A simple, sensitive method for the simultaneous determination of cotinine and trans-3´-hydroxycotinine in human serum by LC-MS/MS

Abstract A simple and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the simultaneous determination of cotinine and its metabolite, trans-3´-hydroxycotinine, in human serum. Liquid–liquid extraction (LLE) using dichloromethane (DCM) and eth...

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Bibliographic Details
Main Authors: Xiujiao Joanne Ye, Amy M. MacDonald, Melissa J. Bennett, David W. Kinniburgh
Format: Article
Language:English
Published: SpringerOpen 2025-05-01
Series:Journal of Analytical Science and Technology
Subjects:
Online Access:https://doi.org/10.1186/s40543-025-00488-y
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