Aspirin increases metabolism through germline signalling to extend the lifespan of Caenorhabditis elegans.
Aspirin is a prototypic cyclooxygenase inhibitor with a variety of beneficial effects on human health. It prevents age-related diseases and delays the aging process. Previous research has shown that aspirin might act through a dietary restriction-like mechanism to extend lifespan. To explore the mec...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0184027&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850076429325172736 |
|---|---|
| author | Xiao-Bing Huang Xiao-Hui Mu Qin-Li Wan Xiao-Ming He Gui-Sheng Wu Huai-Rong Luo |
| author_facet | Xiao-Bing Huang Xiao-Hui Mu Qin-Li Wan Xiao-Ming He Gui-Sheng Wu Huai-Rong Luo |
| author_sort | Xiao-Bing Huang |
| collection | DOAJ |
| description | Aspirin is a prototypic cyclooxygenase inhibitor with a variety of beneficial effects on human health. It prevents age-related diseases and delays the aging process. Previous research has shown that aspirin might act through a dietary restriction-like mechanism to extend lifespan. To explore the mechanism of action of aspirin on aging, we determined the whole-genome expression profile of Caenorhabditis elegans treated with aspirin. Transcriptome analysis revealed the RNA levels of genes involved in metabolism were primarily increased. Reproduction has been reported to be associated with metabolism. We found that aspirin did not extend the lifespan or improve the heat stress resistance of germline mutants of glp-1. Furthermore, Oil Red O staining showed that aspirin treatment decreased lipid deposition and increased expression of lipid hydrolysis and fatty acid β-oxidation-related genes. The effect of germline ablation on lifespan was mainly mediated by DAF-12 and DAF-16. Next, we performed genetic analysis with a series of worm mutants and found that aspirin did not further extend the lifespans of daf-12 and daf-16 single mutants, glp-1;daf-12 and glp-1;daf-16 double mutants, or glp-1;daf-12;daf-16 triple mutants. The results suggest that aspirin increase metabolism and regulate germline signalling to activate downstream DAF-12 and DAF-16 to extend lifespan. |
| format | Article |
| id | doaj-art-ea5d40a1ce3440d2a53446e706eeac4e |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-ea5d40a1ce3440d2a53446e706eeac4e2025-08-20T02:46:01ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01129e018402710.1371/journal.pone.0184027Aspirin increases metabolism through germline signalling to extend the lifespan of Caenorhabditis elegans.Xiao-Bing HuangXiao-Hui MuQin-Li WanXiao-Ming HeGui-Sheng WuHuai-Rong LuoAspirin is a prototypic cyclooxygenase inhibitor with a variety of beneficial effects on human health. It prevents age-related diseases and delays the aging process. Previous research has shown that aspirin might act through a dietary restriction-like mechanism to extend lifespan. To explore the mechanism of action of aspirin on aging, we determined the whole-genome expression profile of Caenorhabditis elegans treated with aspirin. Transcriptome analysis revealed the RNA levels of genes involved in metabolism were primarily increased. Reproduction has been reported to be associated with metabolism. We found that aspirin did not extend the lifespan or improve the heat stress resistance of germline mutants of glp-1. Furthermore, Oil Red O staining showed that aspirin treatment decreased lipid deposition and increased expression of lipid hydrolysis and fatty acid β-oxidation-related genes. The effect of germline ablation on lifespan was mainly mediated by DAF-12 and DAF-16. Next, we performed genetic analysis with a series of worm mutants and found that aspirin did not further extend the lifespans of daf-12 and daf-16 single mutants, glp-1;daf-12 and glp-1;daf-16 double mutants, or glp-1;daf-12;daf-16 triple mutants. The results suggest that aspirin increase metabolism and regulate germline signalling to activate downstream DAF-12 and DAF-16 to extend lifespan.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0184027&type=printable |
| spellingShingle | Xiao-Bing Huang Xiao-Hui Mu Qin-Li Wan Xiao-Ming He Gui-Sheng Wu Huai-Rong Luo Aspirin increases metabolism through germline signalling to extend the lifespan of Caenorhabditis elegans. PLoS ONE |
| title | Aspirin increases metabolism through germline signalling to extend the lifespan of Caenorhabditis elegans. |
| title_full | Aspirin increases metabolism through germline signalling to extend the lifespan of Caenorhabditis elegans. |
| title_fullStr | Aspirin increases metabolism through germline signalling to extend the lifespan of Caenorhabditis elegans. |
| title_full_unstemmed | Aspirin increases metabolism through germline signalling to extend the lifespan of Caenorhabditis elegans. |
| title_short | Aspirin increases metabolism through germline signalling to extend the lifespan of Caenorhabditis elegans. |
| title_sort | aspirin increases metabolism through germline signalling to extend the lifespan of caenorhabditis elegans |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0184027&type=printable |
| work_keys_str_mv | AT xiaobinghuang aspirinincreasesmetabolismthroughgermlinesignallingtoextendthelifespanofcaenorhabditiselegans AT xiaohuimu aspirinincreasesmetabolismthroughgermlinesignallingtoextendthelifespanofcaenorhabditiselegans AT qinliwan aspirinincreasesmetabolismthroughgermlinesignallingtoextendthelifespanofcaenorhabditiselegans AT xiaominghe aspirinincreasesmetabolismthroughgermlinesignallingtoextendthelifespanofcaenorhabditiselegans AT guishengwu aspirinincreasesmetabolismthroughgermlinesignallingtoextendthelifespanofcaenorhabditiselegans AT huairongluo aspirinincreasesmetabolismthroughgermlinesignallingtoextendthelifespanofcaenorhabditiselegans |