Fast detection of protein kinase B in chrysin treated colorectal cancer cells using a novel multicore microfiber biosensor
Abstract Rapid and accurate determination of target proteins in cells provide essential diagnostic information for early detection of diseases, evaluation of drug responses, and the study of pathophysiological mechanisms. Traditional Western blotting method has been used for the determination, but i...
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| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-12-01
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| Series: | Communications Engineering |
| Online Access: | https://doi.org/10.1038/s44172-024-00332-y |
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| _version_ | 1850086586114375680 |
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| author | Zhen Tian Hongzhuan Xuan Yicun Yao Shengyu Hao Zhichao Zhang Bingyuan Zhang Jingao Zhang Liqiang Zhang Xinzhu Sang Jinhui Yuan Gerald Farrell Qiang Wu |
| author_facet | Zhen Tian Hongzhuan Xuan Yicun Yao Shengyu Hao Zhichao Zhang Bingyuan Zhang Jingao Zhang Liqiang Zhang Xinzhu Sang Jinhui Yuan Gerald Farrell Qiang Wu |
| author_sort | Zhen Tian |
| collection | DOAJ |
| description | Abstract Rapid and accurate determination of target proteins in cells provide essential diagnostic information for early detection of diseases, evaluation of drug responses, and the study of pathophysiological mechanisms. Traditional Western blotting method has been used for the determination, but it is complex, time-consuming, and semi-quantitative. Here, a tapered seven-core fiber (TSCF) biosensor was designed and fabricated. By immobilizing protein kinase B (PKB), also known as AKT, antibody onto TSCF surface, the microfiber biosensor can be used for quantitatively detecting the AKT level in solution concentrations as low as 0.26 ng/mL. To test the reliability of the TSCF sensing method in a medical application, the TSCF biosensor was used to study the relationship between chrysin’s anticancer effect and the concentration of AKT in a human colorectal cancer cell line (LoVo cells). The results reveal that the inhibitory effect of chrysin on LoVo cells is positively correlated with the dose, agreeing well with the equivalent results using the traditional Western blotting method. |
| format | Article |
| id | doaj-art-ea4b49fc05424b258ae2cdd124541fa6 |
| institution | DOAJ |
| issn | 2731-3395 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Engineering |
| spelling | doaj-art-ea4b49fc05424b258ae2cdd124541fa62025-08-20T02:43:25ZengNature PortfolioCommunications Engineering2731-33952024-12-01311810.1038/s44172-024-00332-yFast detection of protein kinase B in chrysin treated colorectal cancer cells using a novel multicore microfiber biosensorZhen Tian0Hongzhuan Xuan1Yicun Yao2Shengyu Hao3Zhichao Zhang4Bingyuan Zhang5Jingao Zhang6Liqiang Zhang7Xinzhu Sang8Jinhui Yuan9Gerald Farrell10Qiang Wu11State Key Laboratory of Information Photonics and Optical Communications, Beijing University of Posts and TelecommunicationsSchool of Life Science, Liaocheng UniversitySchool of Physics Sciences and Information Technology, Liaocheng UniversitySchool of Physics Sciences and Information Technology, Liaocheng UniversityState Key Laboratory of Information Photonics and Optical Communications, Beijing University of Posts and TelecommunicationsSchool of Physics Sciences and Information Technology, Liaocheng UniversityState Key Laboratory of Information Photonics and Optical Communications, Beijing University of Posts and TelecommunicationsSchool of Physics Sciences and Information Technology, Liaocheng UniversityState Key Laboratory of Information Photonics and Optical Communications, Beijing University of Posts and TelecommunicationsState Key Laboratory of Information Photonics and Optical Communications, Beijing University of Posts and TelecommunicationsPhotonics Research Centre, School of Electrical and Electronic Engineering, City Campus, Technological University DublinState Key Laboratory of Information Photonics and Optical Communications, Beijing University of Posts and TelecommunicationsAbstract Rapid and accurate determination of target proteins in cells provide essential diagnostic information for early detection of diseases, evaluation of drug responses, and the study of pathophysiological mechanisms. Traditional Western blotting method has been used for the determination, but it is complex, time-consuming, and semi-quantitative. Here, a tapered seven-core fiber (TSCF) biosensor was designed and fabricated. By immobilizing protein kinase B (PKB), also known as AKT, antibody onto TSCF surface, the microfiber biosensor can be used for quantitatively detecting the AKT level in solution concentrations as low as 0.26 ng/mL. To test the reliability of the TSCF sensing method in a medical application, the TSCF biosensor was used to study the relationship between chrysin’s anticancer effect and the concentration of AKT in a human colorectal cancer cell line (LoVo cells). The results reveal that the inhibitory effect of chrysin on LoVo cells is positively correlated with the dose, agreeing well with the equivalent results using the traditional Western blotting method.https://doi.org/10.1038/s44172-024-00332-y |
| spellingShingle | Zhen Tian Hongzhuan Xuan Yicun Yao Shengyu Hao Zhichao Zhang Bingyuan Zhang Jingao Zhang Liqiang Zhang Xinzhu Sang Jinhui Yuan Gerald Farrell Qiang Wu Fast detection of protein kinase B in chrysin treated colorectal cancer cells using a novel multicore microfiber biosensor Communications Engineering |
| title | Fast detection of protein kinase B in chrysin treated colorectal cancer cells using a novel multicore microfiber biosensor |
| title_full | Fast detection of protein kinase B in chrysin treated colorectal cancer cells using a novel multicore microfiber biosensor |
| title_fullStr | Fast detection of protein kinase B in chrysin treated colorectal cancer cells using a novel multicore microfiber biosensor |
| title_full_unstemmed | Fast detection of protein kinase B in chrysin treated colorectal cancer cells using a novel multicore microfiber biosensor |
| title_short | Fast detection of protein kinase B in chrysin treated colorectal cancer cells using a novel multicore microfiber biosensor |
| title_sort | fast detection of protein kinase b in chrysin treated colorectal cancer cells using a novel multicore microfiber biosensor |
| url | https://doi.org/10.1038/s44172-024-00332-y |
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