Antarctic krill antioxidant peptides show inferior IgE-binding ability and RBL-2H3 cell degranulation
Enzymatic hydrolysis, isolation, and purification might make a great deal of difference in antioxidant activity and antigenicity of peptide components. This study aimed to isolate and purify antioxidant peptide components from Antarctic krill and evaluate their allergenicity of them. Electron parama...
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Tsinghua University Press
2023-09-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453023000289 |
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| author | Jie Ding Chunyan Zhu Pengfei Jiang Libo Qi Na Sun Songyi Lin |
| author_facet | Jie Ding Chunyan Zhu Pengfei Jiang Libo Qi Na Sun Songyi Lin |
| author_sort | Jie Ding |
| collection | DOAJ |
| description | Enzymatic hydrolysis, isolation, and purification might make a great deal of difference in antioxidant activity and antigenicity of peptide components. This study aimed to isolate and purify antioxidant peptide components from Antarctic krill and evaluate their allergenicity of them. Electron paramagnetic resonance (EPR) spectroscopy results indicated 310 kDa Antarctic krill hydrolysates (AKHs) had higher DPPH and ·OH radical scavenging rates. And the second component (N2-2) purified 310 kDa hydrolysate showed better ability to scavenge DPPH and ·OH radicals (P < 0.05), which were (47.43 ± 2.18)% and (34.33 ± 1.25)%, respectively. Additionally, indirect-ELISA results revealed that N2-1 had a weaker ability to bind specific IgE and that N2-2 had a lower binding capability to specific IgG1 (P < 0.05). And N2-2 had a higher EC50 value of (5.29 ± 0.95) ng/mL (P < 0.05) in cell degranulation assay, which was about 13.80 times that of Antarctic krill. Therefore, N2-2 might be the potential source of the antioxidant peptides with lower allergenicity. |
| format | Article |
| id | doaj-art-ea6a8b0b5ef44dd5ab07d91742c587a5 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-09-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-ea6a8b0b5ef44dd5ab07d91742c587a52025-02-03T00:27:37ZengTsinghua University PressFood Science and Human Wellness2213-45302023-09-0112517721778Antarctic krill antioxidant peptides show inferior IgE-binding ability and RBL-2H3 cell degranulationJie Ding0Chunyan Zhu1Pengfei Jiang2Libo Qi3Na Sun4Songyi Lin5National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China; Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, ChinaGanzhou Quanbiao Biological Technology Co., Ltd., Ganzhou 341100, ChinaNational Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China; Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, ChinaNational Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China; Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, ChinaNational Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China; Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, China; Corresponding authors at: National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China.National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China; Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, China; Corresponding authors at: National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China.Enzymatic hydrolysis, isolation, and purification might make a great deal of difference in antioxidant activity and antigenicity of peptide components. This study aimed to isolate and purify antioxidant peptide components from Antarctic krill and evaluate their allergenicity of them. Electron paramagnetic resonance (EPR) spectroscopy results indicated 310 kDa Antarctic krill hydrolysates (AKHs) had higher DPPH and ·OH radical scavenging rates. And the second component (N2-2) purified 310 kDa hydrolysate showed better ability to scavenge DPPH and ·OH radicals (P < 0.05), which were (47.43 ± 2.18)% and (34.33 ± 1.25)%, respectively. Additionally, indirect-ELISA results revealed that N2-1 had a weaker ability to bind specific IgE and that N2-2 had a lower binding capability to specific IgG1 (P < 0.05). And N2-2 had a higher EC50 value of (5.29 ± 0.95) ng/mL (P < 0.05) in cell degranulation assay, which was about 13.80 times that of Antarctic krill. Therefore, N2-2 might be the potential source of the antioxidant peptides with lower allergenicity.http://www.sciencedirect.com/science/article/pii/S2213453023000289Enzymatic hydrolysisIsolationPurificationAntarctic krill antioxidant peptideAllergenicity |
| spellingShingle | Jie Ding Chunyan Zhu Pengfei Jiang Libo Qi Na Sun Songyi Lin Antarctic krill antioxidant peptides show inferior IgE-binding ability and RBL-2H3 cell degranulation Food Science and Human Wellness Enzymatic hydrolysis Isolation Purification Antarctic krill antioxidant peptide Allergenicity |
| title | Antarctic krill antioxidant peptides show inferior IgE-binding ability and RBL-2H3 cell degranulation |
| title_full | Antarctic krill antioxidant peptides show inferior IgE-binding ability and RBL-2H3 cell degranulation |
| title_fullStr | Antarctic krill antioxidant peptides show inferior IgE-binding ability and RBL-2H3 cell degranulation |
| title_full_unstemmed | Antarctic krill antioxidant peptides show inferior IgE-binding ability and RBL-2H3 cell degranulation |
| title_short | Antarctic krill antioxidant peptides show inferior IgE-binding ability and RBL-2H3 cell degranulation |
| title_sort | antarctic krill antioxidant peptides show inferior ige binding ability and rbl 2h3 cell degranulation |
| topic | Enzymatic hydrolysis Isolation Purification Antarctic krill antioxidant peptide Allergenicity |
| url | http://www.sciencedirect.com/science/article/pii/S2213453023000289 |
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