Improved the Water-holding Capacity and Quality of Phosphorus-reduced Steak
title_full_unstemmed Two Kinds of Proteases Improved the Water-holding Capacity and Quality of Phosphorus-reduced Steak
title_short Two Kinds of Proteases Improved the Water-holding Capacity and Quality of Phosphorus-reduced Steak
title_sort two kinds of proteases improved the water holding capacity and quality of phosphorus reduced steak
topic steak
phosphorus reduction
water-holding capacity
phosphate complex
papain
url http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023120319
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AT bingli twokindsofproteasesimprovedthewaterholdingcapacityandqualityofphosphorusreducedsteak
AT hongshengchen twokindsofproteasesimprovedthewaterholdingcapacityandqualityofphosphorusreducedsteak
AT jingjingdiao twokindsofproteasesimprovedthewaterholdingcapacityandqualityofphosphorusreducedsteak