Molecular epidemiology of hepatitis B virus from blood samples obtained from persons in Enugu and Nasarawa States, Nigeria
Abstract Hepatitis B virus (HBV) infection remains a major global health concern, with a high burden in countries like Nigeria. This study aimed to investigate the molecular epidemiology of HBV by identifying circulating genotypes and detecting antiviral-resistant mutations in samples from selected...
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BMC
2025-05-01
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| Series: | BMC Infectious Diseases |
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| Online Access: | https://doi.org/10.1186/s12879-025-11166-0 |
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| author | Kingsley Andrew Egbe Anthony C. Ike Olusola Anuoluwapo Akanbi Richard Olulowo Ojedele Khadijah Isa Imam Chimaobi Chukwu Friday Egbe Edith Silas Ekwom Nse F. Unam Ikeata Kenneth Chukwuemeka |
| author_facet | Kingsley Andrew Egbe Anthony C. Ike Olusola Anuoluwapo Akanbi Richard Olulowo Ojedele Khadijah Isa Imam Chimaobi Chukwu Friday Egbe Edith Silas Ekwom Nse F. Unam Ikeata Kenneth Chukwuemeka |
| author_sort | Kingsley Andrew Egbe |
| collection | DOAJ |
| description | Abstract Hepatitis B virus (HBV) infection remains a major global health concern, with a high burden in countries like Nigeria. This study aimed to investigate the molecular epidemiology of HBV by identifying circulating genotypes and detecting antiviral-resistant mutations in samples from selected communities in Enugu and Nasarawa States, Nigeria. Sample (plasma and serum) quality was evaluated after prolonged storage under freeze-thaw conditions using Qubit 4 fluorometer and Nanodrop One spectrophotometer. High-quality samples underwent molecular characterization and Sanger sequencing for HBV-positive cases. Of the 67 samples analyzed, seven were sequenced: two from Enugu and four from Nasarawa belonged to genotype E, while one from Nasarawa was genotype A. No antiviral resistance mutations were identified. This study provides valuable molecular data contributing to our understanding of HBV epidemiology in Nigeria. |
| format | Article |
| id | doaj-art-ea953e962f304164beec6454d3005eb7 |
| institution | DOAJ |
| issn | 1471-2334 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj-art-ea953e962f304164beec6454d3005eb72025-08-20T03:22:11ZengBMCBMC Infectious Diseases1471-23342025-05-012511710.1186/s12879-025-11166-0Molecular epidemiology of hepatitis B virus from blood samples obtained from persons in Enugu and Nasarawa States, NigeriaKingsley Andrew Egbe0Anthony C. Ike1Olusola Anuoluwapo Akanbi2Richard Olulowo Ojedele3Khadijah Isa Imam4Chimaobi Chukwu5Friday Egbe6Edith Silas Ekwom7Nse F. Unam8Ikeata Kenneth Chukwuemeka9Department of Microbiology, Faculty of Biological Sciences, University of Nigeria NsukkaDepartment of Microbiology, Faculty of Biological Sciences, University of Nigeria NsukkaGenomics Laboratory, Nigerian Centre for Disease Control, National Reference LaboratoryGenomics Laboratory, Nigerian Centre for Disease Control, National Reference LaboratoryGenomics Laboratory, Nigerian Centre for Disease Control, National Reference LaboratoryGenomics Laboratory, Nigerian Centre for Disease Control, National Reference LaboratoryPublic Health Department, Federal Capital Territory AdministrationDepartment of Microbiology, Faculty of Science, Cross River University of TechnologyHaematology Unit, Federal Staff Clinic GwarinpaGenomics Laboratory, Nigerian Centre for Disease Control, National Reference LaboratoryAbstract Hepatitis B virus (HBV) infection remains a major global health concern, with a high burden in countries like Nigeria. This study aimed to investigate the molecular epidemiology of HBV by identifying circulating genotypes and detecting antiviral-resistant mutations in samples from selected communities in Enugu and Nasarawa States, Nigeria. Sample (plasma and serum) quality was evaluated after prolonged storage under freeze-thaw conditions using Qubit 4 fluorometer and Nanodrop One spectrophotometer. High-quality samples underwent molecular characterization and Sanger sequencing for HBV-positive cases. Of the 67 samples analyzed, seven were sequenced: two from Enugu and four from Nasarawa belonged to genotype E, while one from Nasarawa was genotype A. No antiviral resistance mutations were identified. This study provides valuable molecular data contributing to our understanding of HBV epidemiology in Nigeria.https://doi.org/10.1186/s12879-025-11166-0Hepatitis B virusGenotypeAntiviral resistanceMolecular epidemiologyEnugu stateNasarawa state |
| spellingShingle | Kingsley Andrew Egbe Anthony C. Ike Olusola Anuoluwapo Akanbi Richard Olulowo Ojedele Khadijah Isa Imam Chimaobi Chukwu Friday Egbe Edith Silas Ekwom Nse F. Unam Ikeata Kenneth Chukwuemeka Molecular epidemiology of hepatitis B virus from blood samples obtained from persons in Enugu and Nasarawa States, Nigeria BMC Infectious Diseases Hepatitis B virus Genotype Antiviral resistance Molecular epidemiology Enugu state Nasarawa state |
| title | Molecular epidemiology of hepatitis B virus from blood samples obtained from persons in Enugu and Nasarawa States, Nigeria |
| title_full | Molecular epidemiology of hepatitis B virus from blood samples obtained from persons in Enugu and Nasarawa States, Nigeria |
| title_fullStr | Molecular epidemiology of hepatitis B virus from blood samples obtained from persons in Enugu and Nasarawa States, Nigeria |
| title_full_unstemmed | Molecular epidemiology of hepatitis B virus from blood samples obtained from persons in Enugu and Nasarawa States, Nigeria |
| title_short | Molecular epidemiology of hepatitis B virus from blood samples obtained from persons in Enugu and Nasarawa States, Nigeria |
| title_sort | molecular epidemiology of hepatitis b virus from blood samples obtained from persons in enugu and nasarawa states nigeria |
| topic | Hepatitis B virus Genotype Antiviral resistance Molecular epidemiology Enugu state Nasarawa state |
| url | https://doi.org/10.1186/s12879-025-11166-0 |
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