Local Application of a New Chalconic Derivative (Chalcone T4) Reduces Inflammation and Oxidative Stress in a Periodontitis Model in Rats
Chalcones are phenolic compounds with biological properties. This study had the aim to evaluate the effects of topical administration of a new synthetic chalcone, Chalcone T4, in an animal model of periodontitis induced by ligature. Forty rats were distributed in the following experimental groups: n...
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MDPI AG
2024-09-01
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| Series: | Antioxidants |
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| author | Angelo Constantino Camilli Mariely Araújo de Godoi Vitória Bonan Costa Natalie Aparecida Rodrigues Fernandes Giovani Cirelli Larissa Kely Faustino da Silva Letícia Ribeiro Assis Luis Octavio Regasini Morgana Rodrigues Guimarães-Stabili |
| author_facet | Angelo Constantino Camilli Mariely Araújo de Godoi Vitória Bonan Costa Natalie Aparecida Rodrigues Fernandes Giovani Cirelli Larissa Kely Faustino da Silva Letícia Ribeiro Assis Luis Octavio Regasini Morgana Rodrigues Guimarães-Stabili |
| author_sort | Angelo Constantino Camilli |
| collection | DOAJ |
| description | Chalcones are phenolic compounds with biological properties. This study had the aim to evaluate the effects of topical administration of a new synthetic chalcone, Chalcone T4, in an animal model of periodontitis induced by ligature. Forty rats were distributed in the following experimental groups: negative control (without periodontitis and topical application of distilled water), positive control (periodontitis and topical application of distilled water), chalcone I and II (periodontitis and topical application of 0.6 mg/mL and 1.8 mg/mL, respectively). Chalcone or distilled water was administered into the gingival sulcus of the first molars daily for 10 days, starting with the ligature installation. The following outcomes were evaluated: alveolar bone loss (µCT and methylene blue dye staining), quantification of osteoclasts (histomorphometry), cell infiltrate and collagen content (stereometry), gene expression of mediators (<i>Nfact11, Tnf-α, Mmp-13, iNos, Sod and Nrf2</i>) by (RT-qPCR); expression of BCL-2 and Caspase-1 (immunohistochemistry). Chalcone T4 inhibited bone resorption and prevented collagen matrix degradation. Reduction in the expression of inflammatory markers (<i>Nfact11, Tnf-α, Mmp-13</i>, and Caspase-1), attenuation of oxidative stress (<i>iNOS</i> reduction, and increase in <i>Sod</i>), and pro-apoptotic effect of the compound (BCL-2 reduction), were associated its effects on periodontal tissues. Topical application of Chalcone T4 prevented bone resorption and inflammation, demonstrating potential in the adjunctive treatment of periodontitis. |
| format | Article |
| id | doaj-art-ea6352d1b2754c879f5417eb50a685f7 |
| institution | OA Journals |
| issn | 2076-3921 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | MDPI AG |
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| series | Antioxidants |
| spelling | doaj-art-ea6352d1b2754c879f5417eb50a685f72025-08-20T02:11:08ZengMDPI AGAntioxidants2076-39212024-09-011310119210.3390/antiox13101192Local Application of a New Chalconic Derivative (Chalcone T4) Reduces Inflammation and Oxidative Stress in a Periodontitis Model in RatsAngelo Constantino Camilli0Mariely Araújo de Godoi1Vitória Bonan Costa2Natalie Aparecida Rodrigues Fernandes3Giovani Cirelli4Larissa Kely Faustino da Silva5Letícia Ribeiro Assis6Luis Octavio Regasini7Morgana Rodrigues Guimarães-Stabili8Department of Diagnosis and Surgery, School of Dentistry at Araraquara, São Paulo State University (UNESP), Araraquara 14801-903, SP, BrazilDepartment of Diagnosis and Surgery, School of Dentistry at Araraquara, São Paulo State University (UNESP), Araraquara 14801-903, SP, BrazilDepartment of Diagnosis and Surgery, School of Dentistry at Araraquara, São Paulo State University (UNESP), Araraquara 14801-903, SP, BrazilDepartment of Diagnosis and Surgery, School of Dentistry at Araraquara, São Paulo State