Effect of Raspberry (<i>Rubus indeaus</i> L.) Juice Fermented by <i>Limosilactobacillus fermentum</i> FUA033 on the Human Gut Microbiota Cultured In Vitro: A Multi-Omics Approach
The gut microbiota plays important functions in human health and influences immune responses, metabolic processes, and several physiological activities. The modulation of the gut microbiota through dietary interventions has emerged as a promising approach, leading to significant interest in the deve...
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MDPI AG
2025-05-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/14/10/1796 |
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| author | Ziyan Hua Yunfan Lv Han Zhang Tianyi Mao Ruyu Xv Mingxuan Pan Yadong Hu Shu Liu Yaowei Fang |
| author_facet | Ziyan Hua Yunfan Lv Han Zhang Tianyi Mao Ruyu Xv Mingxuan Pan Yadong Hu Shu Liu Yaowei Fang |
| author_sort | Ziyan Hua |
| collection | DOAJ |
| description | The gut microbiota plays important functions in human health and influences immune responses, metabolic processes, and several physiological activities. The modulation of the gut microbiota through dietary interventions has emerged as a promising approach, leading to significant interest in the development of functional foods that provide health benefits. In this context, our study investigated the effects of raspberry juice fermented by <i>Limosilactobacillus fermentum</i> FUA033 on the structure and metabolism of the gut microbiota. We performed 16S rRNA gene sequencing and nontargeted metabolomics analyses to evaluate changes in the microbial composition and metabolite profiles resulting from fermentation. Our findings revealed that fermented raspberry juice considerably increased the gut microbial diversity and promoted the abundance of beneficial genera. Fermentation substantially increased the production of short-chain fatty acids, such as acetate and butyrate, which increased from 30.09 ± 5.23 mmol/L to 43.07 ± 3.31 mmol/L, and from 7.72 ± 1.72 mmol/L to 15.01 ± 1.26 mmol/L, respectively. Metabolomic analyses also showed significant enhancements in amino acid metabolism pathways, particularly those involving tyrosine, arginine, and proline. These results highlight the potential of fermented raspberry juice as a functional food to improve gut health and metabolic functions. |
| format | Article |
| id | doaj-art-ea4a3f9f6f414846a5fcb31f0810dadb |
| institution | DOAJ |
| issn | 2304-8158 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj-art-ea4a3f9f6f414846a5fcb31f0810dadb2025-08-20T03:14:31ZengMDPI AGFoods2304-81582025-05-011410179610.3390/foods14101796Effect of Raspberry (<i>Rubus indeaus</i> L.) Juice Fermented by <i>Limosilactobacillus fermentum</i> FUA033 on the Human Gut Microbiota Cultured In Vitro: A Multi-Omics ApproachZiyan Hua0Yunfan Lv1Han Zhang2Tianyi Mao3Ruyu Xv4Mingxuan Pan5Yadong Hu6Shu Liu7Yaowei Fang8Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang 222005, ChinaKey Laboratory of Human Functional Genomics of Jiangsu Province, Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing 210029, ChinaCo-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang 222005, ChinaCo-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang 222005, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment, School of Food Science and Engineering, Jiangsu Ocean University, Lianyungang 222005, ChinaJiangsu Innovation Center of Marine Bioresources, Jiangsu Coast Development Investment Co., Ltd., Nanjing 210019, ChinaJiangsu Innovation Center of Marine Bioresources, Jiangsu Coast Development Investment Co., Ltd., Nanjing 210019, ChinaCo-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang 222005, ChinaCo-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang 222005, ChinaThe gut microbiota plays important functions in human health and influences immune responses, metabolic processes, and several physiological activities. The modulation of the gut microbiota through dietary interventions has emerged as a promising approach, leading to significant interest in the development of functional foods that provide health benefits. In this context, our study investigated the effects of raspberry juice fermented by <i>Limosilactobacillus fermentum</i> FUA033 on the structure and metabolism of the gut microbiota. We performed 16S rRNA gene sequencing and nontargeted metabolomics analyses to evaluate changes in the microbial composition and metabolite profiles resulting from fermentation. Our findings revealed that fermented raspberry juice considerably increased the gut microbial diversity and promoted the abundance of beneficial genera. Fermentation substantially increased the production of short-chain fatty acids, such as acetate and butyrate, which increased from 30.09 ± 5.23 mmol/L to 43.07 ± 3.31 mmol/L, and from 7.72 ± 1.72 mmol/L to 15.01 ± 1.26 mmol/L, respectively. Metabolomic analyses also showed significant enhancements in amino acid metabolism pathways, particularly those involving tyrosine, arginine, and proline. These results highlight the potential of fermented raspberry juice as a functional food to improve gut health and metabolic functions.https://www.mdpi.com/2304-8158/14/10/1796metabolomicsgut microbiota<i>Limosilactobacillus fermentum</i> FUA033fermentationshort-chain fatty acids |
| spellingShingle | Ziyan Hua Yunfan Lv Han Zhang Tianyi Mao Ruyu Xv Mingxuan Pan Yadong Hu Shu Liu Yaowei Fang Effect of Raspberry (<i>Rubus indeaus</i> L.) Juice Fermented by <i>Limosilactobacillus fermentum</i> FUA033 on the Human Gut Microbiota Cultured In Vitro: A Multi-Omics Approach Foods metabolomics gut microbiota <i>Limosilactobacillus fermentum</i> FUA033 fermentation short-chain fatty acids |
| title | Effect of Raspberry (<i>Rubus indeaus</i> L.) Juice Fermented by <i>Limosilactobacillus fermentum</i> FUA033 on the Human Gut Microbiota Cultured In Vitro: A Multi-Omics Approach |
| title_full | Effect of Raspberry (<i>Rubus indeaus</i> L.) Juice Fermented by <i>Limosilactobacillus fermentum</i> FUA033 on the Human Gut Microbiota Cultured In Vitro: A Multi-Omics Approach |
| title_fullStr | Effect of Raspberry (<i>Rubus indeaus</i> L.) Juice Fermented by <i>Limosilactobacillus fermentum</i> FUA033 on the Human Gut Microbiota Cultured In Vitro: A Multi-Omics Approach |
| title_full_unstemmed | Effect of Raspberry (<i>Rubus indeaus</i> L.) Juice Fermented by <i>Limosilactobacillus fermentum</i> FUA033 on the Human Gut Microbiota Cultured In Vitro: A Multi-Omics Approach |
| title_short | Effect of Raspberry (<i>Rubus indeaus</i> L.) Juice Fermented by <i>Limosilactobacillus fermentum</i> FUA033 on the Human Gut Microbiota Cultured In Vitro: A Multi-Omics Approach |
| title_sort | effect of raspberry i rubus indeaus i l juice fermented by i limosilactobacillus fermentum i fua033 on the human gut microbiota cultured in vitro a multi omics approach |
| topic | metabolomics gut microbiota <i>Limosilactobacillus fermentum</i> FUA033 fermentation short-chain fatty acids |
| url | https://www.mdpi.com/2304-8158/14/10/1796 |
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