Quantitative Estimation of Sorafenib Tosylate Its Pure Form and in Its Tablet Formulation by RP-HPLC Method

A simple, accurate, specific reverse-phase, high-performance liquid chromatography method has been developed for the determination of sorafenib tosylate in its pure form and its tablets. In this method, sorafenib tosylate was eluted by isocratic mode using a Phenomenex Luna C18 column by a mobile ph...

Full description

Saved in:
Bibliographic Details
Main Authors: R. Kalaichelvi, E. Jayachandran
Format: Article
Language:English
Published: Wiley 2013-01-01
Series:Journal of Chemistry
Online Access:http://dx.doi.org/10.1155/2013/539264
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849692497516691456
author R. Kalaichelvi
E. Jayachandran
author_facet R. Kalaichelvi
E. Jayachandran
author_sort R. Kalaichelvi
collection DOAJ
description A simple, accurate, specific reverse-phase, high-performance liquid chromatography method has been developed for the determination of sorafenib tosylate in its pure form and its tablets. In this method, sorafenib tosylate was eluted by isocratic mode using a Phenomenex Luna C18 column by a mobile phase composition of acetonitrile and water in the ratio of 82.5 : 17.5, v/v. The flow rate was 1.5 mL/min. The eluted drug was monitored at 265 nm and the method was found to be linear from 5 to 80 μg/mL. The method was validated by linearity, precision, accuracy, LOD, and LOQ. The accuracy report denotes that there is not any interference of additives used in the formulation.
format Article
id doaj-art-ea79062f5d8c4499b9c8874f9260dbb4
institution DOAJ
issn 2090-9063
2090-9071
language English
publishDate 2013-01-01
publisher Wiley
record_format Article
series Journal of Chemistry
spelling doaj-art-ea79062f5d8c4499b9c8874f9260dbb42025-08-20T03:20:40ZengWileyJournal of Chemistry2090-90632090-90712013-01-01201310.1155/2013/539264539264Quantitative Estimation of Sorafenib Tosylate Its Pure Form and in Its Tablet Formulation by RP-HPLC MethodR. Kalaichelvi0E. Jayachandran1Department of Pharmaceutical Analysis, K. C. Reddy Institute of Pharmaceutical Sciences, Jangamguntla Palem, Medikonduru Mandal, Guntur 522 348, IndiaDepartment of Pharmaceutical Chemistry, S.C.S. College of Pharmacy, Devanagari District, Karnataka, Harapanahalli 583131, IndiaA simple, accurate, specific reverse-phase, high-performance liquid chromatography method has been developed for the determination of sorafenib tosylate in its pure form and its tablets. In this method, sorafenib tosylate was eluted by isocratic mode using a Phenomenex Luna C18 column by a mobile phase composition of acetonitrile and water in the ratio of 82.5 : 17.5, v/v. The flow rate was 1.5 mL/min. The eluted drug was monitored at 265 nm and the method was found to be linear from 5 to 80 μg/mL. The method was validated by linearity, precision, accuracy, LOD, and LOQ. The accuracy report denotes that there is not any interference of additives used in the formulation.http://dx.doi.org/10.1155/2013/539264
spellingShingle R. Kalaichelvi
E. Jayachandran
Quantitative Estimation of Sorafenib Tosylate Its Pure Form and in Its Tablet Formulation by RP-HPLC Method
Journal of Chemistry
title Quantitative Estimation of Sorafenib Tosylate Its Pure Form and in Its Tablet Formulation by RP-HPLC Method
title_full Quantitative Estimation of Sorafenib Tosylate Its Pure Form and in Its Tablet Formulation by RP-HPLC Method
title_fullStr Quantitative Estimation of Sorafenib Tosylate Its Pure Form and in Its Tablet Formulation by RP-HPLC Method
title_full_unstemmed Quantitative Estimation of Sorafenib Tosylate Its Pure Form and in Its Tablet Formulation by RP-HPLC Method
title_short Quantitative Estimation of Sorafenib Tosylate Its Pure Form and in Its Tablet Formulation by RP-HPLC Method
title_sort quantitative estimation of sorafenib tosylate its pure form and in its tablet formulation by rp hplc method
url http://dx.doi.org/10.1155/2013/539264
work_keys_str_mv AT rkalaichelvi quantitativeestimationofsorafenibtosylateitspureformandinitstabletformulationbyrphplcmethod
AT ejayachandran quantitativeestimationofsorafenibtosylateitspureformandinitstabletformulationbyrphplcmethod