Exploring the growing phase forest musk deer (Moschus berezovskii) dietary energy requirements based on growth performance and gut microbiota analysis

ABSTRACT The forest musk deer (FMD) farming industry is currently experiencing rapid growth, yet the dietary energy requirements for FMD remain unclear. Therefore, we explored the optimal dietary digestible energy (DE) for growing phase FMD by providing three diets with different DE levels and analy...

Full description

Saved in:
Bibliographic Details
Main Authors: Ruiguang Gong, Xiaocao Di, Xinxin Ping, Haodong Han, Bing Song, Shuhui Wang, Xianggui Dong, Zhan Jun Ren
Format: Article
Language:English
Published: American Society for Microbiology 2025-03-01
Series:Microbiology Spectrum
Subjects:
Online Access:https://journals.asm.org/doi/10.1128/spectrum.02352-24
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849705718388621312
author Ruiguang Gong
Xiaocao Di
Xinxin Ping
Haodong Han
Bing Song
Shuhui Wang
Xianggui Dong
Zhan Jun Ren
author_facet Ruiguang Gong
Xiaocao Di
Xinxin Ping
Haodong Han
Bing Song
Shuhui Wang
Xianggui Dong
Zhan Jun Ren
author_sort Ruiguang Gong
collection DOAJ
description ABSTRACT The forest musk deer (FMD) farming industry is currently experiencing rapid growth, yet the dietary energy requirements for FMD remain unclear. Therefore, we explored the optimal dietary digestible energy (DE) for growing phase FMD by providing three diets with different DE levels and analyzing changes in gut microbiota. A total of 20 six-month-old FMD were used in a 62-day trial. Animals were fed either traditional feed (CON, 10.38 MJ/kg DE) or pelleted diets with low (L, 8.87 MJ/kg), medium (M, 10.38 MJ/kg), or high (H, 11.86 MJ/kg) DE levels. The results showed that feeding pelleted diets significantly reduced the incidence of diarrhea (P < 0.05). The average daily gain in the H group was significantly higher than in the M group, and the M group was higher than both the L and CON groups (P < 0.05). Additionally, the H group showed the highest nutrient digestibility and the greatest increase in body measurements (P < 0.05). Analysis of fecal microbiota revealed that α-diversity and the relative abundance of Firmicutes increased with higher dietary energy levels, while the relative abundance of Bacteroidetes decreased. Beneficial bacteria such as Akkermansia and Lachnospira were significantly enriched in the H group. In conclusion, our findings suggest that pelleted diets are suitable for growing phase FMD, with an optimal dietary DE level of 11.86 MJ/kg.IMPORTANCEThis study underscores the significance of identifying the optimal dietary digestible energy (DE) for growing forest musk deer (FMD). Pelleted diets with a DE level of 11.86 MJ/kg enhanced growth performance, nutrient digestibility, and gut health, while reducing diarrhea and enriching beneficial gut bacteria, offering valuable insights for improving FMD farming practices.
format Article
id doaj-art-ea87f6bb454c40dcb0a9acd6aa1ce3dc
institution DOAJ
issn 2165-0497
language English
publishDate 2025-03-01
publisher American Society for Microbiology
record_format Article
series Microbiology Spectrum
spelling doaj-art-ea87f6bb454c40dcb0a9acd6aa1ce3dc2025-08-20T03:16:24ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972025-03-0113310.1128/spectrum.02352-24Exploring the growing phase forest musk deer (Moschus berezovskii) dietary energy requirements based on growth performance and gut microbiota analysisRuiguang Gong0Xiaocao Di1Xinxin Ping2Haodong Han3Bing Song4Shuhui Wang5Xianggui Dong6Zhan Jun Ren7College of Animal Science and Technology, Northwest A&amp;F University, Yangling, ChinaCollege of Animal Science and Technology, Northwest A&amp;F University, Yangling, ChinaCollege of Animal Science and Technology, Northwest A&amp;F University, Yangling, ChinaCollege of Animal Science and Technology, Northwest A&amp;F University, Yangling, ChinaCollege of Animal Science and Technology, Northwest A&amp;F University, Yangling, ChinaCollege of Animal Science and Technology, Northwest A&amp;F University, Yangling, ChinaCollege of Animal Science and Technology, Northwest A&amp;F University, Yangling, ChinaCollege of Animal Science and Technology, Northwest A&amp;F University, Yangling, ChinaABSTRACT The forest musk deer (FMD) farming industry is currently experiencing rapid growth, yet the dietary energy requirements for FMD remain