Biofilm morphology and antibiotic susceptibility of methicillin-resistant Staphylococcus aureus (MRSA) on poly-D,L-lactide-co-poly(ethylene glycol) (PDLLA-PEG) coated titanium
Biodegradable polymeric coatings are being explored as a preventive strategy for orthopaedic device-related infection. In this study, titanium surfaces (Ti) were coated with poly-D,L-lactide (PDLLA, (P)), polyethylene-glycol poly-D,L-lactide (PEGylated-PDLLA, (PP20)), or multi-layered PEGylated-PDLL...
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Elsevier
2024-12-01
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| Series: | Biofilm |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590207524000534 |
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| author | Adam Benedict Turner David Zermeño-Pérez Margaritha M. Mysior Paula Milena Giraldo-Osorno Begoña García Elizabeth O'Gorman Shafik Oubihi Jeremy C. Simpson Iñigo Lasa Tadhg Ó Cróinín Margarita Trobos |
| author_facet | Adam Benedict Turner David Zermeño-Pérez Margaritha M. Mysior Paula Milena Giraldo-Osorno Begoña García Elizabeth O'Gorman Shafik Oubihi Jeremy C. Simpson Iñigo Lasa Tadhg Ó Cróinín Margarita Trobos |
| author_sort | Adam Benedict Turner |
| collection | DOAJ |
| description | Biodegradable polymeric coatings are being explored as a preventive strategy for orthopaedic device-related infection. In this study, titanium surfaces (Ti) were coated with poly-D,L-lactide (PDLLA, (P)), polyethylene-glycol poly-D,L-lactide (PEGylated-PDLLA, (PP20)), or multi-layered PEGylated-PDLLA (M), with or without 1 % silver sulfadiazine. The aim was to evaluate their cytocompatibility, resistance to Staphylococcus aureus biofilm formation, and their potential to enhance the susceptibility of any biofilm formed to antibiotics. Using automated high-content screening confocal microscopy, biofilm formation of a clinical methicillin-resistant Staphylococcus aureus (MRSA) isolate expressing GFP was quantified, along with isogenic mutants that were unable to form polysaccharidic or proteinaceous biofilm matrices. The results showed that PEGylated-PDLLA coatings exhibited significant antibiofilm properties, with M showing the highest effect. This inhibitory effect was stronger in S. aureus biofilms with a matrix composed of proteins compared to those with an exopolysaccharide (PIA) biofilm matrix. Our data suggest that the antibiofilm effect may have been due to (i) inhibition of the initial attachment through microbial surface components recognising adhesive matrix molecules (MSCRAMMs), since PEG reduces protein surface adsorption via surface hydration layer and steric repulsion; and (ii) mechanical disaggregation and dispersal of microcolonies due to the bioresorbable/degradable nature of the polymers, which undergo hydration and hydrolysis over time. The disruption of biofilm morphology by the PDLLA-PEG co-polymers increased S. aureus susceptibility to antibiotics like rifampicin and fusidic acid. Adding 1 % AgSD provided additional early bactericidal effects on both biofilm and planktonic S. aureus. Additionally, the coatings were cytocompatible with immune cells, indicating their potential to enhance bacterial clearance and reduce bacterial colonisation of titanium-based orthopaedic biomaterials. |
| format | Article |
| id | doaj-art-ea68b7515b7740c886abc50ab4eb376c |
| institution | DOAJ |
| issn | 2590-2075 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biofilm |
| spelling | doaj-art-ea68b7515b7740c886abc50ab4eb376c2025-08-20T02:50:26ZengElsevierBiofilm2590-20752024-12-01810022810.1016/j.bioflm.2024.100228Biofilm morphology and antibiotic susceptibility of methicillin-resistant Staphylococcus aureus (MRSA) on poly-D,L-lactide-co-poly(ethylene glycol) (PDLLA-PEG) coated titaniumAdam Benedict Turner0David Zermeño-Pérez1Margaritha M. Mysior2Paula Milena Giraldo-Osorno3Begoña García4Elizabeth O'Gorman5Shafik Oubihi6Jeremy C. Simpson7Iñigo Lasa8Tadhg Ó Cróinín9Margarita Trobos10Department of Biomaterials, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden; Centre for Antibiotic Resistance Research in Gothenburg (CARe), Gothenburg, SwedenAshland Specialties Ireland Ltd., Mullingar, Ireland; School of Biomolecular and Biomedical Science, University College Dublin, Dublin, IrelandCell Screening Laboratory, UCD School of Biology & Environmental Science, University College Dublin, Dublin, IrelandDepartment of Biomaterials, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden; Centre for Antibiotic Resistance Research in Gothenburg (CARe), Gothenburg, SwedenMicrobial Pathogenesis Laboratory. Navarrabiomed-Complejo Hospitalario de Navarra (CHN)-Universidad Pública de Navarra (UPNA), IDISNA, Pamplona, Navarra, SpainSchool of Biomolecular and Biomedical Science, University College Dublin, Dublin, IrelandSchool of Biomolecular and Biomedical Science, University College Dublin, Dublin, IrelandCell Screening Laboratory, UCD School of