Bacteriophage and Phage-Encoded Depolymerase Exhibit Antibacterial Activity Against K9-Type <i>Acinetobacter baumannii</i> in Mouse Sepsis and Burn Skin Infection Models
<i>Acinetobacter baumannii</i> is a widely distributed nosocomial pathogen that causes various acute and chronic infections, particularly in immunocompromised patients. In this study, the activities of the K9-specific virulent phage AM24 and phage-encoded depolymerase DepAPK09 were asses...
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2025-01-01
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| author | Alexander I. Borzilov Nikolay V. Volozhantsev Olga V. Korobova Lyubov V. Kolupaeva Evgenia S. Pereskokova Tatiana I. Kombarova Mikhail M. Shneider Konstantin A. Miroshnikov Ivan A. Dyatlov Anastasia V. Popova |
| author_facet | Alexander I. Borzilov Nikolay V. Volozhantsev Olga V. Korobova Lyubov V. Kolupaeva Evgenia S. Pereskokova Tatiana I. Kombarova Mikhail M. Shneider Konstantin A. Miroshnikov Ivan A. Dyatlov Anastasia V. Popova |
| author_sort | Alexander I. Borzilov |
| collection | DOAJ |
| description | <i>Acinetobacter baumannii</i> is a widely distributed nosocomial pathogen that causes various acute and chronic infections, particularly in immunocompromised patients. In this study, the activities of the K9-specific virulent phage AM24 and phage-encoded depolymerase DepAPK09 were assessed using in vivo mouse sepsis and burn skin infection models. In the mouse sepsis model, in the case of prevention or early treatment, a single K9-specific phage or recombinant depolymerase injection was able to protect 100% of the mice after parenteral infection with a lethal dose of <i>A. baumannii</i> of the K9-type, with complete eradication of the pathogen. In the case of delayed treatment, mouse survival decreased to 70% when injected with the phage and to 40% when treated with the recombinant enzyme. In the mouse burn skin infection model, the number of <i>A. baumannii</i> cells on the surface of the wound and in the deep layers of the skin decreased by several-fold after treatment with both the K9-specific phage and the recombinant depolymerase. The phage and recombinant depolymerase were highly stable and retained activity under a wide range of temperatures and pH values. The results obtained contribute to expanding our understanding of the in vivo therapeutic potential of specific phages and phage-derived depolymerases interacting with <i>A. baumannii</i> of different capsular types. |
| format | Article |
| id | doaj-art-ea722a3b2ac04a559c3bb91c62c9e4ce |
| institution | Kabale University |
| issn | 1999-4915 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj-art-ea722a3b2ac04a559c3bb91c62c9e4ce2025-01-24T13:52:28ZengMDPI AGViruses1999-49152025-01-011717010.3390/v17010070Bacteriophage and Phage-Encoded Depolymerase Exhibit Antibacterial Activity Against K9-Type <i>Acinetobacter baumannii</i> in Mouse Sepsis and Burn Skin Infection ModelsAlexander I. Borzilov0Nikolay V. Volozhantsev1Olga V. Korobova2Lyubov V. Kolupaeva3Evgenia S. Pereskokova4Tatiana I. Kombarova5Mikhail M. Shneider6Konstantin A. Miroshnikov7Ivan A. Dyatlov8Anastasia V. Popova9State Research Center for Applied Microbiology and Biotechnology, City District Serpukhov, Moscow Region, 142279 Obolensk, RussiaState Research Center for Applied Microbiology and Biotechnology, City District Serpukhov, Moscow Region, 142279 Obolensk, RussiaState Research Center for Applied Microbiology and Biotechnology, City District Serpukhov, Moscow Region, 142279 Obolensk, RussiaState Research Center for Applied Microbiology and Biotechnology, City District Serpukhov, Moscow Region, 142279 Obolensk, RussiaState Research Center for Applied Microbiology and Biotechnology, City District Serpukhov, Moscow Region, 142279 Obolensk, RussiaState Research Center for Applied Microbiology