unclear. Therefore, we explored the optimal dietary digestible energy (DE) for growing phase FMD by providing three diets with different DE levels and analyzing changes in gut microbiota. A total of 20 six-month-old FMD were used in a 62-day trial. Animals were fed either traditional feed (CON, 10.38 MJ/kg DE) or pelleted diets with low (L, 8.87 MJ/kg), medium (M, 10.38 MJ/kg), or high (H, 11.86 MJ/kg) DE levels. The results showed that feeding pelleted diets significantly reduced the incidence of diarrhea (P < 0.05). The average daily gain in the H group was significantly higher than in the M group, and the M group was higher than both the L and CON groups (P < 0.05). Additionally, the H group showed the highest nutrient digestibility and the greatest increase in body measurements (P < 0.05). Analysis of fecal microbiota revealed that α-diversity and the relative abundance of Firmicutes increased with higher dietary energy levels, while the relative abundance of Bacteroidetes decreased. Beneficial bacteria such as Akkermansia and Lachnospira were significantly enriched in the H group. In conclusion, our findings suggest that pelleted diets are suitable for growing phase FMD, with an optimal dietary DE level of 11.86 MJ/kg.IMPORTANCEThis study underscores the significance of identifying the optimal dietary digestible energy (DE) for growing forest musk deer (FMD). Pelleted diets with a DE level of 11.86 MJ/kg enhanced growth performance, nutrient digestibility, and gut health, while reducing diarrhea and enriching beneficial gut bacteria, offering valuable insights for improving FMD farming practices.https://journals.asm.org/doi/10.1128/spectrum.02352-24Moschus berezovskiidigestible energygrowth performancefecal microbiota16S rRNA
spellingShingle Ruiguang Gong
Xiaocao Di
Xinxin Ping
Haodong Han
Bing Song
Shuhui Wang
Xianggui Dong
Zhan Jun Ren
Exploring the growing phase forest musk deer (Moschus berezovskii) dietary energy requirements based on growth performance and gut microbiota analysis
Microbiology Spectrum
Moschus berezovskii
digestible energy
growth performance
fecal microbiota
16S rRNA
title Exploring the growing phase forest musk deer (Moschus berezovskii) dietary energy requirements based on growth performance and gut microbiota analysis
title_full Exploring the growing phase forest musk deer (Moschus berezovskii) dietary energy requirements based on growth performance and gut microbiota analysis
title_fullStr Exploring the growing phase forest musk deer (Moschus berezovskii) dietary energy requirements based on growth performance and gut microbiota analysis
title_full_unstemmed Exploring the growing phase forest musk deer (Moschus berezovskii) dietary energy requirements based on growth performance and gut microbiota analysis
title_short Exploring the growing phase forest musk deer (Moschus berezovskii) dietary energy requirements based on growth performance and gut microbiota analysis
title_sort exploring the growing phase forest musk deer moschus berezovskii dietary energy requirements based on growth performance and gut microbiota analysis
topic Moschus berezovskii
digestible energy
growth performance
fecal microbiota
16S rRNA
url https://journals.asm.org/doi/10.1128/spectrum.02352-24
work_keys_str_mv AT ruiguanggong exploringthegrowingphaseforestmuskdeermoschusberezovskiidietaryenergyrequirementsbasedongrowthperformanceandgutmicrobiotaanalysis
AT xiaocaodi exploringthegrowingphaseforestmuskdeermoschusberezovskiidietaryenergyrequirementsbasedongrowthperformanceandgutmicrobiotaanalysis
AT xinxinping exploringthegrowingphaseforestmuskdeermoschusberezovskiidietaryenergyrequirementsbasedongrowthperformanceandgutmicrobiotaanalysis
AT haodonghan exploringthegrowingphaseforestmuskdeermoschusberezovskiidietaryenergyrequirementsbasedongrowthperformanceandgutmicrobiotaanalysis
AT bingsong exploringthegrowingphaseforestmuskdeermoschusberezovskiidietaryenergyrequirementsbasedongrowthperformanceandgutmicrobiotaanalysis
AT shuhuiwang exploringthegrowingphaseforestmuskdeermoschusberezovskiidietaryenergyrequirementsbasedongrowthperformanceandgutmicrobiotaanalysis
AT xiangguidong exploringthegrowingphaseforestmuskdeermoschusberezovskiidietaryenergyrequirementsbasedongrowthperformanceandgutmicrobiotaanalysis
AT zhanjunren exploringthegrowingphaseforestmuskdeermoschusberezovskiidietaryenergyrequirementsbasedongrowthperformanceandgutmicrobiotaanalysis