The effect of a water-soluble β-glucan on intestinal immunity and microbiota in LPS-challenged piglets
The intestine is the largest immune and barrier organ in the body, and diarrhea and even death during piglet development are related to dysfunction caused by intestinal barrier damage and inflammation. A water-soluble β-glucan produced by Agrobacterium ZX09 has been shown to have a beneficial effect...
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Frontiers Media S.A.
2025-03-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2025.1533872/full |
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| author | Yuliang Wu Yuliang Wu Yuxin Li Yuxin Li Mengli Chen Mengli Chen Juan Zhao Xia Xiong Xia Xiong Xia Xiong Chen Guang Olnood Yundi Gao Fei Wang Can Peng Can Peng Miao Liu Miao Liu Chunxia Huang Jianzhong Li Liuqin He Huansheng Yang Yulong Yin Yulong Yin Yulong Yin |
| author_facet | Yuliang Wu Yuliang Wu Yuxin Li Yuxin Li Mengli Chen Mengli Chen Juan Zhao Xia Xiong Xia Xiong Xia Xiong Chen Guang Olnood Yundi Gao Fei Wang Can Peng Can Peng Miao Liu Miao Liu Chunxia Huang Jianzhong Li Liuqin He Huansheng Yang Yulong Yin Yulong Yin Yulong Yin |
| author_sort | Yuliang Wu |
| collection | DOAJ |
| description | The intestine is the largest immune and barrier organ in the body, and diarrhea and even death during piglet development are related to dysfunction caused by intestinal barrier damage and inflammation. A water-soluble β-glucan produced by Agrobacterium ZX09 has been shown to have a beneficial effect on gastrointestinal health. The main objective of this study was to investigate whether pre-feeding β-glucan has a protective effect on LPS-induced immune stress in piglets. In this study, 24 weaned piglets (21-day-old; 6.64 ± 0.16 kg) were assigned to 4 treatments in a two × two factorial design with diet (with or without β-glucan) and immunological challenge (saline or LPS). Piglets were challenged with saline or LPS after 39 days of feeding 0 or 200 mg/kg β-glucan. The results demonstrated that β-glucan supplementation increased the average daily weight gain and daily feed intake, and decreased diarrhea rate of piglets. Intestinal inflammation symptoms and histological changes in LPS-challenged piglets were alleviated by pre-feeding of β-glucan. β-glucan supplementation reduced serum IL-1β (interleukin-1β) and NO (nitric oxide) secretion in piglets after LPS challenge (0.01 < p < 0.05). Supplementation with β-glucan downregulated the mRNA expression of IL-6 in piglets after LPS challenge (0.01 < p < 0.05). β-glucan supplementation enriched the short-chain fatty acid-producing bacteria, such as Agathobacter and Subdoligranulum (0.01 < p < 0.05), and increased the concentrations of propionate and butyrate (0.01 < p < 0.05). In conclusion, pre-feeding β-glucan can enhance piglet immunity and promote piglet growth by influencing gut microbiota composition and metabolism, and alleviate intestinal damage after LPS challenge. |
| format | Article |
| id | doaj-art-ea4f903d6ff24da49d421aff410ec5a2 |
| institution | DOAJ |
| issn | 2297-1769 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Veterinary Science |
| spelling | doaj-art-ea4f903d6ff24da49d421aff410ec5a22025-08-20T02:47:04ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-03-011210.3389/fvets.2025.15338721533872The effect of a water-soluble β-glucan on intestinal immunity and microbiota in LPS-challenged pigletsYuliang Wu0Yuliang Wu1Yuxin Li2Yuxin Li3Mengli Chen4Mengli Chen5Juan Zhao6Xia Xiong7Xia Xiong8Xia Xiong9Chen Guang Olnood10Yundi Gao11Fei Wang12Can Peng13Can Peng14Miao Liu15Miao Liu16Chunxia Huang17Jianzhong Li18Liuqin He19Huansheng Yang20Yulong Yin21Yulong Yin22Yulong Yin23Laboratory of Animal Nutrition and Human Health, College of Life Sciences, Hunan Normal University, Changsha, ChinaCAS Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Hunan Province Key Laboratory of Animal Nutritional Physiology and Metabolic Process, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, ChinaLaboratory of Animal Nutrition and Human Health, College of Life Sciences, Hunan Normal University, Changsha, ChinaCAS Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Hunan Province Key Laboratory of Animal Nutritional Physiology and Metabolic Process, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, ChinaLaboratory of Animal Nutrition and Human Health, College of Life Sciences, Hunan Normal University, Changsha, ChinaCAS Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Hunan Province Key Laboratory of Animal Nutritional Physiology and Metabolic Process, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, ChinaSichuan Synlight Biotech