Protocols to co-culture human primary lung cells in the simple-flow device
Summary: Human lung models replicate various aspects to address diverse research questions. The complexity of human lung models, such as co-cultures and lung-on-chip devices, is increasing, but details on culture methodologies are often lacking. Here, we describe steps for the isolation, maintenance...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-09-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725002989 |
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| author | Cinta Iriondo Sem Koornneef Kari-Pekka Skarp Marjon Buscop-van Kempen Anne Boerema-de Munck Robbert J. Rottier |
| author_facet | Cinta Iriondo Sem Koornneef Kari-Pekka Skarp Marjon Buscop-van Kempen Anne Boerema-de Munck Robbert J. Rottier |
| author_sort | Cinta Iriondo |
| collection | DOAJ |
| description | Summary: Human lung models replicate various aspects to address diverse research questions. The complexity of human lung models, such as co-cultures and lung-on-chip devices, is increasing, but details on culture methodologies are often lacking. Here, we describe steps for the isolation, maintenance, and co-culturing of primary epithelial, endothelial, and mesenchymal cells derived from human lung resection material. We then detail procedures for 3D printing the simple-flow device, setting it up with co-cultures of human primary epithelial and endothelial cells under fluidic conditions. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-ea896e9d51494acd94f8d3426dd00a57 |
| institution | OA Journals |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-ea896e9d51494acd94f8d3426dd00a572025-08-20T02:10:03ZengElsevierSTAR Protocols2666-16672025-09-016310389210.1016/j.xpro.2025.103892Protocols to co-culture human primary lung cells in the simple-flow deviceCinta Iriondo0Sem Koornneef1Kari-Pekka Skarp2Marjon Buscop-van Kempen3Anne Boerema-de Munck4Robbert J. Rottier5Department of Pediatric Surgery, Sophia Children’s Hospital, Erasmus Medical Center, Rotterdam, the Netherlands; Department of Cell Biology, Erasmus Medical Center, Rotterdam, the Netherlands; Corresponding authorDepartment of Pediatric Surgery, Sophia Children’s Hospital, Erasmus Medical Center, Rotterdam, the Netherlands; Department of Cell Biology, Erasmus Medical Center, Rotterdam, the NetherlandsDepartment of Pediatric Surgery, Sophia Children’s Hospital, Erasmus Medical Center, Rotterdam, the Netherlands; Department of Cell Biology, Erasmus Medical Center, Rotterdam, the NetherlandsDepartment of Pediatric Surgery, Sophia Children’s Hospital, Erasmus Medical Center, Rotterdam, the Netherlands; Department of Cell Biology, Erasmus Medical Center, Rotterdam, the NetherlandsDepartment of Pediatric Surgery, Sophia Children’s Hospital, Erasmus Medical Center, Rotterdam, the Netherlands; Department of Cell Biology, Erasmus Medical Center, Rotterdam, the NetherlandsDepartment of Pediatric Surgery, Sophia Children’s Hospital, Erasmus Medical Center, Rotterdam, the Netherlands; Department of Cell Biology, Erasmus Medical Center, Rotterdam, the Netherlands; Corresponding authorSummary: Human lung models replicate various aspects to address diverse research questions. The complexity of human lung models, such as co-cultures and lung-on-chip devices, is increasing, but details on culture methodologies are often lacking. Here, we describe steps for the isolation, maintenance, and co-culturing of primary epithelial, endothelial, and mesenchymal cells derived from human lung resection material. We then detail procedures for 3D printing the simple-flow device, setting it up with co-cultures of human primary epithelial and endothelial cells under fluidic conditions. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725002989Cell cultureCell isolationBiotechnology and bioengineering |
| spellingShingle | Cinta Iriondo Sem Koornneef Kari-Pekka Skarp Marjon Buscop-van Kempen Anne Boerema-de Munck Robbert J. Rottier Protocols to co-culture human primary lung cells in the simple-flow device STAR Protocols Cell culture Cell isolation Biotechnology and bioengineering |
| title | Protocols to co-culture human primary lung cells in the simple-flow device |
| title_full | Protocols to co-culture human primary lung cells in the simple-flow device |
| title_fullStr | Protocols to co-culture human primary lung cells in the simple-flow device |
| title_full_unstemmed | Protocols to co-culture human primary lung cells in the simple-flow device |
| title_short | Protocols to co-culture human primary lung cells in the simple-flow device |
| title_sort | protocols to co culture human primary lung cells in the simple flow device |
| topic | Cell culture Cell isolation Biotechnology and bioengineering |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725002989 |
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