FGF7 secreted from dermal papillae cell regulates the proliferation and differentiation of hair follicle stem cell
Hair follicle stem cell (HFSC), capable of self-renewal and differentiation in hair follicle, represents an emerging stem cell model for regenerative medicine. The interaction between HFSC and dermal papilla cell (DPC) governs hair follicle development. FGF7 functions as a paracrine protein regulati...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2025-09-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311923003544 |
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| author | Niu Wang Weidong Zhang Zhenyu Zhong Xiongbo Zhou Xinran Shi Xin Wang |
| author_facet | Niu Wang Weidong Zhang Zhenyu Zhong Xiongbo Zhou Xinran Shi Xin Wang |
| author_sort | Niu Wang |
| collection | DOAJ |
| description | Hair follicle stem cell (HFSC), capable of self-renewal and differentiation in hair follicle, represents an emerging stem cell model for regenerative medicine. The interaction between HFSC and dermal papilla cell (DPC) governs hair follicle development. FGF7 functions as a paracrine protein regulating epithelial proliferation, differentiation and migration. The single-cell transcriptome profiling and immunofluorescence analysis demonstrated that FGF7 localizes at DPC, while FGF7 receptor (FGFR2) expresses in both DPC and HFSC. Through co-culture experiments of HFSC and DPC, the results indicated that FGF7 secreted from DPC promotes the proliferation of DPC and HFSC via Wnt signaling pathway and induces HFSC differentiation. Furthermore, CUT&Tag assay revealed genomic colocalization between FGF7 and pluripotency-related genes and GSK3β. Electrophoretic mobility shift assay (EMSA) demonstrated that FGF7 interacts with the promoter region of CISH and PRKX. This research provides valuable insights into the molecular mechanisms underlying the hair cycle. Understanding the interaction between HFSC and DPC, as well as the role of FGF7, may advance regenerative medicine and hair loss treatment. |
| format | Article |
| id | doaj-art-ea671c2682604f93b6ee80ebe00c761c |
| institution | Kabale University |
| issn | 2095-3119 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-ea671c2682604f93b6ee80ebe00c761c2025-08-21T04:16:34ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192025-09-012493583359710.1016/j.jia.2023.10.012FGF7 secreted from dermal papillae cell regulates the proliferation and differentiation of hair follicle stem cellNiu Wang0Weidong Zhang1Zhenyu Zhong2Xiongbo Zhou3Xinran Shi4Xin Wang5College of Animal Science and Technology, Northwest A&F University, Yangling 712100, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling 712100, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling 712100, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling 712100, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling 712100, ChinaCorrespondence Xin Wang; College of Animal Science and Technology, Northwest A&F University, Yangling 712100, ChinaHair follicle stem cell (HFSC), capable of self-renewal and differentiation in hair follicle, represents an emerging stem cell model for regenerative medicine. The interaction between HFSC and dermal papilla cell (DPC) governs hair follicle development. FGF7 functions as a paracrine protein regulating epithelial proliferation, differentiation and migration. The single-cell transcriptome profiling and immunofluorescence analysis demonstrated that FGF7 localizes at DPC, while FGF7 receptor (FGFR2) expresses in both DPC and HFSC. Through co-culture experiments of HFSC and DPC, the results indicated that FGF7 secreted from DPC promotes the proliferation of DPC and HFSC via Wnt signaling pathway and induces HFSC differentiation. Furthermore, CUT&Tag assay revealed genomic colocalization between FGF7 and pluripotency-related genes and GSK3β. Electrophoretic mobility shift assay (EMSA) demonstrated that FGF7 interacts with the promoter region of CISH and PRKX. This research provides valuable insights into the molecular mechanisms underlying the hair cycle. Understanding the interaction between HFSC and DPC, as well as the role of FGF7, may advance regenerative medicine and hair loss treatment.http://www.sciencedirect.com/science/article/pii/S2095311923003544FGF7dermal papilla cellhair follicle stem cellproliferationdifferentiationCUT&Tag assay |
| spellingShingle | Niu Wang Weidong Zhang Zhenyu Zhong Xiongbo Zhou Xinran Shi Xin Wang FGF7 secreted from dermal papillae cell regulates the proliferation and differentiation of hair follicle stem cell Journal of Integrative Agriculture FGF7 dermal papilla cell hair follicle stem cell proliferation differentiation CUT&Tag assay |
| title | FGF7 secreted from dermal papillae cell regulates the proliferation and differentiation of hair follicle stem cell |
| title_full | FGF7 secreted from dermal papillae cell regulates the proliferation and differentiation of hair follicle stem cell |
| title_fullStr | FGF7 secreted from dermal papillae cell regulates the proliferation and differentiation of hair follicle stem cell |
| title_full_unstemmed | FGF7 secreted from dermal papillae cell regulates the proliferation and differentiation of hair follicle stem cell |
| title_short | FGF7 secreted from dermal papillae cell regulates the proliferation and differentiation of hair follicle stem cell |
| title_sort | fgf7 secreted from dermal papillae cell regulates the proliferation and differentiation of hair follicle stem cell |
| topic | FGF7 dermal papilla cell hair follicle stem cell proliferation differentiation CUT&Tag assay |
| url | http://www.sciencedirect.com/science/article/pii/S2095311923003544 |
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