Association of chronic periodontitis and type 2 diabetes mellitus with salivary Del-1 and IL-17 levels
Purpose: Chronic periodontitis (CP) and Type 2 diabetes mellitus (T2DM) are chronic diseases affecting the humans and have interrelationship in their pathogenesis. Monitoring the progress with biomarker in these disease is important from treatment outcome point of view. We investigated the associati...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-10-01
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| Series: | Journal of Oral Biology and Craniofacial Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212426820301287 |
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| author | Somil Saxena Ranganath Venugopal Rachana Chandrayan Rao Monal B. Yuwanati Harshita Awasthi Megha Jain |
| author_facet | Somil Saxena Ranganath Venugopal Rachana Chandrayan Rao Monal B. Yuwanati Harshita Awasthi Megha Jain |
| author_sort | Somil Saxena |
| collection | DOAJ |
| description | Purpose: Chronic periodontitis (CP) and Type 2 diabetes mellitus (T2DM) are chronic diseases affecting the humans and have interrelationship in their pathogenesis. Monitoring the progress with biomarker in these disease is important from treatment outcome point of view. We investigated the association of salivary developmental endothelial locus-1 (Del-1) and interleukin-17 (IL-17) levels in CP and T2DM. Methods: Saliva was collected from 17 healthy and 68 patients (divided into 4 groups based on presence or absence of CP and T2DM). Periodontal parameters were recorded at the time of visit. Del-1 and IL-17 levels in unstimulated salivary samples were determined using enzyme-linked immunosorbent assay. Results: Study found the lower levels of salivary Del-1 and higher levels of IL-17 in CP (2.23 ± 2.10 ng/mL, 56.54 ± 19.79 ng/mL), CCDM ((1.97 ± 1.36 ng/mL, 74.74 ± 17.79 ng/mL) and CUDM (1.02 ± 0.52 ng/mL, 63.84 ± 24.72 ng/mL) as compared to healthy individuals (6.54 ± 2.07; 24.47 ± 8.23 ng/mL) (p < 0.001). Del-1 and IL-17 levels were correlating with inflammatory state in CP and presence of T2DM aggravate the severity of disease in CP. An inverse correlation between salivary Del-1 & IL-17 levels was also seen. Furthermore, we also observed that the combination of T2DM and CP (CCDM and CUDM) increases the salivary IL-17 levels and decreases Del-1 levels. Conclusions: There is an upregulation of salivary IL-17 and downregulation of salivary Del-1 with increase in severity of periodontal disease as well as T2DM. Furthermore, the presence of T2DM in chronic periodontitis patients can aggravate the inflammation related periodontal destruction. |
| format | Article |
| id | doaj-art-ea1cd491f0154ac89ef157896854e9a5 |
| institution | OA Journals |
| issn | 2212-4268 |
| language | English |
| publishDate | 2020-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Oral Biology and Craniofacial Research |
| spelling | doaj-art-ea1cd491f0154ac89ef157896854e9a52025-08-20T02:02:02ZengElsevierJournal of Oral Biology and Craniofacial Research2212-42682020-10-0110452953410.1016/j.jobcr.2020.08.013Association of chronic periodontitis and type 2 diabetes mellitus with salivary Del-1 and IL-17 levelsSomil Saxena0Ranganath Venugopal1Rachana Chandrayan Rao2Monal B. Yuwanati3Harshita Awasthi4Megha Jain5People's College of Dental Sciences and Research Centre, People's University, Bhopal, IndiaAECS Maaruti College of Dental Sciences and Research Centre, Bangalore, IndiaAECS Maaruti College of Dental Sciences and Research Centre, Bangalore, IndiaPeople's College of Dental Sciences and Research Centre, People's University, Bhopal, India; Corresponding author. Department of Oral Pathology and Microbiology, People's College of Dental Science and Research Centre, Bhanpur, Bhopal, 462037, India.AECS Maaruti College of Dental Sciences and Research Centre, Bangalore, IndiaPeople's College of Dental Sciences and Research Centre, People's University, Bhopal, IndiaPurpose: Chronic periodontitis (CP) and Type 2 diabetes mellitus (T2DM) are chronic diseases affecting the humans and have interrelationship in their pathogenesis. Monitoring the progress with biomarker in these disease is important from treatment outcome point of view. We investigated the association of salivary developmental endothelial locus-1 (Del-1) and interleukin-17 (IL-17) levels in CP and T2DM. Methods: Saliva was collected from 17 healthy and 68 patients (divided into 4 groups based on presence or absence of CP and T2DM). Periodontal parameters were recorded at the time of visit. Del-1 and IL-17 levels in unstimulated salivary samples were determined using enzyme-linked immunosorbent assay. Results: Study found the lower levels of salivary Del-1 and higher levels of IL-17 in CP (2.23 ± 2.10 ng/mL, 56.54 ± 19.79 ng/mL), CCDM ((1.97 ± 1.36 ng/mL, 74.74 ± 17.79 ng/mL) and CUDM (1.02 ± 0.52 ng/mL, 63.84 ± 24.72 ng/mL) as compared to healthy individuals (6.54 ± 2.07; 24.47 ± 8.23 ng/mL) (p < 0.001). Del-1 and IL-17 levels were correlating with inflammatory state in CP and presence of T2DM aggravate the severity of disease in CP. An inverse correlation between salivary Del-1 & IL-17 levels was also seen. Furthermore, we also observed that the combination of T2DM and CP (CCDM and CUDM) increases the salivary IL-17 levels and decreases Del-1 levels. Conclusions: There is an upregulation of salivary IL-17 and downregulation of salivary Del-1 with increase in severity of periodontal disease as well as T2DM. Furthermore, the presence of T2DM in chronic periodontitis patients can aggravate the inflammation related periodontal destruction.http://www.sciencedirect.com/science/article/pii/S2212426820301287Salivary biomarkerChronic periodontitisDevelopmental endothelial locusl-1 proteinInterleukin-17Type 2 Diabetes mellitus |
| spellingShingle | Somil Saxena Ranganath Venugopal Rachana Chandrayan Rao Monal B. Yuwanati Harshita Awasthi Megha Jain Association of chronic periodontitis and type 2 diabetes mellitus with salivary Del-1 and IL-17 levels Journal of Oral Biology and Craniofacial Research Salivary biomarker Chronic periodontitis Developmental endothelial locusl-1 protein Interleukin-17 Type 2 Diabetes mellitus |
| title | Association of chronic periodontitis and type 2 diabetes mellitus with salivary Del-1 and IL-17 levels |
| title_full | Association of chronic periodontitis and type 2 diabetes mellitus with salivary Del-1 and IL-17 levels |
| title_fullStr | Association of chronic periodontitis and type 2 diabetes mellitus with salivary Del-1 and IL-17 levels |
| title_full_unstemmed | Association of chronic periodontitis and type 2 diabetes mellitus with salivary Del-1 and IL-17 levels |
| title_short | Association of chronic periodontitis and type 2 diabetes mellitus with salivary Del-1 and IL-17 levels |
| title_sort | association of chronic periodontitis and type 2 diabetes mellitus with salivary del 1 and il 17 levels |
| topic | Salivary biomarker Chronic periodontitis Developmental endothelial locusl-1 protein Interleukin-17 Type 2 Diabetes mellitus |
| url | http://www.sciencedirect.com/science/article/pii/S2212426820301287 |
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