Protocols to co-culture human primary lung cells in the simple-flow device
Summary: Human lung models replicate various aspects to address diverse research questions. The complexity of human lung models, such as co-cultures and lung-on-chip devices, is increasing, but details on culture methodologies are often lacking. Here, we describe steps for the isolation, maintenance...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725002989 |
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| Summary: | Summary: Human lung models replicate various aspects to address diverse research questions. The complexity of human lung models, such as co-cultures and lung-on-chip devices, is increasing, but details on culture methodologies are often lacking. Here, we describe steps for the isolation, maintenance, and co-culturing of primary epithelial, endothelial, and mesenchymal cells derived from human lung resection material. We then detail procedures for 3D printing the simple-flow device, setting it up with co-cultures of human primary epithelial and endothelial cells under fluidic conditions. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
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| ISSN: | 2666-1667 |