Correction of cellular and humoral links immunity in piglets under the weaning condition
Preventing the development of immunodeficiencies in piglets after weaning is achievable through the parenteral administration of fat-soluble vitamins, macroand microelements, and by analysing their effects on the dynamics of changes in the number and functional activity of T- and B-lymphocyte subpop...
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| Format: | Article |
| Language: | English |
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Zhytomyr National Agroecological University
2025-01-01
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| Series: | Наукові горизонти |
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| Online Access: | https://sciencehorizon.com.ua/en/journals/tom-28-1-2025/korektsiya-klitinnoyi-i-gumoralnoyi-lanok-imunitetu-porosyat-za-umov-vidluchennya |
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| author | Nataliia Ohorodnyk Vitalii Tkachuk Nataliia Motko Andriy Boyko Serhii Pavkovych |
| author_facet | Nataliia Ohorodnyk Vitalii Tkachuk Nataliia Motko Andriy Boyko Serhii Pavkovych |
| author_sort | Nataliia Ohorodnyk |
| collection | DOAJ |
| description | Preventing the development of immunodeficiencies in piglets after weaning is achievable through the parenteral administration of fat-soluble vitamins, macroand microelements, and by analysing their effects on the dynamics of changes in the number and functional activity of T- and B-lymphocyte subpopulations. Accordingly, this study aimed to investigate the effect of a complex liposomal preparation on the cellular and humoral components of the piglet immune system after weaning. A standardised methodology was used to determine the number of different populations and subpopulations of T-lymphocytes in piglet blood samples collected before and at specific intervals after weaning. The analysis of the quantitative composition of T- and B-lymphocytes and the functional activity of T lymphocytes in the blast transformation reaction was conducted using immersion microscopy of smears. The statistical processing of results was carried out through variational nonparametric analysis using biometric methods. The findings revealed that weaning from sows caused a reduction in the number of T-cells of varying degrees of avidity, a decrease in the relative number of T-suppressors in piglets’ blood, and an increase in the number of certain subpopulations of T-helper cells. Conversely, the administration of the liposomal preparation to piglets enhanced the number of various subpopulations of total and active T-lymphocytes and B-lymphocytes in their blood after weaning and increased the activity of lymphoid cells in the blast transformation reaction with phytohaemagglutinin. The observed immunomodulatory effect of the tested drug is attributed to the synergistic combination of fat-soluble vitamins, mineral elements, and arginine in its composition, which effectively prevented the development of stress-induced immunodeficiency in piglets following weaning |
| format | Article |
| id | doaj-art-ea3dad25426c4aea91ab99ad02e4de08 |
| institution | Kabale University |
| issn | 2663-2144 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Zhytomyr National Agroecological University |
| record_format | Article |
| series | Наукові горизонти |
| spelling | doaj-art-ea3dad25426c4aea91ab99ad02e4de082025-02-04T15:04:53ZengZhytomyr National Agroecological UniversityНаукові горизонти2663-21442025-01-0128191810.48077/scihor1.2025.09Correction of cellular and humoral links immunity in piglets under the weaning conditionNataliia Ohorodnyk0https://orcid.org/0000-0002-7428-9973Vitalii Tkachuk1https://orcid.org/0000-0001-6392-4241Nataliia Motko2https://orcid.org/0000-0003-3103-1056Andriy Boyko3https://orcid.org/0000-0002-4629-7108Serhii Pavkovych4https://orcid.org/0000-0002-0844-3071Lviv National Environmental UniversityLviv National Environmental UniversityLviv National University of Veterinary Medicine and Biotechnologies named after S.Z. GzhytskyLviv National University of Veterinary Medicine and Biotechnologies named after S.Z. GzhytskyLviv National Environmental UniversityPreventing the development of immunodeficiencies in piglets after weaning is achievable through the parenteral administration of fat-soluble vitamins, macroand microelements, and by analysing their effects on the dynamics of changes in the number and functional activity of T- and B-lymphocyte subpopulations. Accordingly, this study aimed to investigate the effect of a complex liposomal preparation on the cellular and humoral components of the piglet immune system after weaning. A standardised methodology was used to determine the number of different populations and subpopulations of T-lymphocytes in piglet blood samples collected before and at specific intervals after weaning. The analysis of the quantitative composition of T- and B-lymphocytes and the functional activity of T lymphocytes in the blast transformation reaction was conducted using immersion microscopy of smears. The statistical processing of results was carried out through variational nonparametric analysis using biometric methods. The findings revealed that weaning from sows caused a reduction in the number of T-cells of varying degrees of avidity, a decrease in the relative number of T-suppressors in piglets’ blood, and an increase in the number of certain subpopulations of T-helper cells. Conversely, the administration of the liposomal preparation to piglets enhanced the number of various subpopulations of total and active T-lymphocytes and B-lymphocytes in their blood after weaning and increased the activity of lymphoid cells in the blast transformation reaction with phytohaemagglutinin. The observed immunomodulatory effect of the tested drug is attributed to the synergistic combination of fat-soluble vitamins, mineral elements, and arginine in its composition, which effectively prevented the development of stress-induced immunodeficiency in piglets following weaninghttps://sciencehorizon.com.ua/en/journals/tom-28-1-2025/korektsiya-klitinnoyi-i-gumoralnoyi-lanok-imunitetu-porosyat-za-umov-vidluchennyaimmunodeficienciesliposomal preparationlymphocytes populationsweaningstressarginine |
| spellingShingle | Nataliia Ohorodnyk Vitalii Tkachuk Nataliia Motko Andriy Boyko Serhii Pavkovych Correction of cellular and humoral links immunity in piglets under the weaning condition Наукові горизонти immunodeficiencies liposomal preparation lymphocytes populations weaning stress arginine |
| title | Correction of cellular and humoral links immunity in piglets under the weaning condition |
| title_full | Correction of cellular and humoral links immunity in piglets under the weaning condition |
| title_fullStr | Correction of cellular and humoral links immunity in piglets under the weaning condition |
| title_full_unstemmed | Correction of cellular and humoral links immunity in piglets under the weaning condition |
| title_short | Correction of cellular and humoral links immunity in piglets under the weaning condition |
| title_sort | correction of cellular and humoral links immunity in piglets under the weaning condition |
| topic | immunodeficiencies liposomal preparation lymphocytes populations weaning stress arginine |
| url | https://sciencehorizon.com.ua/en/journals/tom-28-1-2025/korektsiya-klitinnoyi-i-gumoralnoyi-lanok-imunitetu-porosyat-za-umov-vidluchennya |
| work_keys_str_mv | AT nataliiaohorodnyk correctionofcellularandhumorallinksimmunityinpigletsundertheweaningcondition AT vitaliitkachuk correctionofcellularandhumorallinksimmunityinpigletsundertheweaningcondition AT nataliiamotko correctionofcellularandhumorallinksimmunityinpigletsundertheweaningcondition AT andriyboyko correctionofcellularandhumorallinksimmunityinpigletsundertheweaningcondition AT serhiipavkovych correctionofcellularandhumorallinksimmunityinpigletsundertheweaningcondition |