University (UNESP), Araraquara 14801-903, SP, BrazilDepartment of Diagnosis and Surgery, School of Dentistry at Araraquara, São Paulo State University (UNESP), Araraquara 14801-903, SP, BrazilDepartment of Diagnosis and Surgery, School of Dentistry at Araraquara, São Paulo State University (UNESP), Araraquara 14801-903, SP, BrazilDepartment of Chemistry and Environmental Sciences, Institute of Biosciences, Humanities and Exact Sciences, São Paulo State University (UNESP), São José do Rio Preto 01049-010, SP, BrazilDepartment of Chemistry and Environmental Sciences, Institute of Biosciences, Humanities and Exact Sciences, São Paulo State University (UNESP), São José do Rio Preto 01049-010, SP, BrazilDepartment of Diagnosis and Surgery, School of Dentistry at Araraquara, São Paulo State University (UNESP), Araraquara 14801-903, SP, BrazilChalcones are phenolic compounds with biological properties. This study had the aim to evaluate the effects of topical administration of a new synthetic chalcone, Chalcone T4, in an animal model of periodontitis induced by ligature. Forty rats were distributed in the following experimental groups: negative control (without periodontitis and topical application of distilled water), positive control (periodontitis and topical application of distilled water), chalcone I and II (periodontitis and topical application of 0.6 mg/mL and 1.8 mg/mL, respectively). Chalcone or distilled water was administered into the gingival sulcus of the first molars daily for 10 days, starting with the ligature installation. The following outcomes were evaluated: alveolar bone loss (µCT and methylene blue dye staining), quantification of osteoclasts (histomorphometry), cell infiltrate and collagen content (stereometry), gene expression of mediators (<i>Nfact11, Tnf-α, Mmp-13, iNos, Sod and Nrf2</i>) by (RT-qPCR); expression of BCL-2 and Caspase-1 (immunohistochemistry). Chalcone T4 inhibited bone resorption and prevented collagen matrix degradation. Reduction in the expression of inflammatory markers (<i>Nfact11, Tnf-α, Mmp-13</i>, and Caspase-1), attenuation of oxidative stress (<i>iNOS</i> reduction, and increase in <i>Sod</i>), and pro-apoptotic effect of the compound (BCL-2 reduction), were associated its effects on periodontal tissues. Topical application of Chalcone T4 prevented bone resorption and inflammation, demonstrating potential in the adjunctive treatment of periodontitis.https://www.mdpi.com/2076-3921/13/10/1192periodontitischalconetopical applicationantioxidant activity |
| spellingShingle | Angelo Constantino Camilli Mariely Araújo de Godoi Vitória Bonan Costa Natalie Aparecida Rodrigues Fernandes Giovani Cirelli Larissa Kely Faustino da Silva Letícia Ribeiro Assis Luis Octavio Regasini Morgana Rodrigues Guimarães-Stabili Local Application of a New Chalconic Derivative (Chalcone T4) Reduces Inflammation and Oxidative Stress in a Periodontitis Model in Rats Antioxidants periodontitis chalcone topical application antioxidant activity |
| title | Local Application of a New Chalconic Derivative (Chalcone T4) Reduces Inflammation and Oxidative Stress in a Periodontitis Model in Rats |
| title_full | Local Application of a New Chalconic Derivative (Chalcone T4) Reduces Inflammation and Oxidative Stress in a Periodontitis Model in Rats |
| title_fullStr | Local Application of a New Chalconic Derivative (Chalcone T4) Reduces Inflammation and Oxidative Stress in a Periodontitis Model in Rats |
| title_full_unstemmed | Local Application of a New Chalconic Derivative (Chalcone T4) Reduces Inflammation and Oxidative Stress in a Periodontitis Model in Rats |
| title_short | Local Application of a New Chalconic Derivative (Chalcone T4) Reduces Inflammation and Oxidative Stress in a Periodontitis Model in Rats |
| title_sort | local application of a new chalconic derivative chalcone t4 reduces inflammation and oxidative stress in a periodontitis model in rats |
| topic | periodontitis chalcone topical application antioxidant activity |
| url | https://www.mdpi.com/2076-3921/13/10/1192 |
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