Antigenic properties estimation for legumain protein recombinant analogue of trematoda Opisthorchis felineus, causing human opisthorchiasis

We estimated potential of protein legumain from opisthorchiasis agent O. felineus for application in immunodiagnosis of this disease. Bacterially expressed recombinant protein (rOF49-leg) showed strong difference in Enzyme-linked immunosorbent assay (ELISA) between infected and negative sera. These...

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Main Authors: I. A. Razumov, M. N. Lvova, Ye. P. Ponomareva, A. V. Katokhin, V. A. Petrenko, A. E. Sazonov, L. M. Ogorodova, V. V. Novitsky, A. Yu. Sivkov, V. A. Mordvinov
Format: Article
Language:English
Published: Siberian State Medical University (Tomsk) 2012-12-01
Series:Бюллетень сибирской медицины
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Online Access:https://bulletin.ssmu.ru/jour/article/view/897
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author I. A. Razumov
M. N. Lvova
Ye. P. Ponomareva
A. V. Katokhin
V. A. Petrenko
A. E. Sazonov
L. M. Ogorodova
V. V. Novitsky
A. Yu. Sivkov
V. A. Mordvinov
author_facet I. A. Razumov
M. N. Lvova
Ye. P. Ponomareva
A. V. Katokhin
V. A. Petrenko
A. E. Sazonov
L. M. Ogorodova
V. V. Novitsky
A. Yu. Sivkov
V. A. Mordvinov
author_sort I. A. Razumov
collection DOAJ
description We estimated potential of protein legumain from opisthorchiasis agent O. felineus for application in immunodiagnosis of this disease. Bacterially expressed recombinant protein (rOF49-leg) showed strong difference in Enzyme-linked immunosorbent assay (ELISA) between infected and negative sera. These results suggest the potential of this antigen for development of serodiagnostic test for human opisthorchiasis.
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id doaj-art-ea6f6de95ea04364bcc1a388fb26fc79
institution Kabale University
issn 1682-0363
1819-3684
language English
publishDate 2012-12-01
publisher Siberian State Medical University (Tomsk)
record_format Article
series Бюллетень сибирской медицины
spelling doaj-art-ea6f6de95ea04364bcc1a388fb26fc792025-08-20T04:00:19ZengSiberian State Medical University (Tomsk)Бюллетень сибирской медицины1682-03631819-36842012-12-0111616617110.20538/1682-0363-2012-6-166-171686Antigenic properties estimation for legumain protein recombinant analogue of trematoda Opisthorchis felineus, causing human opisthorchiasisI. A. Razumov0M. N. Lvova1Ye. P. Ponomareva2A. V. Katokhin3V. A. Petrenko4A. E. Sazonov5L. M. Ogorodova6V. V. Novitsky7A. Yu. Sivkov8V. A. Mordvinov9Институт цитологии и генетики СО РАН, г. НовосибирскИнститут цитологии и генетики СО РАН, г. НовосибирскИнститут цитологии и генетики СО РАН, г. НовосибирскИнститут цитологии и генетики СО РАН, г. НовосибирскИнститут цитологии и генетики СО РАН, г. НовосибирскИнститут цитологии и генетики СО РАН, г. НовосибирскСибирский государственный медицинский университет, г. ТомскСибирский государственный медицинский университет, г. ТомскИнститут цитологии и генетики СО РАН, г. НовосибирскИнститут цитологии и генетики СО РАН, г. НовосибирскWe estimated potential of protein legumain from opisthorchiasis agent O. felineus for application in immunodiagnosis of this disease. Bacterially expressed recombinant protein (rOF49-leg) showed strong difference in Enzyme-linked immunosorbent assay (ELISA) between infected and negative sera. These results suggest the potential of this antigen for development of serodiagnostic test for human opisthorchiasis.https://bulletin.ssmu.ru/jour/article/view/897opisthorchis felineusopisthorchiasisrecombinant proteinslegumainimmunodiagnosis
spellingShingle I. A. Razumov
M. N. Lvova
Ye. P. Ponomareva
A. V. Katokhin
V. A. Petrenko
A. E. Sazonov
L. M. Ogorodova
V. V. Novitsky
A. Yu. Sivkov
V. A. Mordvinov
Antigenic properties estimation for legumain protein recombinant analogue of trematoda Opisthorchis felineus, causing human opisthorchiasis
Бюллетень сибирской медицины
opisthorchis felineus
opisthorchiasis
recombinant proteins
legumain
immunodiagnosis
title Antigenic properties estimation for legumain protein recombinant analogue of trematoda Opisthorchis felineus, causing human opisthorchiasis
title_full Antigenic properties estimation for legumain protein recombinant analogue of trematoda Opisthorchis felineus, causing human opisthorchiasis
title_fullStr Antigenic properties estimation for legumain protein recombinant analogue of trematoda Opisthorchis felineus, causing human opisthorchiasis
title_full_unstemmed Antigenic properties estimation for legumain protein recombinant analogue of trematoda Opisthorchis felineus, causing human opisthorchiasis
title_short Antigenic properties estimation for legumain protein recombinant analogue of trematoda Opisthorchis felineus, causing human opisthorchiasis
title_sort antigenic properties estimation for legumain protein recombinant analogue of trematoda opisthorchis felineus causing human opisthorchiasis
topic opisthorchis felineus
opisthorchiasis
recombinant proteins
legumain
immunodiagnosis
url https://bulletin.ssmu.ru/jour/article/view/897
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