The Expression of Netrin-1 in the Thymus and Its Effects on Thymocyte Adhesion and Migration

Netrin-1, a known axon guidance molecule, being a secreted laminin-related molecule, has been suggested to be involved in multiple physiological and pathological conditions, such as organogenesis, angiogenesis, tumorigenesis, and inflammation-mediated tissue injury. However, its function in thymocyt...

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Main Authors: Xiao-Kai Guo, Yuan-Feng Liu, Yu Zhou, Xiu-Yuan Sun, Xiao-Ping Qian, Yu Zhang, Jun Zhang
Format: Article
Language:English
Published: Wiley 2013-01-01
Series:Clinical and Developmental Immunology
Online Access:http://dx.doi.org/10.1155/2013/462152
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author Xiao-Kai Guo
Yuan-Feng Liu
Yu Zhou
Xiu-Yuan Sun
Xiao-Ping Qian
Yu Zhang
Jun Zhang
author_facet Xiao-Kai Guo
Yuan-Feng Liu
Yu Zhou
Xiu-Yuan Sun
Xiao-Ping Qian
Yu Zhang
Jun Zhang
author_sort Xiao-Kai Guo
collection DOAJ
description Netrin-1, a known axon guidance molecule, being a secreted laminin-related molecule, has been suggested to be involved in multiple physiological and pathological conditions, such as organogenesis, angiogenesis, tumorigenesis, and inflammation-mediated tissue injury. However, its function in thymocyte development is still unknown. Here, we demonstrate that Netrin-1 is expressed in mouse thymus tissue and is primarily expressed in thymic stromal cells, and the expression of Netrin-1 in thymocytes can be induced by anti-CD3 antibody or IL-7 treatment. Importantly, Netrin-1 mediates the adhesion of thymocytes, and this effect is comparable to or greater than that of fibronectin. Furthermore, Netrin-1 specifically promotes the chemotaxis of CXCL12. These suggest that Netrin-1 may play an important role in thymocyte development.
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publishDate 2013-01-01
publisher Wiley
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series Clinical and Developmental Immunology
spelling doaj-art-ea4f3f40b8264a8ebb1959e6cff5e31b2025-08-20T03:26:34ZengWileyClinical and Developmental Immunology1740-25221740-25302013-01-01201310.1155/2013/462152462152The Expression of Netrin-1 in the Thymus and Its Effects on Thymocyte Adhesion and MigrationXiao-Kai Guo0Yuan-Feng Liu1Yu Zhou2Xiu-Yuan Sun3Xiao-Ping Qian4Yu Zhang5Jun Zhang6Department of Immunology, Key Laboratory of Medical Immunology (Ministry of Health), School of Basic Medical Sciences, Peking University Health Science Center, No. 38 Xueyuan Road, Beijing 100191, ChinaDepartment of Immunology, Key Laboratory of Medical Immunology (Ministry of Health), School of Basic Medical Sciences, Peking University Health Science Center, No. 38 Xueyuan Road, Beijing 100191, ChinaDepartment of Immunology, Key Laboratory of Medical Immunology (Ministry of Health), School of Basic Medical Sciences, Peking University Health Science Center, No. 38 Xueyuan Road, Beijing 100191, ChinaDepartment of Immunology, Key Laboratory of Medical Immunology (Ministry of Health), School of Basic Medical Sciences, Peking University Health Science Center, No. 38 Xueyuan Road, Beijing 100191, ChinaDepartment of Immunology, Key Laboratory of Medical Immunology (Ministry of Health), School of Basic Medical Sciences, Peking University Health Science Center, No. 38 Xueyuan Road, Beijing 100191, ChinaDepartment of Immunology, Key Laboratory of Medical Immunology (Ministry of Health), School of Basic Medical Sciences, Peking University Health Science Center, No. 38 Xueyuan Road, Beijing 100191, ChinaDepartment of Immunology, Key Laboratory of Medical Immunology (Ministry of Health), School of Basic Medical Sciences, Peking University Health Science Center, No. 38 Xueyuan Road, Beijing 100191, ChinaNetrin-1, a known axon guidance molecule, being a secreted laminin-related molecule, has been suggested to be involved in multiple physiological and pathological conditions, such as organogenesis, angiogenesis, tumorigenesis, and inflammation-mediated tissue injury. However, its function in thymocyte development is still unknown. Here, we demonstrate that Netrin-1 is expressed in mouse thymus tissue and is primarily expressed in thymic stromal cells, and the expression of Netrin-1 in thymocytes can be induced by anti-CD3 antibody or IL-7 treatment. Importantly, Netrin-1 mediates the adhesion of thymocytes, and this effect is comparable to or greater than that of fibronectin. Furthermore, Netrin-1 specifically promotes the chemotaxis of CXCL12. These suggest that Netrin-1 may play an important role in thymocyte development.http://dx.doi.org/10.1155/2013/462152
spellingShingle Xiao-Kai Guo
Yuan-Feng Liu
Yu Zhou
Xiu-Yuan Sun
Xiao-Ping Qian
Yu Zhang
Jun Zhang
The Expression of Netrin-1 in the Thymus and Its Effects on Thymocyte Adhesion and Migration
Clinical and Developmental Immunology
title The Expression of Netrin-1 in the Thymus and Its Effects on Thymocyte Adhesion and Migration
title_full The Expression of Netrin-1 in the Thymus and Its Effects on Thymocyte Adhesion and Migration
title_fullStr The Expression of Netrin-1 in the Thymus and Its Effects on Thymocyte Adhesion and Migration
title_full_unstemmed The Expression of Netrin-1 in the Thymus and Its Effects on Thymocyte Adhesion and Migration
title_short The Expression of Netrin-1 in the Thymus and Its Effects on Thymocyte Adhesion and Migration
title_sort expression of netrin 1 in the thymus and its effects on thymocyte adhesion and migration
url http://dx.doi.org/10.1155/2013/462152
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