Effects of Qinggan-27 Recipe on Nonalcoholic Steatohepatitis Induced by High-fat Diet in Rats

Objective: The Qinggan-27 (QG-27) recipe is a traditional Mongolian medicine believed to improve liver function during Non-alcoholic steatohepatitis (NASH). We developed fatty liver models in rats by intragastric administration of a high-fat diet (HFD) to investigate the effects of the QG-27 recipe...

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Main Authors: WenJun, Khaliun Erdenebat, Tserentsoo Byambaa, Bayarmaa Enkhbat, Enkhtuguldur Myagmar-Ochir, Sayamaa Lkhagvadorj
Format: Article
Language:English
Published: Mongolian National University of Medical Sciences 2024-09-01
Series:Central Asian Journal of Medical Sciences
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Online Access:https://www.mongoliajol.info/index.php/CAJMS/article/view/3949
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author WenJun
Khaliun Erdenebat
Tserentsoo Byambaa
Bayarmaa Enkhbat
Enkhtuguldur Myagmar-Ochir
Sayamaa Lkhagvadorj
author_facet WenJun
Khaliun Erdenebat
Tserentsoo Byambaa
Bayarmaa Enkhbat
Enkhtuguldur Myagmar-Ochir
Sayamaa Lkhagvadorj
author_sort WenJun
collection DOAJ
description Objective: The Qinggan-27 (QG-27) recipe is a traditional Mongolian medicine believed to improve liver function during Non-alcoholic steatohepatitis (NASH). We developed fatty liver models in rats by intragastric administration of a high-fat diet (HFD) to investigate the effects of the QG-27 recipe on the NASH model through the expression of PGC-1 α and UCP-2. Methods: Wister rats were fed with specially prepared HFD to create a pathological model for 6 weeks. After successful modeling, the patient was treated with QG-27 twice daily with intra­gastric administration of QG-27 at 150 mg/kg, 300 mg/kg, and 600 mg/kg, respectively, for 21 days. Biochemical, histopathological, and immunohistochemical analyses were conducted to evaluate the effect of QG-27 treatment. Results: Levels of AST, ALT, LDL-C, TG, and TC were significantly decreased, and HDL-C was increased in the intermediate dose of the QG-27 group compared to the control group (p<0.05). Immunohistochemical results demonstrated that an intermediate dose of the QG-27 recipe could significantly improve liver function in rats with fat­ty liver by increasing PGC-1α and UCP-2 expression. Conclusion: The intermediate (300 mg/ kg) dose of the QG-27 is a good candidate to be a dietary supplement for reducing fatty liver.
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institution Kabale University
issn 2413-8681
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language English
publishDate 2024-09-01
publisher Mongolian National University of Medical Sciences
record_format Article
series Central Asian Journal of Medical Sciences
spelling doaj-art-eaa3209bb3f042ba8535660895ec23de2025-08-20T03:25:26ZengMongolian National University of Medical SciencesCentral Asian Journal of Medical Sciences2413-86812414-97722024-09-01103859410.24079/cajms.2024.03.0013900Effects of Qinggan-27 Recipe on Nonalcoholic Steatohepatitis Induced by High-fat Diet in RatsWenJun0Khaliun Erdenebat1Tserentsoo Byambaa2Bayarmaa Enkhbat3Enkhtuguldur Myagmar-Ochir4Sayamaa Lkhagvadorj5https://orcid.org/0000-0001-6924-8715Department of Traditional Prescriptionology, International School of Mongolian Medicine, Mongolian National University of Medical Sciences, Ulaanbaatar, MongoliaDepartment of Pathology and Forensic Medicine, School of Biomedicine, Mongolian National University of Medical Sciences, Ulaanbaatar, MongoliaDepartment of Traditional Prescriptionology, International School of Mongolian Medicine, Mongolian National University of Medical Sciences, Ulaanbaatar, MongoliaDepartment of Pathology and Forensic Medicine, School of Biomedicine, Mongolian National University of Medical Sciences, Ulaanbaatar, MongoliaDepartment of epidemiology and biostatistics, School of Public Health, Mongolian National University of Medical Sciences, Ulaanbaatar, MongoliaDepartment of Pathology and Forensic Medicine, School of Biomedicine, Mongolian National University of Medical Sciences, Ulaanbaatar, MongoliaObjective: The Qinggan-27 (QG-27) recipe is a traditional Mongolian medicine believed to improve liver function during Non-alcoholic steatohepatitis (NASH). We developed fatty liver models in rats by intragastric administration of a high-fat diet (HFD) to investigate the effects of the QG-27 recipe on the NASH model through the expression of PGC-1 α and UCP-2. Methods: Wister rats were fed with specially prepared HFD to create a pathological model for 6 weeks. After successful modeling, the patient was treated with QG-27 twice daily with intra­gastric administration of QG-27 at 150 mg/kg, 300 mg/kg, and 600 mg/kg, respectively, for 21 days. Biochemical, histopathological, and immunohistochemical analyses were conducted to evaluate the effect of QG-27 treatment. Results: Levels of AST, ALT, LDL-C, TG, and TC were significantly decreased, and HDL-C was increased in the intermediate dose of the QG-27 group compared to the control group (p<0.05). Immunohistochemical results demonstrated that an intermediate dose of the QG-27 recipe could significantly improve liver function in rats with fat­ty liver by increasing PGC-1α and UCP-2 expression. Conclusion: The intermediate (300 mg/ kg) dose of the QG-27 is a good candidate to be a dietary supplement for reducing fatty liver.https://www.mongoliajol.info/index.php/CAJMS/article/view/3949disease modelnon-alcoholic fatty livermongolian medicinepgc-1αucp-2
spellingShingle WenJun
Khaliun Erdenebat
Tserentsoo Byambaa
Bayarmaa Enkhbat
Enkhtuguldur Myagmar-Ochir
Sayamaa Lkhagvadorj
Effects of Qinggan-27 Recipe on Nonalcoholic Steatohepatitis Induced by High-fat Diet in Rats
Central Asian Journal of Medical Sciences
disease model
non-alcoholic fatty liver
mongolian medicine
pgc-1α
ucp-2
title Effects of Qinggan-27 Recipe on Nonalcoholic Steatohepatitis Induced by High-fat Diet in Rats
title_full Effects of Qinggan-27 Recipe on Nonalcoholic Steatohepatitis Induced by High-fat Diet in Rats
title_fullStr Effects of Qinggan-27 Recipe on Nonalcoholic Steatohepatitis Induced by High-fat Diet in Rats
title_full_unstemmed Effects of Qinggan-27 Recipe on Nonalcoholic Steatohepatitis Induced by High-fat Diet in Rats
title_short Effects of Qinggan-27 Recipe on Nonalcoholic Steatohepatitis Induced by High-fat Diet in Rats
title_sort effects of qinggan 27 recipe on nonalcoholic steatohepatitis induced by high fat diet in rats
topic disease model
non-alcoholic fatty liver
mongolian medicine
pgc-1α
ucp-2
url https://www.mongoliajol.info/index.php/CAJMS/article/view/3949
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