Biology & Environmental Science, University College Dublin, Dublin, IrelandMicrobial Pathogenesis Laboratory. Navarrabiomed-Complejo Hospitalario de Navarra (CHN)-Universidad Pública de Navarra (UPNA), IDISNA, Pamplona, Navarra, SpainSchool of Biomolecular and Biomedical Science, University College Dublin, Dublin, Ireland; Corresponding author. UCD School of Biomolecular and Biomedical Science, Room C140, Health Science Centre, Belfield, Dublin, Ireland.Department of Biomaterials, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden; Centre for Antibiotic Resistance Research in Gothenburg (CARe), Gothenburg, Sweden; Corresponding author. Department of Biomaterials, Institute of Clinial Sciences, Sahlgrenska Academy, University of Gothenburg, P.O. Box 412, 405 30 Gothenburg, Sweden.Biodegradable polymeric coatings are being explored as a preventive strategy for orthopaedic device-related infection. In this study, titanium surfaces (Ti) were coated with poly-D,L-lactide (PDLLA, (P)), polyethylene-glycol poly-D,L-lactide (PEGylated-PDLLA, (PP20)), or multi-layered PEGylated-PDLLA (M), with or without 1 % silver sulfadiazine. The aim was to evaluate their cytocompatibility, resistance to Staphylococcus aureus biofilm formation, and their potential to enhance the susceptibility of any biofilm formed to antibiotics. Using automated high-content screening confocal microscopy, biofilm formation of a clinical methicillin-resistant Staphylococcus aureus (MRSA) isolate expressing GFP was quantified, along with isogenic mutants that were unable to form polysaccharidic or proteinaceous biofilm matrices. The results showed that PEGylated-PDLLA coatings exhibited significant antibiofilm properties, with M showing the highest effect. This inhibitory effect was stronger in S. aureus biofilms with a matrix composed of proteins compared to those with an exopolysaccharide (PIA) biofilm matrix. Our data suggest that the antibiofilm effect may have been due to (i) inhibition of the initial attachment through microbial surface components recognising adhesive matrix molecules (MSCRAMMs), since PEG reduces protein surface adsorption via surface hydration layer and steric repulsion; and (ii) mechanical disaggregation and dispersal of microcolonies due to the bioresorbable/degradable nature of the polymers, which undergo hydration and hydrolysis over time. The disruption of biofilm morphology by the PDLLA-PEG co-polymers increased S. aureus susceptibility to antibiotics like rifampicin and fusidic acid. Adding 1 % AgSD provided additional early bactericidal effects on both biofilm and planktonic S. aureus. Additionally, the coatings were cytocompatible with immune cells, indicating their potential to enhance bacterial clearance and reduce bacterial colonisation of titanium-based orthopaedic biomaterials.http://www.sciencedirect.com/science/article/pii/S2590207524000534Poly-D,L-lactide (PDLLA)Polyethylene-glycol (PEG)biofilmMethicillin-resistant Staphylococcus aureus (MRSA)Antibiotics |
| spellingShingle | Adam Benedict Turner David Zermeño-Pérez Margaritha M. Mysior Paula Milena Giraldo-Osorno Begoña García Elizabeth O'Gorman Shafik Oubihi Jeremy C. Simpson Iñigo Lasa Tadhg Ó Cróinín Margarita Trobos Biofilm morphology and antibiotic susceptibility of methicillin-resistant Staphylococcus aureus (MRSA) on poly-D,L-lactide-co-poly(ethylene glycol) (PDLLA-PEG) coated titanium Biofilm Poly-D,L-lactide (PDLLA) Polyethylene-glycol (PEG) biofilm Methicillin-resistant Staphylococcus aureus (MRSA) Antibiotics |
| title | Biofilm morphology and antibiotic susceptibility of methicillin-resistant Staphylococcus aureus (MRSA) on poly-D,L-lactide-co-poly(ethylene glycol) (PDLLA-PEG) coated titanium |
| title_full | Biofilm morphology and antibiotic susceptibility of methicillin-resistant Staphylococcus aureus (MRSA) on poly-D,L-lactide-co-poly(ethylene glycol) (PDLLA-PEG) coated titanium |
| title_fullStr | Biofilm morphology and antibiotic susceptibility of methicillin-resistant Staphylococcus aureus (MRSA) on poly-D,L-lactide-co-poly(ethylene glycol) (PDLLA-PEG) coated titanium |
| title_full_unstemmed | Biofilm morphology and antibiotic susceptibility of methicillin-resistant Staphylococcus aureus (MRSA) on poly-D,L-lactide-co-poly(ethylene glycol) (PDLLA-PEG) coated titanium |
| title_short | Biofilm morphology and antibiotic susceptibility of methicillin-resistant Staphylococcus aureus (MRSA) on poly-D,L-lactide-co-poly(ethylene glycol) (PDLLA-PEG) coated titanium |
| title_sort | biofilm morphology and antibiotic susceptibility of methicillin resistant staphylococcus aureus mrsa on poly d l lactide co poly ethylene glycol pdlla peg coated titanium |
| topic | Poly-D,L-lactide (PDLLA) Polyethylene-glycol (PEG) biofilm Methicillin-resistant Staphylococcus aureus (MRSA) Antibiotics |
| url | http://www.sciencedirect.com/science/article/pii/S2590207524000534 |
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