CD19+CD24hiCD38hi B Cell Dysfunction in Primary Biliary Cholangitis
CD19+CD24hiCD38hi B cells are immature transitional B cells that, in normal individuals, exert suppressive effects by IL-10 production but are quantitatively altered and/or functionally impaired in individuals with various autoimmune diseases. Primary biliary cholangitis (PBC), an autoimmune disease...
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| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2020/3019378 |
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| author | Qubo Chen Lanmin Lai Xiaoling Chi Xinyi Lu Huaxian Wu Jing Sun Weilin Wu Li Cai Xuan Zeng Chuyang Wang WeiCheng Chen Anping Peng |
| author_facet | Qubo Chen Lanmin Lai Xiaoling Chi Xinyi Lu Huaxian Wu Jing Sun Weilin Wu Li Cai Xuan Zeng Chuyang Wang WeiCheng Chen Anping Peng |
| author_sort | Qubo Chen |
| collection | DOAJ |
| description | CD19+CD24hiCD38hi B cells are immature transitional B cells that, in normal individuals, exert suppressive effects by IL-10 production but are quantitatively altered and/or functionally impaired in individuals with various autoimmune diseases. Primary biliary cholangitis (PBC), an autoimmune disease, clinically presents as chronic cholestasis and nonsuppurative destructive cholangitis. A role for CD19+CD24hiCD38hi B cells in PBC is unknown. This study investigated the frequency and functional variation of circulating CD19+CD24hiCD38hi B cells in PBC patients. Flow cytometry was employed to quantify the percentage of CD19+CD24hiCD38hi B cells in peripheral blood samples. Correlations between CD19+CD24hiCD38hi B cells and routine laboratory parameters were assessed. Levels of IL-10, TNF-α, IL-6 and IL-12, and Tim-1 in CD19+CD24hiCD38hi B cells from PBC patients were analyzed. The effect of CD19+CD24hiCD38hi B cells on CD4+T cell differentiation was evaluated. The percentage of CD19+CD24hiCD38hi B cells in PBC patients was significantly higher than in healthy controls and was positively correlated with liver cholestasis. After activation by anti-B cell receptor and CpG, the production of IL-10 was decreased and the production of IL-6 and IL-12 was increased in CD19+CD24hiCD38hi B cells from PBC patients. Moreover, Tim-1 levels were significantly downregulated in CD19+CD24hiCD38hi B cells from PBC patients. Coculture showed that PBC-derived CD19+CD24hiCD38hi B cells were less capable of CD4+T cell inhibition, but promoted Th1 cell differentiation. In conclusion, PBC patients have expanded percentages, but impaired CD19+CD24hiCD38hi B cells, which correlate with disease damage. In PBC patients, this B cell subset has a skewed proinflammatory cytokine profile and a decreased capacity to suppress immune function, which may contribute to the pathogenesis of PBC. |
| format | Article |
| id | doaj-art-ea6acd8471434f8b9a7645caf4b23a28 |
| institution | DOAJ |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-ea6acd8471434f8b9a7645caf4b23a282025-08-20T03:20:39ZengWileyMediators of Inflammation0962-93511466-18612020-01-01202010.1155/2020/30193783019378CD19+CD24hiCD38hi B Cell Dysfunction in Primary Biliary CholangitisQubo Chen0Lanmin Lai1Xiaoling Chi2Xinyi Lu3Huaxian Wu4Jing Sun5Weilin Wu6Li Cai7Xuan Zeng8Chuyang Wang9WeiCheng Chen10Anping Peng11Biological Resource Center, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 510120 Guangzhou, ChinaBiological Resource Center, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 510120 Guangzhou, ChinaDivision of Hepatology, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 510120 Guangzhou, ChinaBiological Resource Center, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 510120 Guangzhou, ChinaDepartment of Laboratory Science, The Second Affiliated Hospital of Nanfang Medical University, 510120 Guangzhou, ChinaBiological Resource Center, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 510120 Guangzhou, ChinaBiological Resource Center, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 510120 Guangzhou, ChinaBiological Resource Center, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 510120 Guangzhou, ChinaBiological Resource Center, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 510120 Guangzhou, ChinaBiological Resource Center, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 510120 Guangzhou, ChinaBiological Resource Center, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 510120 Guangzhou, ChinaBiological Resource Center, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 510120 Guangzhou, ChinaCD19+CD24hiCD38hi B cells are immature transitional B cells that, in normal individuals, exert suppressive effects by IL-10 production but are quantitatively altered and/or functionally impaired in individuals with various autoimmune diseases. Primary biliary cholangitis (PBC), an autoimmune disease, clinically presents as chronic cholestasis and nonsuppurative destructive cholangitis. A role for CD19+CD24hiCD38hi B cells in PBC is unknown. This study investigated the frequency and functional variation of circulating CD19+CD24hiCD38hi B cells in PBC patients. Flow cytometry was employed to quantify the percentage of CD19+CD24hiCD38hi B cells in peripheral blood samples. Correlations between CD19+CD24hiCD38hi B cells and routine laboratory parameters were assessed. Levels of IL-10, TNF-α, IL-6 and IL-12, and Tim-1 in CD19+CD24hiCD38hi B cells from PBC patients were analyzed. The effect of CD19+CD24hiCD38hi B cells on CD4+T cell differentiation was evaluated. The percentage of CD19+CD24hiCD38hi B cells in PBC patients was significantly higher than in healthy controls and was positively correlated with liver cholestasis. After activation by anti-B cell receptor and CpG, the production of IL-10 was decreased and the production of IL-6 and IL-12 was increased in CD19+CD24hiCD38hi B cells from PBC patients. Moreover, Tim-1 levels were significantly downregulated in CD19+CD24hiCD38hi B cells from PBC patients. Coculture showed that PBC-derived CD19+CD24hiCD38hi B cells were less capable of CD4+T cell inhibition, but promoted Th1 cell differentiation. In conclusion, PBC patients have expanded percentages, but impaired CD19+CD24hiCD38hi B cells, which correlate with disease damage. In PBC patients, this B cell subset has a skewed proinflammatory cytokine profile and a decreased capacity to suppress immune function, which may contribute to the pathogenesis of PBC.http://dx.doi.org/10.1155/2020/3019378 |
| spellingShingle | Qubo Chen Lanmin Lai Xiaoling Chi Xinyi Lu Huaxian Wu Jing Sun Weilin Wu Li Cai Xuan Zeng Chuyang Wang WeiCheng Chen Anping Peng CD19+CD24hiCD38hi B Cell Dysfunction in Primary Biliary Cholangitis Mediators of Inflammation |
| title | CD19+CD24hiCD38hi B Cell Dysfunction in Primary Biliary Cholangitis |
| title_full | CD19+CD24hiCD38hi B Cell Dysfunction in Primary Biliary Cholangitis |
| title_fullStr | CD19+CD24hiCD38hi B Cell Dysfunction in Primary Biliary Cholangitis |
| title_full_unstemmed | CD19+CD24hiCD38hi B Cell Dysfunction in Primary Biliary Cholangitis |
| title_short | CD19+CD24hiCD38hi B Cell Dysfunction in Primary Biliary Cholangitis |
| title_sort | cd19 cd24hicd38hi b cell dysfunction in primary biliary cholangitis |
| url | http://dx.doi.org/10.1155/2020/3019378 |
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