Toxicity of fusaric acid and beauvericin in tissue-cultured banana 'Grand Nain' and 'Lakatan'
Fusarium oxysporum forma specialis cubense (Foc) produces toxins known to contribute to virulence and infection in the host. Fusaric acid (FA) and beauvericin (BEA) are major toxins contributing to Foc virulence in the host plant. Recent advancements allow the production of disease-resistant crops v...
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Maximum Academic Press
2023-01-01
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| Series: | Technology in Horticulture |
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| Online Access: | https://www.maxapress.com/article/doi/10.48130/TIH-2023-0015 |
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| author | Maria Angela Cruz Carolyn Alcasid Cyrose Silvosa Millado Mark Angelo Balendres |
| author_facet | Maria Angela Cruz Carolyn Alcasid Cyrose Silvosa Millado Mark Angelo Balendres |
| author_sort | Maria Angela Cruz |
| collection | DOAJ |
| description | Fusarium oxysporum forma specialis cubense (Foc) produces toxins known to contribute to virulence and infection in the host. Fusaric acid (FA) and beauvericin (BEA) are major toxins contributing to Foc virulence in the host plant. Recent advancements allow the production of disease-resistant crops via cell selection, a process that involves resistance screening of somaclones using phytotoxin exposure. Determination of the appropriate concentration is an important step for the toxin-based selection of disease-resistant plants. In this study, the toxicity of FA (0, 5, 10, 20, 30, 50, and 100 μm) and BEA (0, 5, 10, and 20 μm) in different tissues of banana cv. ‘Grand Nain’ and 'Lakatan' were investigated. Overall results indicated a positive relationship between the toxin concentration and plant sensitivity, as indicated by the degree of vascular browning rate. Results demonstrated that lower concentrations of BEA are required for phytotoxicity than in FA. Furthermore, a higher degree of vascular browning was recorded in the test tissues of ‘Lakatan’ treated with FA and BEA than in the ‘Grand Nain’ genotype suggesting genotype-dependent sensitivity of banana to phytotoxins. To our knowledge, this study is the first to investigate the phytotoxicity of FA and BEA in callus cultures of banana ‘Lakatan.’ |
| format | Article |
| id | doaj-art-ea44049be8fe44e7be9924e810dfe577 |
| institution | DOAJ |
| issn | 2833-4337 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Maximum Academic Press |
| record_format | Article |
| series | Technology in Horticulture |
| spelling | doaj-art-ea44049be8fe44e7be9924e810dfe5772025-08-20T03:18:42ZengMaximum Academic PressTechnology in Horticulture2833-43372023-01-01311610.48130/TIH-2023-0015TIH-2023-0015Toxicity of fusaric acid and beauvericin in tissue-cultured banana 'Grand Nain' and 'Lakatan'Maria Angela Cruz0Carolyn Alcasid1Cyrose Silvosa Millado2Mark Angelo Balendres3Institute of Plant Breeding, College of Agriculture and Food Science, University of the Philippines, Los Baños, Laguna, Philippines 4031Institute of Plant Breeding, College of Agriculture and Food Science, University of the Philippines, Los Baños, Laguna, Philippines 4031Department of Biological Sciences and Environmental Studies, College of Science and Mathematics, University of the Philippines Mindanao, Philippines 8022Institute of Plant Breeding, College of Agriculture and Food Science, University of the Philippines, Los Baños, Laguna, Philippines 4031Fusarium oxysporum forma specialis cubense (Foc) produces toxins known to contribute to virulence and infection in the host. Fusaric acid (FA) and beauvericin (BEA) are major toxins contributing to Foc virulence in the host plant. Recent advancements allow the production of disease-resistant crops via cell selection, a process that involves resistance screening of somaclones using phytotoxin exposure. Determination of the appropriate concentration is an important step for the toxin-based selection of disease-resistant plants. In this study, the toxicity of FA (0, 5, 10, 20, 30, 50, and 100 μm) and BEA (0, 5, 10, and 20 μm) in different tissues of banana cv. ‘Grand Nain’ and 'Lakatan' were investigated. Overall results indicated a positive relationship between the toxin concentration and plant sensitivity, as indicated by the degree of vascular browning rate. Results demonstrated that lower concentrations of BEA are required for phytotoxicity than in FA. Furthermore, a higher degree of vascular browning was recorded in the test tissues of ‘Lakatan’ treated with FA and BEA than in the ‘Grand Nain’ genotype suggesting genotype-dependent sensitivity of banana to phytotoxins. To our knowledge, this study is the first to investigate the phytotoxicity of FA and BEA in callus cultures of banana ‘Lakatan.’https://www.maxapress.com/article/doi/10.48130/TIH-2023-0015phytotoxinsmusa spbananafusarium oxysporumfusarium wilt |
| spellingShingle | Maria Angela Cruz Carolyn Alcasid Cyrose Silvosa Millado Mark Angelo Balendres Toxicity of fusaric acid and beauvericin in tissue-cultured banana 'Grand Nain' and 'Lakatan' Technology in Horticulture phytotoxins musa sp banana fusarium oxysporum fusarium wilt |
| title | Toxicity of fusaric acid and beauvericin in tissue-cultured banana 'Grand Nain' and 'Lakatan' |
| title_full | Toxicity of fusaric acid and beauvericin in tissue-cultured banana 'Grand Nain' and 'Lakatan' |
| title_fullStr | Toxicity of fusaric acid and beauvericin in tissue-cultured banana 'Grand Nain' and 'Lakatan' |
| title_full_unstemmed | Toxicity of fusaric acid and beauvericin in tissue-cultured banana 'Grand Nain' and 'Lakatan' |
| title_short | Toxicity of fusaric acid and beauvericin in tissue-cultured banana 'Grand Nain' and 'Lakatan' |
| title_sort | toxicity of fusaric acid and beauvericin in tissue cultured banana grand nain and lakatan |
| topic | phytotoxins musa sp banana fusarium oxysporum fusarium wilt |
| url | https://www.maxapress.com/article/doi/10.48130/TIH-2023-0015 |
| work_keys_str_mv | AT mariaangelacruz toxicityoffusaricacidandbeauvericinintissueculturedbananagrandnainandlakatan AT carolynalcasid toxicityoffusaricacidandbeauvericinintissueculturedbananagrandnainandlakatan AT cyrosesilvosamillado toxicityoffusaricacidandbeauvericinintissueculturedbananagrandnainandlakatan AT markangelobalendres toxicityoffusaricacidandbeauvericinintissueculturedbananagrandnainandlakatan |