Association between Genetic Variants in DUSP15, CNTNAP2, and PCDHA Genes and Risk of Childhood Autism Spectrum Disorder

Objective. Genetic factors play an important role in the development of autism spectrum disorder (ASD). This case-control study was to determine the association between childhood ASD and single nucleotide polymorphisms (SNPs) rs3746599 in the DUSP15 gene, rs7794745 in the CNTNAP2 gene, and rs251379...

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Main Authors: Fang Fang, Minxia Ge, Jun Liu, Zengyu Zhang, Hong Yu, Shuilong Zhu, Liwei Xu, Lina Shao
Format: Article
Language:English
Published: Wiley 2021-01-01
Series:Behavioural Neurology
Online Access:http://dx.doi.org/10.1155/2021/4150926
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author Fang Fang
Minxia Ge
Jun Liu
Zengyu Zhang
Hong Yu
Shuilong Zhu
Liwei Xu
Lina Shao
author_facet Fang Fang
Minxia Ge
Jun Liu
Zengyu Zhang
Hong Yu
Shuilong Zhu
Liwei Xu
Lina Shao
author_sort Fang Fang
collection DOAJ
description Objective. Genetic factors play an important role in the development of autism spectrum disorder (ASD). This case-control study was to determine the association between childhood ASD and single nucleotide polymorphisms (SNPs) rs3746599 in the DUSP15 gene, rs7794745 in the CNTNAP2 gene, and rs251379 in the PCDHA gene in a Chinese Han population. Methods. Genotypes of SNPs were examined in DNA extracted from blood cells from 201 children with ASD and 200 healthy controls. The Children Autism Rating Scale (CARS) was applied to evaluate the severity of the disease and language impairment. The relationship between SNPs and the risk of ASD or the severity of the disease was determined by logistic regression and one-way ANOVA. Results. The genotype G/G of rs3746599 in the DUSP15 gene was significantly associated with a decreased risk of ASD (odds ratio OR=0.65, 95% confidence interval (CI): 0.42-0.99, P=0.0449). The T allele of rs7794745 in the CNTNAP2 gene was associated with an increased risk of ASD (OR=1.34, 95% CI: 1.01-1.77, P=0.0435). The SNP rs251379 was not associated with ASD. Though none of the SNPs examined were associated with ASD severity, rs7794745 was associated with severity of language impairment. Conclusions. Our findings suggest that both rs3746599 in the DUSP15 gene and rs7794745 in the CNTNAP2 gene are associated with risk of childhood ASD, and rs7794745 is also related to the severity of language impairment in autistic children from a Chinese Han population.
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spelling doaj-art-ea5af44f7a774f2bbf08304a0fd22a052025-08-20T03:20:39ZengWileyBehavioural Neurology0953-41801875-85842021-01-01202110.1155/2021/41509264150926Association between Genetic Variants in DUSP15, CNTNAP2, and PCDHA Genes and Risk of Childhood Autism Spectrum DisorderFang Fang0Minxia Ge1Jun Liu2Zengyu Zhang3Hong Yu4Shuilong Zhu5Liwei Xu6Lina Shao7Department of Clinical Laboratory, The Third People’s Hospital of Xiaoshan District, Hangzhou, ChinaDepartment of Clinical Laboratory, The Third People’s Hospital of Xiaoshan District, Hangzhou, ChinaCentral Laboratory, Department of Clinical Laboratory, Zhejiang Xiaoshan Hospital, Hangzhou, ChinaDepartment of Pediatrics, Xiaoshan First Affiliated Hospital of Hangzhou Normal University, Hangzhou, ChinaDepartment of Clinical Psychology, Xiaoshan First Affiliated Hospital of Hangzhou Normal University, Hangzhou, ChinaDepartment of Clinical Laboratory, The Third People’s Hospital of Xiaoshan District, Hangzhou, ChinaDepartment of Clinical Laboratory, The Third People’s Hospital of Xiaoshan District, Hangzhou, ChinaDepartment of Clinical Laboratory, The Third People’s Hospital of Xiaoshan District, Hangzhou, ChinaObjective. Genetic factors play an important role in the development of autism spectrum disorder (ASD). This case-control study was to determine the association between childhood ASD and single nucleotide polymorphisms (SNPs) rs3746599 in the DUSP15 gene, rs7794745 in the CNTNAP2 gene, and rs251379 in the PCDHA gene in a Chinese Han population. Methods. Genotypes of SNPs were examined in DNA extracted from blood cells from 201 children with ASD and 200 healthy controls. The Children Autism Rating Scale (CARS) was applied to evaluate the severity of the disease and language impairment. The relationship between SNPs and the risk of ASD or the severity of the disease was determined by logistic regression and one-way ANOVA. Results. The genotype G/G of rs3746599 in the DUSP15 gene was significantly associated with a decreased risk of ASD (odds ratio OR=0.65, 95% confidence interval (CI): 0.42-0.99, P=0.0449). The T allele of rs7794745 in the CNTNAP2 gene was associated with an increased risk of ASD (OR=1.34, 95% CI: 1.01-1.77, P=0.0435). The SNP rs251379 was not associated with ASD. Though none of the SNPs examined were associated with ASD severity, rs7794745 was associated with severity of language impairment. Conclusions. Our findings suggest that both rs3746599 in the DUSP15 gene and rs7794745 in the CNTNAP2 gene are associated with risk of childhood ASD, and rs7794745 is also related to the severity of language impairment in autistic children from a Chinese Han population.http://dx.doi.org/10.1155/2021/4150926
spellingShingle Fang Fang
Minxia Ge
Jun Liu
Zengyu Zhang
Hong Yu
Shuilong Zhu
Liwei Xu
Lina Shao
Association between Genetic Variants in DUSP15, CNTNAP2, and PCDHA Genes and Risk of Childhood Autism Spectrum Disorder
Behavioural Neurology
title Association between Genetic Variants in DUSP15, CNTNAP2, and PCDHA Genes and Risk of Childhood Autism Spectrum Disorder
title_full Association between Genetic Variants in DUSP15, CNTNAP2, and PCDHA Genes and Risk of Childhood Autism Spectrum Disorder
title_fullStr Association between Genetic Variants in DUSP15, CNTNAP2, and PCDHA Genes and Risk of Childhood Autism Spectrum Disorder
title_full_unstemmed Association between Genetic Variants in DUSP15, CNTNAP2, and PCDHA Genes and Risk of Childhood Autism Spectrum Disorder
title_short Association between Genetic Variants in DUSP15, CNTNAP2, and PCDHA Genes and Risk of Childhood Autism Spectrum Disorder
title_sort association between genetic variants in dusp15 cntnap2 and pcdha genes and risk of childhood autism spectrum disorder
url http://dx.doi.org/10.1155/2021/4150926
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