Pollen–Pistil Interactions in Autochthonous Balkan Sweet Cherry Cultivars—The Impact of Genotype and Flowering Temperature
The efficacy of sweet cherry production is highly dependent on the regularity of flowering events and genetic-determined relations between female sporophyte and male gametophyte, which became even more important with higher flowering temperatures caused by climate change. Special attention is paid t...
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| Format: | Article |
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MDPI AG
2025-03-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/15/3/646 |
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| author | Sanja Radičević Slađana Marić Ivana Glišić Radosav Cerović Milena Đorđević Nebojša Milošević Vera Rakonjac Slavica Čolić Melpomena Popovska Viktor Gjamovski Bojana Banović Đeri |
| author_facet | Sanja Radičević Slađana Marić Ivana Glišić Radosav Cerović Milena Đorđević Nebojša Milošević Vera Rakonjac Slavica Čolić Melpomena Popovska Viktor Gjamovski Bojana Banović Đeri |
| author_sort | Sanja Radičević |
| collection | DOAJ |
| description | The efficacy of sweet cherry production is highly dependent on the regularity of flowering events and genetic-determined relations between female sporophyte and male gametophyte, which became even more important with higher flowering temperatures caused by climate change. Special attention is paid to the genetic diversity that provides essential sources of potential temperature-tolerance genes. Our study aimed at the genetic and reproductive characterization of Balkan cherry cultivars of autochthonous origin (‘Canetova’, ‘G-2’, ‘Dolga Šiška’ and ‘Ohridska Crna’), and six potential pollenizers. To identify <i>S</i>-haplotypes, the polymerase chain reaction (PCR) method was used to detect the <i>S</i>-ribonuclease (<i>S-RNase</i>) and <i>S</i>-haplotype-specific F-box protein (<i>SFB</i>) alleles, combined with fragment analysis and <i>S-RNase</i> sequencing. Pollination experiments were performed at three Balkan localities over two flowering seasons, and the fluorescence microscopy method was used to assess the cultivars’ male/female reproductive behaviour. A novel <i>S-RNase</i> allele <i>S<sub>40</sub></i> was identified in ‘Ohridska Crna’ for the first time. ‘Ohridska Crna’ also demonstrated the best adaptability to higher temperatures regarding primary ovule longevity. This feature makes it desirable from the aspect of breeding new cultivars that can withstand the impacts of climate change. The findings on male-female relations and their temperature dependence open up the possibility for yield prediction and smart horticultural decisions that can be made to guide cherry production. |
| format | Article |
| id | doaj-art-eaa7dd8fc97e4f278807f34fbca651fb |
| institution | Kabale University |
| issn | 2073-4395 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj-art-eaa7dd8fc97e4f278807f34fbca651fb2025-08-20T03:40:42ZengMDPI AGAgronomy2073-43952025-03-0115364610.3390/agronomy15030646Pollen–Pistil Interactions in Autochthonous Balkan Sweet Cherry Cultivars—The Impact of Genotype and Flowering TemperatureSanja Radičević0Slađana Marić1Ivana Glišić2Radosav Cerović3Milena Đorđević4Nebojša Milošević5Vera Rakonjac6Slavica Čolić7Melpomena Popovska8Viktor Gjamovski9Bojana Banović Đeri10Fruit Research Institute, Čačak, 32000 Čačak, SerbiaFruit Research Institute, Čačak, 32000 Čačak, SerbiaFruit Research Institute, Čačak, 32000 Čačak, SerbiaInnovation Center of Faculty of Technology and Metallurgy, University of Belgrade, 11120 Belgrade, SerbiaFruit Research Institute, Čačak, 32000 Čačak, SerbiaFruit Research Institute, Čačak, 32000 Čačak, SerbiaFaculty of Agriculture, University of Belgrade, 11080 Belgrade, SerbiaInstitute for Science Application in Agriculture, 11000 Belgrade, SerbiaInstitute of Agriculture, Ss. Cyril and Methodius University in Skopje, 1000 Skopje, North MacedoniaInstitute of Agriculture, Ss. Cyril and Methodius University in Skopje, 1000 Skopje, North MacedoniaInstitute of Molecular Genetics and Genetic Engineering, University of Belgrade, 11042 Belgrade, SerbiaThe efficacy of sweet cherry production is highly dependent on the regularity of flowering events and genetic-determined relations between female sporophyte and male gametophyte, which became even more important with higher flowering temperatures caused by climate change. Special attention is paid to the genetic diversity that provides essential sources of potential temperature-tolerance genes. Our study aimed at the genetic and reproductive characterization of Balkan cherry cultivars of autochthonous origin (‘Canetova’, ‘G-2’, ‘Dolga Šiška’ and ‘Ohridska Crna’), and six potential pollenizers. To identify <i>S</i>-haplotypes, the polymerase chain reaction (PCR) method was used to detect the <i>S</i>-ribonuclease (<i>S-RNase</i>) and <i>S</i>-haplotype-specific F-box protein (<i>SFB</i>) alleles, combined with fragment analysis and <i>S-RNase</i> sequencing. Pollination experiments were performed at three Balkan localities over two flowering seasons, and the fluorescence microscopy method was used to assess the cultivars’ male/female reproductive behaviour. A novel <i>S-RNase</i> allele <i>S<sub>40</sub></i> was identified in ‘Ohridska Crna’ for the first time. ‘Ohridska Crna’ also demonstrated the best adaptability to higher temperatures regarding primary ovule longevity. This feature makes it desirable from the aspect of breeding new cultivars that can withstand the impacts of climate change. The findings on male-female relations and their temperature dependence open up the possibility for yield prediction and smart horticultural decisions that can be made to guide cherry production.https://www.mdpi.com/2073-4395/15/3/646<i>Prunus avium</i> L.landraces<i>S</i>-allelesself-(in)compatibilityflowering temperaturespollen tube growth |
| spellingShingle | Sanja Radičević Slađana Marić Ivana Glišić Radosav Cerović Milena Đorđević Nebojša Milošević Vera Rakonjac Slavica Čolić Melpomena Popovska Viktor Gjamovski Bojana Banović Đeri Pollen–Pistil Interactions in Autochthonous Balkan Sweet Cherry Cultivars—The Impact of Genotype and Flowering Temperature Agronomy <i>Prunus avium</i> L. landraces <i>S</i>-alleles self-(in)compatibility flowering temperatures pollen tube growth |
| title | Pollen–Pistil Interactions in Autochthonous Balkan Sweet Cherry Cultivars—The Impact of Genotype and Flowering Temperature |
| title_full | Pollen–Pistil Interactions in Autochthonous Balkan Sweet Cherry Cultivars—The Impact of Genotype and Flowering Temperature |
| title_fullStr | Pollen–Pistil Interactions in Autochthonous Balkan Sweet Cherry Cultivars—The Impact of Genotype and Flowering Temperature |
| title_full_unstemmed | Pollen–Pistil Interactions in Autochthonous Balkan Sweet Cherry Cultivars—The Impact of Genotype and Flowering Temperature |
| title_short | Pollen–Pistil Interactions in Autochthonous Balkan Sweet Cherry Cultivars—The Impact of Genotype and Flowering Temperature |
| title_sort | pollen pistil interactions in autochthonous balkan sweet cherry cultivars the impact of genotype and flowering temperature |
| topic | <i>Prunus avium</i> L. landraces <i>S</i>-alleles self-(in)compatibility flowering temperatures pollen tube growth |
| url | https://www.mdpi.com/2073-4395/15/3/646 |
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