Sammenligning av metoder for eluering av IgG-antistoff bundet til erytrocytter
Background: IgG antibodies bound to erythrocytes may be involved in various disease processes. Identification of these antibodies and antigen typing of patients with a positive direct antiglobulin (DAT) test is relevant both for transfusion therapy and follow-up of patients during pregnancy. We have...
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NITO - Bioingeniørfaglig institutt
2024-10-01
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| Series: | Bioingeniøren |
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| Online Access: | https://forskning.bioingenioren.no/originalartikkel/sammenligning-av-metoder-for-eluering-av-igg-antistoff-bundet-til-erytrocytter/116303 |
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| _version_ | 1841553418441719808 |
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| author | Elin Hallheim Reiersøl Thomas E. Hundhausen |
| author_facet | Elin Hallheim Reiersøl Thomas E. Hundhausen |
| author_sort | Elin Hallheim Reiersøl |
| collection | DOAJ |
| description | Background: IgG antibodies bound to erythrocytes may be involved in various disease processes. Identification of these antibodies and antigen typing of patients with a positive direct antiglobulin (DAT) test is relevant both for transfusion therapy and follow-up of patients during pregnancy. We have compared elution efficiency and concordance of results for various CE (Communauté Européenne) and IVD (In vitro Diagnostic) labelled methods and heat elution.
Method: Elution methods for stripping of DAT-positive erythrocytes and for detection of antibodies in eluate were compared. McNemars test was used to compare the effectiveness of the methods. P-values < 0,05 were considered statistically significant and Holm-Bonferronis method for multiple comparisons was used. The proportion of matching results between the methods was calculated as a percentage.
Results: We demonstrated significant differences in diagnostic sensitivity between the different methods. Concordance of results between methods ranged from 64 to 90 %. In antigen typing of DAT-negative erythrocytes after antibody stripping, both false negative and false positive results were observed.
Conclusion: Methods for elution of IgG antibodies from erythrocytes should be included in routine investigations for certain problems. Different methods do not provide consistent results for all problems; therefore, alternative methods should be available.
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| format | Article |
| id | doaj-art-ea362c00e05d46c9944e998e742004fc |
| institution | Kabale University |
| issn | 0801-6828 1890-1875 |
| language | Danish |
| publishDate | 2024-10-01 |
| publisher | NITO - Bioingeniørfaglig institutt |
| record_format | Article |
| series | Bioingeniøren |
| spelling | doaj-art-ea362c00e05d46c9944e998e742004fc2025-01-09T08:45:06ZdanNITO - Bioingeniørfaglig instituttBioingeniøren0801-68281890-18752024-10-015971622Sammenligning av metoder for eluering av IgG-antistoff bundet til erytrocytterElin Hallheim Reiersøl0Thomas E. Hundhausen1 Sørlandet Sykehus HF Sørlandet Sykehus HF; Universitetet i Agder, Institutt for naturvitenskapBackground: IgG antibodies bound to erythrocytes may be involved in various disease processes. Identification of these antibodies and antigen typing of patients with a positive direct antiglobulin (DAT) test is relevant both for transfusion therapy and follow-up of patients during pregnancy. We have compared elution efficiency and concordance of results for various CE (Communauté Européenne) and IVD (In vitro Diagnostic) labelled methods and heat elution. Method: Elution methods for stripping of DAT-positive erythrocytes and for detection of antibodies in eluate were compared. McNemars test was used to compare the effectiveness of the methods. P-values < 0,05 were considered statistically significant and Holm-Bonferronis method for multiple comparisons was used. The proportion of matching results between the methods was calculated as a percentage. Results: We demonstrated significant differences in diagnostic sensitivity between the different methods. Concordance of results between methods ranged from 64 to 90 %. In antigen typing of DAT-negative erythrocytes after antibody stripping, both false negative and false positive results were observed. Conclusion: Methods for elution of IgG antibodies from erythrocytes should be included in routine investigations for certain problems. Different methods do not provide consistent results for all problems; therefore, alternative methods should be available. https://forskning.bioingenioren.no/originalartikkel/sammenligning-av-metoder-for-eluering-av-igg-antistoff-bundet-til-erytrocytter/116303igg-antibodyerythrocyteselutiondirect antiglobulin testdat |
| spellingShingle | Elin Hallheim Reiersøl Thomas E. Hundhausen Sammenligning av metoder for eluering av IgG-antistoff bundet til erytrocytter Bioingeniøren igg-antibody erythrocytes elution direct antiglobulin test dat |
| title | Sammenligning av metoder for eluering av IgG-antistoff bundet til erytrocytter |
| title_full | Sammenligning av metoder for eluering av IgG-antistoff bundet til erytrocytter |
| title_fullStr | Sammenligning av metoder for eluering av IgG-antistoff bundet til erytrocytter |
| title_full_unstemmed | Sammenligning av metoder for eluering av IgG-antistoff bundet til erytrocytter |
| title_short | Sammenligning av metoder for eluering av IgG-antistoff bundet til erytrocytter |
| title_sort | sammenligning av metoder for eluering av igg antistoff bundet til erytrocytter |
| topic | igg-antibody erythrocytes elution direct antiglobulin test dat |
| url | https://forskning.bioingenioren.no/originalartikkel/sammenligning-av-metoder-for-eluering-av-igg-antistoff-bundet-til-erytrocytter/116303 |
| work_keys_str_mv | AT elinhallheimreiersøl sammenligningavmetoderforelueringaviggantistoffbundettilerytrocytter AT thomasehundhausen sammenligningavmetoderforelueringaviggantistoffbundettilerytrocytter |