LYMPHOCYTE SUBPOPULATIONS IN GASTRIC AND COLORECTAL CANCER PATIENTS AFTER IMMUNOTHERAPY WITH ACTIVATED LYMPHOCYTES
Phenotyping of peripheral blood lymphocytes and immune activation markers of activation (HLADR, CD38, CD69, CD314, CD25) was performed in thirteen patients with disseminated forms of stomach and rectal cancer before cell treatment, and following adoptive immunotherapy with activated lymphocytes. It...
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| Format: | Article |
| Language: | Russian |
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St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2014-11-01
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| Series: | Медицинская иммунология |
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| Online Access: | https://www.mimmun.ru/mimmun/article/view/732 |
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| author | E. V. Abakushina Yu. V. Marizina G. S. Neprina I. A. Pasova B. A. Berdov |
| author_facet | E. V. Abakushina Yu. V. Marizina G. S. Neprina I. A. Pasova B. A. Berdov |
| author_sort | E. V. Abakushina |
| collection | DOAJ |
| description | Phenotyping of peripheral blood lymphocytes and immune activation markers of activation (HLADR, CD38, CD69, CD314, CD25) was performed in thirteen patients with disseminated forms of stomach and rectal cancer before cell treatment, and following adoptive immunotherapy with activated lymphocytes. It wasshown that the lymphocytes are well activated and are able to proliferate in vitro. It was revealed that the relative content of Tregs and NK cells is increased in these patients. After the courses of immunotherapy, initially low levels of B-cells (average 5%) remained in all the patients, whereas concentration of Тregs didn’t change. Increased expression of activation markers was revealed for all lymphocyte subsets (CD25, CD314, CD38, HLA-DR) and, especially, for T-lymphocytes (CD3+HLA-DR, CD3+CD38+). Significant decrease of T helpers, activated NK-cells (CD16+CD314+) and CD4+/CD8+ was noted in peripheral blood. A non-significant elevation in mature lymphocytes (CD45RO+) and reduced content of young lymphocytes (CD45RA+) were revealed. Adoptive immunotherapy is safe and well tolerated, being characterized by lack of side effects, and it may be recommended as a complement to conventional radio- and chemotherapy. |
| format | Article |
| id | doaj-art-ea42dd37277a49c1904ef89e931365df |
| institution | Kabale University |
| issn | 1563-0625 2313-741X |
| language | Russian |
| publishDate | 2014-11-01 |
| publisher | St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
| record_format | Article |
| series | Медицинская иммунология |
| spelling | doaj-art-ea42dd37277a49c1904ef89e931365df2025-08-20T04:00:09ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2014-11-0116544945610.15789/1563-0625-2014-5-449-456712LYMPHOCYTE SUBPOPULATIONS IN GASTRIC AND COLORECTAL CANCER PATIENTS AFTER IMMUNOTHERAPY WITH ACTIVATED LYMPHOCYTESE. V. Abakushina0Yu. V. Marizina1G. S. Neprina2I. A. Pasova3B. A. Berdov4Medical Radiological Research Center, Russian Ministry of Health Care, Obninsk, Russian FederationMedical Radiological Research Center, Russian Ministry of Health Care, Obninsk, Russian FederationMedical Radiological Research Center, Russian Ministry of Health Care, Obninsk, Russian FederationMedical Radiological Research Center, Russian Ministry of Health Care, Obninsk, Russian FederationMedical Radiological Research Center, Russian Ministry of Health Care, Obninsk, Russian FederationPhenotyping of peripheral blood lymphocytes and immune activation markers of activation (HLADR, CD38, CD69, CD314, CD25) was performed in thirteen patients with disseminated forms of stomach and rectal cancer before cell treatment, and following adoptive immunotherapy with activated lymphocytes. It wasshown that the lymphocytes are well activated and are able to proliferate in vitro. It was revealed that the relative content of Tregs and NK cells is increased in these patients. After the courses of immunotherapy, initially low levels of B-cells (average 5%) remained in all the patients, whereas concentration of Тregs didn’t change. Increased expression of activation markers was revealed for all lymphocyte subsets (CD25, CD314, CD38, HLA-DR) and, especially, for T-lymphocytes (CD3+HLA-DR, CD3+CD38+). Significant decrease of T helpers, activated NK-cells (CD16+CD314+) and CD4+/CD8+ was noted in peripheral blood. A non-significant elevation in mature lymphocytes (CD45RO+) and reduced content of young lymphocytes (CD45RA+) were revealed. Adoptive immunotherapy is safe and well tolerated, being characterized by lack of side effects, and it may be recommended as a complement to conventional radio- and chemotherapy.https://www.mimmun.ru/mimmun/article/view/732adoptive immunotherapyactivated lymphocytesgastric and colorectal cancerlymphocyte phenotypeperipheral blood |
| spellingShingle | E. V. Abakushina Yu. V. Marizina G. S. Neprina I. A. Pasova B. A. Berdov LYMPHOCYTE SUBPOPULATIONS IN GASTRIC AND COLORECTAL CANCER PATIENTS AFTER IMMUNOTHERAPY WITH ACTIVATED LYMPHOCYTES Медицинская иммунология adoptive immunotherapy activated lymphocytes gastric and colorectal cancer lymphocyte phenotype peripheral blood |
| title | LYMPHOCYTE SUBPOPULATIONS IN GASTRIC AND COLORECTAL CANCER PATIENTS AFTER IMMUNOTHERAPY WITH ACTIVATED LYMPHOCYTES |
| title_full | LYMPHOCYTE SUBPOPULATIONS IN GASTRIC AND COLORECTAL CANCER PATIENTS AFTER IMMUNOTHERAPY WITH ACTIVATED LYMPHOCYTES |
| title_fullStr | LYMPHOCYTE SUBPOPULATIONS IN GASTRIC AND COLORECTAL CANCER PATIENTS AFTER IMMUNOTHERAPY WITH ACTIVATED LYMPHOCYTES |
| title_full_unstemmed | LYMPHOCYTE SUBPOPULATIONS IN GASTRIC AND COLORECTAL CANCER PATIENTS AFTER IMMUNOTHERAPY WITH ACTIVATED LYMPHOCYTES |
| title_short | LYMPHOCYTE SUBPOPULATIONS IN GASTRIC AND COLORECTAL CANCER PATIENTS AFTER IMMUNOTHERAPY WITH ACTIVATED LYMPHOCYTES |
| title_sort | lymphocyte subpopulations in gastric and colorectal cancer patients after immunotherapy with activated lymphocytes |
| topic | adoptive immunotherapy activated lymphocytes gastric and colorectal cancer lymphocyte phenotype peripheral blood |
| url | https://www.mimmun.ru/mimmun/article/view/732 |
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