Equivalent Circuit Modeling and Analysis for Microfluidic Electrical Impedance Monitoring of Single-Cell Growth
Microfluidics has significantly advanced the field of single-cell analysis, particularly in studies related to cell growth, division, and heterogeneity. Electrical impedance spectroscopy (EIS), a label-free and non-invasive biosensing technique, has been integrated into microfluidic devices for high...
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MDPI AG
2025-02-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/15/2/113 |
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| author | Yingying Wang Haoran Wu Yulu Geng Zhao Zhang Jiaming Fu Jia Ouyang Zhen Zhu |
| author_facet | Yingying Wang Haoran Wu Yulu Geng Zhao Zhang Jiaming Fu Jia Ouyang Zhen Zhu |
| author_sort | Yingying Wang |
| collection | DOAJ |
| description | Microfluidics has significantly advanced the field of single-cell analysis, particularly in studies related to cell growth, division, and heterogeneity. Electrical impedance spectroscopy (EIS), a label-free and non-invasive biosensing technique, has been integrated into microfluidic devices for high-throughput and long-term monitoring of single budding yeast cells. Accurate interpretation of EIS measurements of cell growth dynamics necessitates the establishment of theoretical equivalent circuit models for the single-cell sensing system. Here, we report on the development of equivalent circuit models of an in situ EIS sensing system to elucidate cell growth. Firstly, finite element modeling and simulation of an EIS measurement of cell growth in the EIS sensing unit were performed, guiding the fittings of electrical components for an established equivalent circuit model (ECM). From the ECM, we extracted an equivalent volume fraction applicable to various cell and sensing unit geometries to describe the geometry-dependent sensing characteristics corresponding to the electrical response in the model. Then, EIS measurements of an immobilized cell in a microfluidic device were conducted via peripheral circuits. A lumped parameter model for the entire EIS measurement system was established, with electrical components determined by fitting to experimental data. The rationality of the proposed theoretical model was validated through the long-term impedance variation induced by cell growth in experiments, demonstrating its feasibility in linking EIS data with the bio-physics underlying the experimental phenomenon. |
| format | Article |
| id | doaj-art-ea276c31d5a14313a1bfbcbb2ed3073c |
| institution | DOAJ |
| issn | 2079-6374 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj-art-ea276c31d5a14313a1bfbcbb2ed3073c2025-08-20T03:12:14ZengMDPI AGBiosensors2079-63742025-02-0115211310.3390/bios15020113Equivalent Circuit Modeling and Analysis for Microfluidic Electrical Impedance Monitoring of Single-Cell GrowthYingying Wang0Haoran Wu1Yulu Geng2Zhao Zhang3Jiaming Fu4Jia Ouyang5Zhen Zhu6School of Integrated Circuits, Southeast University, Wuxi Campus, Zhuangyuan Road 5, Wuxi 214000, ChinaSchool of Integrated Circuits, Southeast University, Wuxi Campus, Zhuangyuan Road 5, Wuxi 214000, ChinaSchool of Integrated Circuits, Southeast University, Wuxi Campus, Zhuangyuan Road 5, Wuxi 214000, ChinaSchool of Integrated Circuits, Southeast University, Wuxi Campus, Zhuangyuan Road 5, Wuxi 214000, ChinaCollege of Chemical Engineering, Nanjing Forestry University, Longpan Road 159, Nanjing 210037, ChinaCollege of Chemical Engineering, Nanjing Forestry University, Longpan Road 159, Nanjing 210037, ChinaSchool of Integrated Circuits, Southeast University, Wuxi Campus, Zhuangyuan Road 5, Wuxi 214000, ChinaMicrofluidics has significantly advanced the field of single-cell analysis, particularly in studies related to cell growth, division, and heterogeneity. Electrical impedance spectroscopy (EIS), a label-free and non-invasive biosensing technique, has been integrated into microfluidic devices for high-throughput and long-term monitoring of single budding yeast cells. Accurate interpretation of EIS measurements of cell growth dynamics necessitates the establishment of theoretical equivalent circuit models for the single-cell sensing system. Here, we report on the development of equivalent circuit models of an in situ EIS sensing system to elucidate cell growth. Firstly, finite element modeling and simulation of an EIS measurement of cell growth in the EIS sensing unit were performed, guiding the fittings of electrical components for an established equivalent circuit model (ECM). From the ECM, we extracted an equivalent volume fraction applicable to various cell and sensing unit geometries to describe the geometry-dependent sensing characteristics corresponding to the electrical response in the model. Then, EIS measurements of an immobilized cell in a microfluidic device were conducted via peripheral circuits. A lumped parameter model for the entire EIS measurement system was established, with electrical components determined by fitting to experimental data. The rationality of the proposed theoretical model was validated through the long-term impedance variation induced by cell growth in experiments, demonstrating its feasibility in linking EIS data with the bio-physics underlying the experimental phenomenon.https://www.mdpi.com/2079-6374/15/2/113electrical impedance spectroscopyequivalent circuit modelmicroelectrode array<i>Saccharomyces cerevisiae</i> |
| spellingShingle | Yingying Wang Haoran Wu Yulu Geng Zhao Zhang Jiaming Fu Jia Ouyang Zhen Zhu Equivalent Circuit Modeling and Analysis for Microfluidic Electrical Impedance Monitoring of Single-Cell Growth Biosensors electrical impedance spectroscopy equivalent circuit model microelectrode array <i>Saccharomyces cerevisiae</i> |
| title | Equivalent Circuit Modeling and Analysis for Microfluidic Electrical Impedance Monitoring of Single-Cell Growth |
| title_full | Equivalent Circuit Modeling and Analysis for Microfluidic Electrical Impedance Monitoring of Single-Cell Growth |
| title_fullStr | Equivalent Circuit Modeling and Analysis for Microfluidic Electrical Impedance Monitoring of Single-Cell Growth |
| title_full_unstemmed | Equivalent Circuit Modeling and Analysis for Microfluidic Electrical Impedance Monitoring of Single-Cell Growth |
| title_short | Equivalent Circuit Modeling and Analysis for Microfluidic Electrical Impedance Monitoring of Single-Cell Growth |
| title_sort | equivalent circuit modeling and analysis for microfluidic electrical impedance monitoring of single cell growth |
| topic | electrical impedance spectroscopy equivalent circuit model microelectrode array <i>Saccharomyces cerevisiae</i> |
| url | https://www.mdpi.com/2079-6374/15/2/113 |
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