Molecular Regulation of Carotenoid Accumulation Enhanced by Oxidative Stress in the Food Industrial Strain <i>Blakeslea trispora</i>
<i>Blakeslea trispora</i> is a key industrial strain for carotenoid production due to its rapid growth, ease of cultivation, and high yield. This study examined the effects of oxidative stress induced by rose bengal (RB) and hydrogen peroxide (H<sub>2</sub>O<sub>2</s...
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MDPI AG
2025-04-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/14/9/1452 |
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| author | Jiawei Deng Yuyang Chen Siting Lin Yilu Shao Yuan Zou Qianwang Zheng Liqiong Guo Junfang Lin Moutong Chen Zhiwei Ye |
| author_facet | Jiawei Deng Yuyang Chen Siting Lin Yilu Shao Yuan Zou Qianwang Zheng Liqiong Guo Junfang Lin Moutong Chen Zhiwei Ye |
| author_sort | Jiawei Deng |
| collection | DOAJ |
| description | <i>Blakeslea trispora</i> is a key industrial strain for carotenoid production due to its rapid growth, ease of cultivation, and high yield. This study examined the effects of oxidative stress induced by rose bengal (RB) and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) on carotenoid accumulation, achieving maximum yields of 459.38 ± 77.15 μg/g dry cell weight (DCW) at 0.4 g/L RB and 294.38 ± 14.16 μg/g DCW at 0.6% H<sub>2</sub>O<sub>2</sub>. These results demonstrate that oxidative stress promotes carotenoid accumulation in <i>B. trispora</i>. To investigate the underlying molecular mechanisms, transcriptional levels of key genes were analyzed under optimal stress conditions. In the carotenogenic pathway, only <i>HMGR</i> showed upregulation, while <i>ACC</i>, linked to fatty acid biosynthesis, remained unchanged. Within the mitogen-activated protein kinase (MAPK) pathway, <i>FUS3</i> transcription increased under both stress conditions, <i>MPK1</i> transcription rose only under H<sub>2</sub>O<sub>2</sub> stress, and <i>HOG1</i> exhibited no significant changes. Among heat shock proteins (HSPs), only <i>HSP70</i> showed elevated transcription under H<sub>2</sub>O<sub>2</sub> stress, while other <i>HSP</i> genes remained unchanged. These findings suggest that oxidative stress induced by RB and H<sub>2</sub>O<sub>2</sub> enhances carotenoid accumulation in <i>B. trispora</i> through distinct regulatory pathways. This study provides valuable insights into stress-adaptive mechanisms and offers strategies to optimize carotenoid production in fungi. |
| format | Article |
| id | doaj-art-ea869bf1554542129a558d922eac51c3 |
| institution | DOAJ |
| issn | 2304-8158 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj-art-ea869bf1554542129a558d922eac51c32025-08-20T02:58:44ZengMDPI AGFoods2304-81582025-04-01149145210.3390/foods14091452Molecular Regulation of Carotenoid Accumulation Enhanced by Oxidative Stress in the Food Industrial Strain <i>Blakeslea trispora</i>Jiawei Deng0Yuyang Chen1Siting Lin2Yilu Shao3Yuan Zou4Qianwang Zheng5Liqiong Guo6Junfang Lin7Moutong Chen8Zhiwei Ye9College of Food Science, South China Agricultural University, Guangzhou 510642, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510642, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510642, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510642, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510642, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510642, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510642, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510642, China<i>Blakeslea trispora</i> is a key industrial strain for carotenoid production due to its rapid growth, ease of cultivation, and high yield. This study examined the effects of oxidative stress induced by rose bengal (RB) and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) on carotenoid accumulation, achieving maximum yields of 459.38 ± 77.15 μg/g dry cell weight (DCW) at 0.4 g/L RB and 294.38 ± 14.16 μg/g DCW at 0.6% H<sub>2</sub>O<sub>2</sub>. These results demonstrate that oxidative stress promotes carotenoid accumulation in <i>B. trispora</i>. To investigate the underlying molecular mechanisms, transcriptional levels of key genes were analyzed under optimal stress conditions. In the carotenogenic pathway, only <i>HMGR</i> showed upregulation, while <i>ACC</i>, linked to fatty acid biosynthesis, remained unchanged. Within the mitogen-activated protein kinase (MAPK) pathway, <i>FUS3</i> transcription increased under both stress conditions, <i>MPK1</i> transcription rose only under H<sub>2</sub>O<sub>2</sub> stress, and <i>HOG1</i> exhibited no significant changes. Among heat shock proteins (HSPs), only <i>HSP70</i> showed elevated transcription under H<sub>2</sub>O<sub>2</sub> stress, while other <i>HSP</i> genes remained unchanged. These findings suggest that oxidative stress induced by RB and H<sub>2</sub>O<sub>2</sub> enhances carotenoid accumulation in <i>B. trispora</i> through distinct regulatory pathways. This study provides valuable insights into stress-adaptive mechanisms and offers strategies to optimize carotenoid production in fungi.https://www.mdpi.com/2304-8158/14/9/1452<i>Blakeslea trispora</i>oxidative stresscarotenoidsbiosynthesisheat shock protein |
| spellingShingle | Jiawei Deng Yuyang Chen Siting Lin Yilu Shao Yuan Zou Qianwang Zheng Liqiong Guo Junfang Lin Moutong Chen Zhiwei Ye Molecular Regulation of Carotenoid Accumulation Enhanced by Oxidative Stress in the Food Industrial Strain <i>Blakeslea trispora</i> Foods <i>Blakeslea trispora</i> oxidative stress carotenoids biosynthesis heat shock protein |
| title | Molecular Regulation of Carotenoid Accumulation Enhanced by Oxidative Stress in the Food Industrial Strain <i>Blakeslea trispora</i> |
| title_full | Molecular Regulation of Carotenoid Accumulation Enhanced by Oxidative Stress in the Food Industrial Strain <i>Blakeslea trispora</i> |
| title_fullStr | Molecular Regulation of Carotenoid Accumulation Enhanced by Oxidative Stress in the Food Industrial Strain <i>Blakeslea trispora</i> |
| title_full_unstemmed | Molecular Regulation of Carotenoid Accumulation Enhanced by Oxidative Stress in the Food Industrial Strain <i>Blakeslea trispora</i> |
| title_short | Molecular Regulation of Carotenoid Accumulation Enhanced by Oxidative Stress in the Food Industrial Strain <i>Blakeslea trispora</i> |
| title_sort | molecular regulation of carotenoid accumulation enhanced by oxidative stress in the food industrial strain i blakeslea trispora i |
| topic | <i>Blakeslea trispora</i> oxidative stress carotenoids biosynthesis heat shock protein |
| url | https://www.mdpi.com/2304-8158/14/9/1452 |
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