Cryomicroneedle Arrays for Biotherapeutics Delivery

Biotherapy offers a promising approach for treating a variety of diseases. However, the lack of advanced delivery systems remains a significant barrier to improve the efficacy, safety, and cost‐effectiveness of biotherapeutics. The microneedle, as a minimally invasive drug delivery tool, has demonst...

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Main Authors: Chunli Yang, Li Zhang, Angxi Zhou, Siyi Wang, Ya Ren, Maya Xiang, Run Tian, Yang Yu, Rong Li, Maling Gou
Format: Article
Language:English
Published: Wiley-VCH 2025-08-01
Series:Small Science
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Online Access:https://doi.org/10.1002/smsc.202500009
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author Chunli Yang
Li Zhang
Angxi Zhou
Siyi Wang
Ya Ren
Maya Xiang
Run Tian
Yang Yu
Rong Li
Maling Gou
author_facet Chunli Yang
Li Zhang
Angxi Zhou
Siyi Wang
Ya Ren
Maya Xiang
Run Tian
Yang Yu
Rong Li
Maling Gou
author_sort Chunli Yang
collection DOAJ
description Biotherapy offers a promising approach for treating a variety of diseases. However, the lack of advanced delivery systems remains a significant barrier to improve the efficacy, safety, and cost‐effectiveness of biotherapeutics. The microneedle, as a minimally invasive drug delivery tool, has demonstrated considerable potential in biotherapeutic applications. Despite this promise, challenges remain in fabricating microneedles that effectively preserve the bioactivity of biotherapeutics. Emerging as a novel solution, cryomicroneedles (cryoMNs) employ cryogenically molded ice matrices that exploit phase‐transition thermodynamics. The metabolic stasis induced by cryoimmobilization preserves biomolecular conformation and cellular viability. Moreover, the ice‐reinforced architectures achieve an optimal balance between mechanical penetration capacity and post‐insertion dissolution kinetics, overcoming the rigidity‐flexibility trade‐off in traditional dissolving microneedles. Current research prioritizes three breakthrough directions: material innovation for cryocompatible polymer‐ice interfaces, cold‐chain optimization strategies to enhance payload viability, and innovations in medical application scenarios. Notably, preclinical successes in regenerative tissue engineering and thermostable vaccine platforms highlight cryoMNs’ potential to bridge precision medicine and global health equity. This review provides an overview of recent advancements in cryoMNs and discusses the potential challenges and future directions for the development of cryoMNs‐mediated biotherapeutics delivery.
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spelling doaj-art-ea740b1ded9641a38591d8d07a9d8c8d2025-08-20T03:36:09ZengWiley-VCHSmall Science2688-40462025-08-0158n/an/a10.1002/smsc.202500009Cryomicroneedle Arrays for Biotherapeutics DeliveryChunli Yang0Li Zhang1Angxi Zhou2Siyi Wang3Ya Ren4Maya Xiang5Run Tian6Yang Yu7Rong Li8Maling Gou9Department of Biotherapy Cancer Center and State Key Laboratory of Biotherapy West China Hospital Sichuan University Chengdu Sichuan Province 610041 ChinaDepartment of Biotherapy Cancer Center and State Key Laboratory of Biotherapy West China Hospital Sichuan University Chengdu Sichuan Province 610041 ChinaDepartment of Biotherapy Cancer Center and State Key Laboratory of Biotherapy West China Hospital Sichuan University Chengdu Sichuan Province 610041 ChinaDepartment of Biotherapy Cancer Center and State Key Laboratory of Biotherapy West China Hospital Sichuan University Chengdu Sichuan Province 610041 ChinaDepartment of Biotherapy Cancer Center and State Key Laboratory of Biotherapy West China Hospital Sichuan University Chengdu Sichuan Province 610041 ChinaDepartment of Chemistry University of Washington‐Seattle Campus Seattle WA 98195 USADepartment of Dermatology West China Hospital Sichuan University Chengdu Sichuan Province 610041 ChinaDepartment of Thoracic Oncology Cancer Center and State Key Laboratory of Biotherapy West China Hospital Sichuan University Chengdu Sichuan Province 610041 ChinaAntibiotics Research and Re‐evaluation Key Laboratory of Sichuan Province Sichuan Industrial Institute of Antibiotics School of Pharmacy Chengdu University Chengdu Sichuan Province 610041 ChinaDepartment of Biotherapy Cancer Center and State Key Laboratory of Biotherapy West China Hospital Sichuan University Chengdu Sichuan Province 610041 ChinaBiotherapy offers a promising approach for treating a variety of diseases. However, the lack of advanced delivery systems remains a significant barrier to improve the efficacy, safety, and cost‐effectiveness of biotherapeutics. The microneedle, as a minimally invasive drug delivery tool, has demonstrated considerable potential in biotherapeutic applications. Despite this promise, challenges remain in fabricating microneedles that effectively preserve the bioactivity of biotherapeutics. Emerging as a novel solution, cryomicroneedles (cryoMNs) employ cryogenically molded ice matrices that exploit phase‐transition thermodynamics. The metabolic stasis induced by cryoimmobilization preserves biomolecular conformation and cellular viability. Moreover, the ice‐reinforced architectures achieve an optimal balance between mechanical penetration capacity and post‐insertion dissolution kinetics, overcoming the rigidity‐flexibility trade‐off in traditional dissolving microneedles. Current research prioritizes three breakthrough directions: material innovation for cryocompatible polymer‐ice interfaces, cold‐chain optimization strategies to enhance payload viability, and innovations in medical application scenarios. Notably, preclinical successes in regenerative tissue engineering and thermostable vaccine platforms highlight cryoMNs’ potential to bridge precision medicine and global health equity. This review provides an overview of recent advancements in cryoMNs and discusses the potential challenges and future directions for the development of cryoMNs‐mediated biotherapeutics delivery.https://doi.org/10.1002/smsc.202500009biotherapeuticscryomicroneedlesdiseasesdrug deliverytreatment
spellingShingle Chunli Yang
Li Zhang
Angxi Zhou
Siyi Wang
Ya Ren
Maya Xiang
Run Tian
Yang Yu
Rong Li
Maling Gou
Cryomicroneedle Arrays for Biotherapeutics Delivery
Small Science
biotherapeutics
cryomicroneedles
diseases
drug delivery
treatment
title Cryomicroneedle Arrays for Biotherapeutics Delivery
title_full Cryomicroneedle Arrays for Biotherapeutics Delivery
title_fullStr Cryomicroneedle Arrays for Biotherapeutics Delivery
title_full_unstemmed Cryomicroneedle Arrays for Biotherapeutics Delivery
title_short Cryomicroneedle Arrays for Biotherapeutics Delivery
title_sort cryomicroneedle arrays for biotherapeutics delivery
topic biotherapeutics
cryomicroneedles
diseases
drug delivery
treatment
url https://doi.org/10.1002/smsc.202500009
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AT yaren cryomicroneedlearraysforbiotherapeuticsdelivery
AT mayaxiang cryomicroneedlearraysforbiotherapeuticsdelivery
AT runtian cryomicroneedlearraysforbiotherapeuticsdelivery
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