and Biotechnology, City District Serpukhov, Moscow Region, 142279 Obolensk, RussiaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, RussiaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, RussiaState Research Center for Applied Microbiology and Biotechnology, City District Serpukhov, Moscow Region, 142279 Obolensk, RussiaState Research Center for Applied Microbiology and Biotechnology, City District Serpukhov, Moscow Region, 142279 Obolensk, Russia<i>Acinetobacter baumannii</i> is a widely distributed nosocomial pathogen that causes various acute and chronic infections, particularly in immunocompromised patients. In this study, the activities of the K9-specific virulent phage AM24 and phage-encoded depolymerase DepAPK09 were assessed using in vivo mouse sepsis and burn skin infection models. In the mouse sepsis model, in the case of prevention or early treatment, a single K9-specific phage or recombinant depolymerase injection was able to protect 100% of the mice after parenteral infection with a lethal dose of <i>A. baumannii</i> of the K9-type, with complete eradication of the pathogen. In the case of delayed treatment, mouse survival decreased to 70% when injected with the phage and to 40% when treated with the recombinant enzyme. In the mouse burn skin infection model, the number of <i>A. baumannii</i> cells on the surface of the wound and in the deep layers of the skin decreased by several-fold after treatment with both the K9-specific phage and the recombinant depolymerase. The phage and recombinant depolymerase were highly stable and retained activity under a wide range of temperatures and pH values. The results obtained contribute to expanding our understanding of the in vivo therapeutic potential of specific phages and phage-derived depolymerases interacting with <i>A. baumannii</i> of different capsular types.https://www.mdpi.com/1999-4915/17/1/70<i>Acinetobacter baumannii</i>bacteriophagedepolymerasemouse sepsis modelmouse burn skin infection model |
| spellingShingle | Alexander I. Borzilov Nikolay V. Volozhantsev Olga V. Korobova Lyubov V. Kolupaeva Evgenia S. Pereskokova Tatiana I. Kombarova Mikhail M. Shneider Konstantin A. Miroshnikov Ivan A. Dyatlov Anastasia V. Popova Bacteriophage and Phage-Encoded Depolymerase Exhibit Antibacterial Activity Against K9-Type <i>Acinetobacter baumannii</i> in Mouse Sepsis and Burn Skin Infection Models Viruses <i>Acinetobacter baumannii</i> bacteriophage depolymerase mouse sepsis model mouse burn skin infection model |
| title | Bacteriophage and Phage-Encoded Depolymerase Exhibit Antibacterial Activity Against K9-Type <i>Acinetobacter baumannii</i> in Mouse Sepsis and Burn Skin Infection Models |
| title_full | Bacteriophage and Phage-Encoded Depolymerase Exhibit Antibacterial Activity Against K9-Type <i>Acinetobacter baumannii</i> in Mouse Sepsis and Burn Skin Infection Models |
| title_fullStr | Bacteriophage and Phage-Encoded Depolymerase Exhibit Antibacterial Activity Against K9-Type <i>Acinetobacter baumannii</i> in Mouse Sepsis and Burn Skin Infection Models |
| title_full_unstemmed | Bacteriophage and Phage-Encoded Depolymerase Exhibit Antibacterial Activity Against K9-Type <i>Acinetobacter baumannii</i> in Mouse Sepsis and Burn Skin Infection Models |
| title_short | Bacteriophage and Phage-Encoded Depolymerase Exhibit Antibacterial Activity Against K9-Type <i>Acinetobacter baumannii</i> in Mouse Sepsis and Burn Skin Infection Models |
| title_sort | bacteriophage and phage encoded depolymerase exhibit antibacterial activity against k9 type i acinetobacter baumannii i in mouse sepsis and burn skin infection models |
| topic | <i>Acinetobacter baumannii</i> bacteriophage depolymerase mouse sepsis model mouse burn skin infection model |
| url | https://www.mdpi.com/1999-4915/17/1/70 |
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