High frequency of HPV high-risk preventable genotypes in Ecuadorian women with invasive cervical cancer
Objective. To determine the distribution of human papillomavirus (HPV) genotypes in invasive cervical cancer samples from Ecuadorian women who attended the Cancer Institute (Sociedad de Lucha Contra el Cáncer – SOLCA). Methods. Archived formalin-fixed paraffin-embedded (FFPE) cervical cancer tissue...
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| Format: | Article |
| Language: | English |
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Pan American Health Organization
2024-12-01
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| Series: | Revista Panamericana de Salud Pública |
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| Online Access: | https://iris.paho.org/handle/10665.2/62660 |
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| author | Gustavo David García Muentes Fadi Abdul Latif Claudia Catalina Beltrán Rodríguez Sebastián Medina González Juan Carlos Ruiz Cabezas |
| author_facet | Gustavo David García Muentes Fadi Abdul Latif Claudia Catalina Beltrán Rodríguez Sebastián Medina González Juan Carlos Ruiz Cabezas |
| author_sort | Gustavo David García Muentes |
| collection | DOAJ |
| description | Objective. To determine the distribution of human papillomavirus (HPV) genotypes in invasive cervical cancer samples from Ecuadorian women who attended the Cancer Institute (Sociedad de Lucha Contra el Cáncer – SOLCA).
Methods. Archived formalin-fixed paraffin-embedded (FFPE) cervical cancer tissue samples collected during 2017–2021 were deparaffinized, and nucleic acid extraction and purification was performed using silica columns. The obtained nucleic acids were analyzed using INNO-LiPA® HPV Genotyping Extra II per the manufacturer’s specifications. Data were retrieved from records, and HPV genotypes were determined from the FFPE samples.
Results. The study included samples from 190 women diagnosed with invasive cervical cancer, with a median age of 52.78 years. Squamous cell carcinoma accounted for 78.94% of the cases, while 21.05% had adenocarcinoma. Among the 190 samples, 80.53% tested positive for HPV DNA, while 19.47% were negative. The most common genotypes detected were HPV 16 (64.05%), 18 (16.99%), and 58 (6.54%). HPV infection frequency was higher in samples from patients with elementary level education (p < 0.05).
Conclusions. This study provides valuable insights into the distribution of HPV genotypes in invasive cervical cancer samples from Ecuadorian women. The results indicate an elevated presence of HPV 16, HPV 18, and HPV 58, which are vaccine-preventable genotypes. |
| format | Article |
| id | doaj-art-ea91b5d7bc6d4bae97bac053c7cdd9fa |
| institution | OA Journals |
| issn | 1020-4989 1680-5348 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Pan American Health Organization |
| record_format | Article |
| series | Revista Panamericana de Salud Pública |
| spelling | doaj-art-ea91b5d7bc6d4bae97bac053c7cdd9fa2025-08-20T02:30:28ZengPan American Health OrganizationRevista Panamericana de Salud Pública1020-49891680-53482024-12-01481281510.26633/RPSP.2024.128rpspHigh frequency of HPV high-risk preventable genotypes in Ecuadorian women with invasive cervical cancerGustavo David García Muentes0Fadi Abdul Latif1Claudia Catalina Beltrán Rodríguez2Sebastián Medina González3Juan Carlos Ruiz Cabezas4Sociedad de Lucha Contra el Cáncer (SOLCA), Guayaquil, EcuadorMSD Colombia, Bogotá, ColombiaMSD Colombia, Bogotá, ColombiaMSD Colombia, Bogotá, ColombiaUniversidad de Especialidades Espíritu Santo (UEES), Samborondón, EcuadorObjective. To determine the distribution of human papillomavirus (HPV) genotypes in invasive cervical cancer samples from Ecuadorian women who attended the Cancer Institute (Sociedad de Lucha Contra el Cáncer – SOLCA). Methods. Archived formalin-fixed paraffin-embedded (FFPE) cervical cancer tissue samples collected during 2017–2021 were deparaffinized, and nucleic acid extraction and purification was performed using silica columns. The obtained nucleic acids were analyzed using INNO-LiPA® HPV Genotyping Extra II per the manufacturer’s specifications. Data were retrieved from records, and HPV genotypes were determined from the FFPE samples. Results. The study included samples from 190 women diagnosed with invasive cervical cancer, with a median age of 52.78 years. Squamous cell carcinoma accounted for 78.94% of the cases, while 21.05% had adenocarcinoma. Among the 190 samples, 80.53% tested positive for HPV DNA, while 19.47% were negative. The most common genotypes detected were HPV 16 (64.05%), 18 (16.99%), and 58 (6.54%). HPV infection frequency was higher in samples from patients with elementary level education (p < 0.05). Conclusions. This study provides valuable insights into the distribution of HPV genotypes in invasive cervical cancer samples from Ecuadorian women. The results indicate an elevated presence of HPV 16, HPV 18, and HPV 58, which are vaccine-preventable genotypes.https://iris.paho.org/handle/10665.2/62660papillomavirus infectionsuterine cervical neoplasmsecuador |
| spellingShingle | Gustavo David García Muentes Fadi Abdul Latif Claudia Catalina Beltrán Rodríguez Sebastián Medina González Juan Carlos Ruiz Cabezas High frequency of HPV high-risk preventable genotypes in Ecuadorian women with invasive cervical cancer Revista Panamericana de Salud Pública papillomavirus infections uterine cervical neoplasms ecuador |
| title | High frequency of HPV high-risk preventable genotypes in Ecuadorian women with invasive cervical cancer |
| title_full | High frequency of HPV high-risk preventable genotypes in Ecuadorian women with invasive cervical cancer |
| title_fullStr | High frequency of HPV high-risk preventable genotypes in Ecuadorian women with invasive cervical cancer |
| title_full_unstemmed | High frequency of HPV high-risk preventable genotypes in Ecuadorian women with invasive cervical cancer |
| title_short | High frequency of HPV high-risk preventable genotypes in Ecuadorian women with invasive cervical cancer |
| title_sort | high frequency of hpv high risk preventable genotypes in ecuadorian women with invasive cervical cancer |
| topic | papillomavirus infections uterine cervical neoplasms ecuador |
| url | https://iris.paho.org/handle/10665.2/62660 |
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