Molecular markers of cardiac fibrosis after myocardial infarction
Aim. To perform a screening for molecular markers of cardiac fibrosis upon myocardial infarction.Materials and Methods. We carried out echocardiography-guided endomyocardial biopsy of affected and intact interventricular septum segments of 7 patients with anterior myocardial infarction. Fibrotic and...
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| Format: | Article |
| Language: | Russian |
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Kemerovo State Medical University
2022-03-01
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| Series: | Фундаментальная и клиническая медицина |
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| Online Access: | https://fcm.kemsmu.ru/jour/article/view/511 |
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| author | O. L. Barbarash A. G. Kutikhin T. B. Pecherina R. S. Tarasov V. V. Kashtalap N. V. Fedorova L. A. Bogdanov O. N. Hryachkova D. Yu. Sedykh |
| author_facet | O. L. Barbarash A. G. Kutikhin T. B. Pecherina R. S. Tarasov V. V. Kashtalap N. V. Fedorova L. A. Bogdanov O. N. Hryachkova D. Yu. Sedykh |
| author_sort | O. L. Barbarash |
| collection | DOAJ |
| description | Aim. To perform a screening for molecular markers of cardiac fibrosis upon myocardial infarction.Materials and Methods. We carried out echocardiography-guided endomyocardial biopsy of affected and intact interventricular septum segments of 7 patients with anterior myocardial infarction. Fibrotic and adjacent intact cardiac tissue was dissected into 2 equal segments and: 1) homogenized with the further RNA extraction, reverse transcription, and quantitative polymerase chain reaction; 2) fixed in formalin and embedded into paraffin with the further van Gieson staining for the histological verification of cardiac fibrosis.Results. We found that the expression of ACTA2, VIM, CTGF, COL1A1, TGFB1, TGFBR1, AGTR1, CCL2 and TNF genes in fibrotic cardiac tissue was ≥ 3-fold higher as compared with the adjacent intact myocardium reflective of active extracellular matrix production by fibroblast-derived myofibroblasts.Conclusion. We have for the first time shown AGTR1, CCL2, and TNF genes as candidates for post-infarction cardiac fibrosis in addition to ACTA2, VIM, CTGF, COL1A1, TGFB1, and TGFBR1 genes. |
| format | Article |
| id | doaj-art-ea3c0f3aa08049028da34fb261808835 |
| institution | Kabale University |
| issn | 2500-0764 2542-0941 |
| language | Russian |
| publishDate | 2022-03-01 |
| publisher | Kemerovo State Medical University |
| record_format | Article |
| series | Фундаментальная и клиническая медицина |
| spelling | doaj-art-ea3c0f3aa08049028da34fb2618088352025-08-20T03:42:37ZrusKemerovo State Medical UniversityФундаментальная и клиническая медицина2500-07642542-09412022-03-0171173010.23946/2500-0764-2022-7-1-17-30300Molecular markers of cardiac fibrosis after myocardial infarctionO. L. Barbarash0A. G. Kutikhin1T. B. Pecherina2R. S. Tarasov3V. V. Kashtalap4N. V. Fedorova5L. A. Bogdanov6O. N. Hryachkova7D. Yu. Sedykh8Research Institute for Complex Issues of Cardiovascular DiseasesResearch Institute for Complex Issues of Cardiovascular DiseasesResearch Institute for Complex Issues of Cardiovascular DiseasesResearch Institute for Complex Issues of Cardiovascular DiseasesResearch Institute for Complex Issues of Cardiovascular DiseasesResearch Institute for Complex Issues of Cardiovascular DiseasesResearch Institute for Complex Issues of Cardiovascular DiseasesResearch Institute for Complex Issues of Cardiovascular DiseasesResearch Institute for Complex Issues of Cardiovascular DiseasesAim. To perform a screening for molecular markers of cardiac fibrosis upon myocardial infarction.Materials and Methods. We carried out echocardiography-guided endomyocardial biopsy of affected and intact interventricular septum segments of 7 patients with anterior myocardial infarction. Fibrotic and adjacent intact cardiac tissue was dissected into 2 equal segments and: 1) homogenized with the further RNA extraction, reverse transcription, and quantitative polymerase chain reaction; 2) fixed in formalin and embedded into paraffin with the further van Gieson staining for the histological verification of cardiac fibrosis.Results. We found that the expression of ACTA2, VIM, CTGF, COL1A1, TGFB1, TGFBR1, AGTR1, CCL2 and TNF genes in fibrotic cardiac tissue was ≥ 3-fold higher as compared with the adjacent intact myocardium reflective of active extracellular matrix production by fibroblast-derived myofibroblasts.Conclusion. We have for the first time shown AGTR1, CCL2, and TNF genes as candidates for post-infarction cardiac fibrosis in addition to ACTA2, VIM, CTGF, COL1A1, TGFB1, and TGFBR1 genes.https://fcm.kemsmu.ru/jour/article/view/511myocardial infarctioncardiac fibrosismolecular markersagtr1ccl2tnf |
| spellingShingle | O. L. Barbarash A. G. Kutikhin T. B. Pecherina R. S. Tarasov V. V. Kashtalap N. V. Fedorova L. A. Bogdanov O. N. Hryachkova D. Yu. Sedykh Molecular markers of cardiac fibrosis after myocardial infarction Фундаментальная и клиническая медицина myocardial infarction cardiac fibrosis molecular markers agtr1 ccl2 tnf |
| title | Molecular markers of cardiac fibrosis after myocardial infarction |
| title_full | Molecular markers of cardiac fibrosis after myocardial infarction |
| title_fullStr | Molecular markers of cardiac fibrosis after myocardial infarction |
| title_full_unstemmed | Molecular markers of cardiac fibrosis after myocardial infarction |
| title_short | Molecular markers of cardiac fibrosis after myocardial infarction |
| title_sort | molecular markers of cardiac fibrosis after myocardial infarction |
| topic | myocardial infarction cardiac fibrosis molecular markers agtr1 ccl2 tnf |
| url | https://fcm.kemsmu.ru/jour/article/view/511 |
| work_keys_str_mv | AT olbarbarash molecularmarkersofcardiacfibrosisaftermyocardialinfarction AT agkutikhin molecularmarkersofcardiacfibrosisaftermyocardialinfarction AT tbpecherina molecularmarkersofcardiacfibrosisaftermyocardialinfarction AT rstarasov molecularmarkersofcardiacfibrosisaftermyocardialinfarction AT vvkashtalap molecularmarkersofcardiacfibrosisaftermyocardialinfarction AT nvfedorova molecularmarkersofcardiacfibrosisaftermyocardialinfarction AT labogdanov molecularmarkersofcardiacfibrosisaftermyocardialinfarction AT onhryachkova molecularmarkersofcardiacfibrosisaftermyocardialinfarction AT dyusedykh molecularmarkersofcardiacfibrosisaftermyocardialinfarction |