Ltd., Chengdu, ChinaCAS Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Hunan Province Key Laboratory of Animal Nutritional Physiology and Metabolic Process, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaChangsha Medical University, Changsha, ChinaSichuan Synlight Biotech Ltd., Chengdu, ChinaSichuan Synlight Biotech Ltd., Chengdu, ChinaSichuan Synlight Biotech Ltd., Chengdu, ChinaCAS Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Hunan Province Key Laboratory of Animal Nutritional Physiology and Metabolic Process, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, ChinaJilin Da’an Agro-Ecosystem National Observation Research Station, Changchun, ChinaChangsha Medical University, Changsha, ChinaLaboratory of Animal Nutrition and Human Health, College of Life Sciences, Hunan Normal University, Changsha, ChinaLaboratory of Animal Nutrition and Human Health, College of Life Sciences, Hunan Normal University, Changsha, ChinaLaboratory of Animal Nutrition and Human Health, College of Life Sciences, Hunan Normal University, Changsha, ChinaLaboratory of Animal Nutrition and Human Health, College of Life Sciences, Hunan Normal University, Changsha, ChinaCAS Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Hunan Province Key Laboratory of Animal Nutritional Physiology and Metabolic Process, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaThe intestine is the largest immune and barrier organ in the body, and diarrhea and even death during piglet development are related to dysfunction caused by intestinal barrier damage and inflammation. A water-soluble β-glucan produced by Agrobacterium ZX09 has been shown to have a beneficial effect on gastrointestinal health. The main objective of this study was to investigate whether pre-feeding β-glucan has a protective effect on LPS-induced immune stress in piglets. In this study, 24 weaned piglets (21-day-old; 6.64 ± 0.16 kg) were assigned to 4 treatments in a two × two factorial design with diet (with or without β-glucan) and immunological challenge (saline or LPS). Piglets were challenged with saline or LPS after 39 days of feeding 0 or 200 mg/kg β-glucan. The results demonstrated that β-glucan supplementation increased the average daily weight gain and daily feed intake, and decreased diarrhea rate of piglets. Intestinal inflammation symptoms and histological changes in LPS-challenged piglets were alleviated by pre-feeding of β-glucan. β-glucan supplementation reduced serum IL-1β (interleukin-1β) and NO (nitric oxide) secretion in piglets after LPS challenge (0.01 < p < 0.05). Supplementation with β-glucan downregulated the mRNA expression of IL-6 in piglets after LPS challenge (0.01 < p < 0.05). β-glucan supplementation enriched the short-chain fatty acid-producing bacteria, such as Agathobacter and Subdoligranulum (0.01 < p < 0.05), and increased the concentrations of propionate and butyrate (0.01 < p < 0.05). In conclusion, pre-feeding β-glucan can enhance piglet immunity and promote piglet growth by influencing gut microbiota composition and metabolism, and alleviate intestinal damage after LPS challenge.https://www.frontiersin.org/articles/10.3389/fvets.2025.1533872/fullβ-glucanpigletdiarrheaintestinal inflammationlipopolysaccharideintestinal microbiota |
| spellingShingle | Yuliang Wu Yuliang Wu Yuxin Li Yuxin Li Mengli Chen Mengli Chen Juan Zhao Xia Xiong Xia Xiong Xia Xiong Chen Guang Olnood Yundi Gao Fei Wang Can Peng Can Peng Miao Liu Miao Liu Chunxia Huang Jianzhong Li Liuqin He Huansheng Yang Yulong Yin Yulong Yin Yulong Yin The effect of a water-soluble β-glucan on intestinal immunity and microbiota in LPS-challenged piglets Frontiers in Veterinary Science β-glucan piglet diarrhea intestinal inflammation lipopolysaccharide intestinal microbiota |
| title | The effect of a water-soluble β-glucan on intestinal immunity and microbiota in LPS-challenged piglets |
| title_full | The effect of a water-soluble β-glucan on intestinal immunity and microbiota in LPS-challenged piglets |
| title_fullStr | The effect of a water-soluble β-glucan on intestinal immunity and microbiota in LPS-challenged piglets |
| title_full_unstemmed | The effect of a water-soluble β-glucan on intestinal immunity and microbiota in LPS-challenged piglets |
| title_short | The effect of a water-soluble β-glucan on intestinal immunity and microbiota in LPS-challenged piglets |
| title_sort | effect of a water soluble β glucan on intestinal immunity and microbiota in lps challenged piglets |
| topic | β-glucan piglet diarrhea intestinal inflammation lipopolysaccharide intestinal microbiota |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2025.1533872/full |
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