A Morphometric Analysis of Nucleoli in Cultured Carcinoma Cells of the Human Thyroid
Cells from 7 patients operated on for thyroid cancer were investigated. Samples of cells from the carcinoma and from the normal thyroid tissue were cultured with and without TSH stimulation. For light microscopy, serial sections of cells were cut and the size of nucleoli was measured and the number...
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| Format: | Article |
| Language: | English |
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Wiley
1998-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/1998/825184 |
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| author | Lars Andersen Lars Kayser Niels Keiding Jens Thomsen |
| author_facet | Lars Andersen Lars Kayser Niels Keiding Jens Thomsen |
| author_sort | Lars Andersen |
| collection | DOAJ |
| description | Cells from 7 patients operated on for thyroid cancer were investigated. Samples of cells from the carcinoma and from the normal thyroid tissue were cultured with and without TSH stimulation. For light microscopy, serial sections of cells were cut and the size of nucleoli was measured and the number of nucleoli per cell counted. At the electron microscopic level the number and the volume of the fibrillar centres (FC) were estimated taking the Swiss cheese effect into account. The areal densities of FC, the fibrillar and granular component in nucleoli were determined by point counting. The results indicate that the malignant transformation has no influence on the size of the FC, but the observed numbers as well as the total area of FC are larger in cancer cells than in the normal thyroid epithelial cells. The nucleolar density of the fibrillar component is larger and that of the granular component is smaller in thyroid carcinoma cells than in non‐malignant thyroid epithelial cells (p = 0.0001). Thus simple morphometry at the electron microscopic level might be helpful to discriminate between thyroid epithelial cells and thyroid carcinoma cells in culture. |
| format | Article |
| id | doaj-art-ea40ef2b86fe47e18da25bd09b17c0df |
| institution | DOAJ |
| issn | 0921-8912 1878-3651 |
| language | English |
| publishDate | 1998-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-ea40ef2b86fe47e18da25bd09b17c0df2025-08-20T03:20:39ZengWileyAnalytical Cellular Pathology0921-89121878-36511998-01-0116313114010.1155/1998/825184A Morphometric Analysis of Nucleoli in Cultured Carcinoma Cells of the Human ThyroidLars Andersen0Lars Kayser1Niels Keiding2Jens Thomsen3Department of Medical Anatomy, University of Copenhagen, Copenhagen, DenmarkDepartment of Medical Anatomy, University of Copenhagen, Copenhagen, DenmarkDepartment of Biostatistics, University of Copenhagen, Copenhagen, DenmarkDepartment of Surgery, Copenhagen County Hospital Gentofte, Copenhagen, DenmarkCells from 7 patients operated on for thyroid cancer were investigated. Samples of cells from the carcinoma and from the normal thyroid tissue were cultured with and without TSH stimulation. For light microscopy, serial sections of cells were cut and the size of nucleoli was measured and the number of nucleoli per cell counted. At the electron microscopic level the number and the volume of the fibrillar centres (FC) were estimated taking the Swiss cheese effect into account. The areal densities of FC, the fibrillar and granular component in nucleoli were determined by point counting. The results indicate that the malignant transformation has no influence on the size of the FC, but the observed numbers as well as the total area of FC are larger in cancer cells than in the normal thyroid epithelial cells. The nucleolar density of the fibrillar component is larger and that of the granular component is smaller in thyroid carcinoma cells than in non‐malignant thyroid epithelial cells (p = 0.0001). Thus simple morphometry at the electron microscopic level might be helpful to discriminate between thyroid epithelial cells and thyroid carcinoma cells in culture.http://dx.doi.org/10.1155/1998/825184 |
| spellingShingle | Lars Andersen Lars Kayser Niels Keiding Jens Thomsen A Morphometric Analysis of Nucleoli in Cultured Carcinoma Cells of the Human Thyroid Analytical Cellular Pathology |
| title | A Morphometric Analysis of Nucleoli in Cultured Carcinoma Cells of the Human Thyroid |
| title_full | A Morphometric Analysis of Nucleoli in Cultured Carcinoma Cells of the Human Thyroid |
| title_fullStr | A Morphometric Analysis of Nucleoli in Cultured Carcinoma Cells of the Human Thyroid |
| title_full_unstemmed | A Morphometric Analysis of Nucleoli in Cultured Carcinoma Cells of the Human Thyroid |
| title_short | A Morphometric Analysis of Nucleoli in Cultured Carcinoma Cells of the Human Thyroid |
| title_sort | morphometric analysis of nucleoli in cultured carcinoma cells of the human thyroid |
| url | http://dx.doi.org/10.1155/1998/825184 |
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