Investigation of tomato ringspot virus (ToRSV) by Real-Time TaqMan RT-PCR in Hakkari province, Turkey
Tomato ringspot virus (ToRSV) belongs to the Nepovirus genus in the family Secoviridae. It has a wide host range and is listed as a quarantine virus in Turkey. In this study, 80 leaf samples were collected from tomato, pepper, cucumber and grapevine cultivation sites located in three different parts...
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Ankara University
2021-09-01
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| Series: | Journal of Agricultural Sciences |
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| Online Access: | https://dergipark.org.tr/tr/download/article-file/938033 |
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| author | Nevin Akdura Murat Şevik |
| author_facet | Nevin Akdura Murat Şevik |
| author_sort | Nevin Akdura |
| collection | DOAJ |
| description | Tomato ringspot virus (ToRSV) belongs to the Nepovirus genus in the family Secoviridae. It has a wide host range and is listed as a quarantine virus in Turkey. In this study, 80 leaf samples were collected from tomato, pepper, cucumber and grapevine cultivation sites located in three different parts of Hakkari province: Şemdinli, Çukurca and Center districts. Real-time TaqMan reverse transcription-polymerase chain reaction (RT-PCR) method was used for the detection of the virus. Amplification was carried out in reaction mix including QuantiNova Probe RT-PCR kit (Qiagen, Germany) using primers and TaqMan probe based on 3'-UTR (untranslated region) of virus, which amplified a 182 bp product of the genome. ToRSV was detected in 13 of the 80 samples and threshold cycle (CT) values ranged from 23.9 to 37.4. It was found that 16.25% of the samples collected from the districts of Hakkari province were found to be infected with ToRSV whereas no ToRSV was detected in the samples collected from the center of the city. The virus was detected on pepper and cucumber samples in Çukurca district, and it was also detected in tomato, pepper, cucumber and grapevine samples in Şemdinli district. To our knowledge, this study is the first report of molecular detection of ToRSV by real-time TaqMan RT-PCR in Turkey. |
| format | Article |
| id | doaj-art-ea217b2968794cc0b4427029a52d23fe |
| institution | Kabale University |
| issn | 1300-7580 2148-9297 |
| language | English |
| publishDate | 2021-09-01 |
| publisher | Ankara University |
| record_format | Article |
| series | Journal of Agricultural Sciences |
| spelling | doaj-art-ea217b2968794cc0b4427029a52d23fe2025-08-20T03:26:34ZengAnkara UniversityJournal of Agricultural Sciences1300-75802148-92972021-09-0127333534010.15832/ankutbd.67771045Investigation of tomato ringspot virus (ToRSV) by Real-Time TaqMan RT-PCR in Hakkari province, TurkeyNevin Akdura0Murat Şevik1HAKKARI UNIVERSITYHATAY MUSTAFA KEMAL ÜNİVERSİTESİTomato ringspot virus (ToRSV) belongs to the Nepovirus genus in the family Secoviridae. It has a wide host range and is listed as a quarantine virus in Turkey. In this study, 80 leaf samples were collected from tomato, pepper, cucumber and grapevine cultivation sites located in three different parts of Hakkari province: Şemdinli, Çukurca and Center districts. Real-time TaqMan reverse transcription-polymerase chain reaction (RT-PCR) method was used for the detection of the virus. Amplification was carried out in reaction mix including QuantiNova Probe RT-PCR kit (Qiagen, Germany) using primers and TaqMan probe based on 3'-UTR (untranslated region) of virus, which amplified a 182 bp product of the genome. ToRSV was detected in 13 of the 80 samples and threshold cycle (CT) values ranged from 23.9 to 37.4. It was found that 16.25% of the samples collected from the districts of Hakkari province were found to be infected with ToRSV whereas no ToRSV was detected in the samples collected from the center of the city. The virus was detected on pepper and cucumber samples in Çukurca district, and it was also detected in tomato, pepper, cucumber and grapevine samples in Şemdinli district. To our knowledge, this study is the first report of molecular detection of ToRSV by real-time TaqMan RT-PCR in Turkey.https://dergipark.org.tr/tr/download/article-file/938033torsvmolecular detectiontomatopeppercucumbergrapevineturkey |
| spellingShingle | Nevin Akdura Murat Şevik Investigation of tomato ringspot virus (ToRSV) by Real-Time TaqMan RT-PCR in Hakkari province, Turkey Journal of Agricultural Sciences torsv molecular detection tomato pepper cucumber grapevine turkey |
| title | Investigation of tomato ringspot virus (ToRSV) by Real-Time TaqMan RT-PCR in Hakkari province, Turkey |
| title_full | Investigation of tomato ringspot virus (ToRSV) by Real-Time TaqMan RT-PCR in Hakkari province, Turkey |
| title_fullStr | Investigation of tomato ringspot virus (ToRSV) by Real-Time TaqMan RT-PCR in Hakkari province, Turkey |
| title_full_unstemmed | Investigation of tomato ringspot virus (ToRSV) by Real-Time TaqMan RT-PCR in Hakkari province, Turkey |
| title_short | Investigation of tomato ringspot virus (ToRSV) by Real-Time TaqMan RT-PCR in Hakkari province, Turkey |
| title_sort | investigation of tomato ringspot virus torsv by real time taqman rt pcr in hakkari province turkey |
| topic | torsv molecular detection tomato pepper cucumber grapevine turkey |
| url | https://dergipark.org.tr/tr/download/article-file/938033 |
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