Determination of expression level of AP1S1, CDK9, FIGF and HDAC11 genes in bladder tumors for aggressive phenotype characterization
Bladder cancer holds a steady 10th place among oncological diseases. Follow-up and timely diagnosis of recurrence and progression of bladder cancer are still based on regular but outdated cystoscopy followed by cytological examination. To reduce the number of cystoscopy procedures, new and reliable...
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Taylor & Francis Group
2023-03-01
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| Series: | Biotechnology & Biotechnological Equipment |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/13102818.2023.2253926 |
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| author | Olga Antonova Zora Hammoudeh Zornitza Yordanova Boris Mladenov |
| author_facet | Olga Antonova Zora Hammoudeh Zornitza Yordanova Boris Mladenov |
| author_sort | Olga Antonova |
| collection | DOAJ |
| description | Bladder cancer holds a steady 10th place among oncological diseases. Follow-up and timely diagnosis of recurrence and progression of bladder cancer are still based on regular but outdated cystoscopy followed by cytological examination. To reduce the number of cystoscopy procedures, new and reliable biomarkers for predicting tumor behavior must be developed. The aim of this study was to confirm our previous results that demonstrated overexpression of AP1S1, CDK9, FIGF and HDAC11 in muscle-invasive bladder cancer. The project management was performed using iterative flexible project work (Flexible Methodology for Innovative Projects in Scientific Organizations, FMIPSO). Gene expression analysis of the AP1S1, CDK9, FIGF and HDAC11 genes was evaluated in 39 newly collected non-invasive and muscle-invasive bladder tumors. Differential gene expression was calculated using the ΔΔCt method with GPDH as a housekeeping gene. The 4,0-fold change in gene expression was used as a cutoff to determine up- or down-regulation compared to the negative control. Our results demonstrate the involvement of the FIGF, CDK9 and HDAC11 in tumor progression and their potential use as candidate biomarkers to characterize invasive tumor phenotype and muscle progression, as well as potentially reduce the number of cystoscopies. We used FMIPSO to be able to achieve the results at the optimum level of efficiency and control of the project with all possible constraints in scientific organizations. |
| format | Article |
| id | doaj-art-ea19be861b35490a8e4aa47bfe8c60ff |
| institution | OA Journals |
| issn | 1310-2818 1314-3530 |
| language | English |
| publishDate | 2023-03-01 |
| publisher | Taylor & Francis Group |
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| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj-art-ea19be861b35490a8e4aa47bfe8c60ff2025-08-20T02:23:41ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302023-03-0137110.1080/13102818.2023.2253926Determination of expression level of AP1S1, CDK9, FIGF and HDAC11 genes in bladder tumors for aggressive phenotype characterizationOlga Antonova0Zora Hammoudeh1Zornitza Yordanova2Boris Mladenov3Department of Medical Genetics, Medical Faculty, Medical University of Sofia, Sofia, BulgariaDepartment of Medical Genetics, Medical Faculty, Medical University of Sofia, Sofia, BulgariaIndustrial Business Department, Business Faculty, University of National and World Economy, Sofia, BulgariaThird Clinic, Department of Urology, University Hospital UMBALSM “N. I. Pirogov”, Sofia, BulgariaBladder cancer holds a steady 10th place among oncological diseases. Follow-up and timely diagnosis of recurrence and progression of bladder cancer are still based on regular but outdated cystoscopy followed by cytological examination. To reduce the number of cystoscopy procedures, new and reliable biomarkers for predicting tumor behavior must be developed. The aim of this study was to confirm our previous results that demonstrated overexpression of AP1S1, CDK9, FIGF and HDAC11 in muscle-invasive bladder cancer. The project management was performed using iterative flexible project work (Flexible Methodology for Innovative Projects in Scientific Organizations, FMIPSO). Gene expression analysis of the AP1S1, CDK9, FIGF and HDAC11 genes was evaluated in 39 newly collected non-invasive and muscle-invasive bladder tumors. Differential gene expression was calculated using the ΔΔCt method with GPDH as a housekeeping gene. The 4,0-fold change in gene expression was used as a cutoff to determine up- or down-regulation compared to the negative control. Our results demonstrate the involvement of the FIGF, CDK9 and HDAC11 in tumor progression and their potential use as candidate biomarkers to characterize invasive tumor phenotype and muscle progression, as well as potentially reduce the number of cystoscopies. We used FMIPSO to be able to achieve the results at the optimum level of efficiency and control of the project with all possible constraints in scientific organizations.https://www.tandfonline.com/doi/10.1080/13102818.2023.2253926Bladder cancerinvasionbiomarkergene expression |
| spellingShingle | Olga Antonova Zora Hammoudeh Zornitza Yordanova Boris Mladenov Determination of expression level of AP1S1, CDK9, FIGF and HDAC11 genes in bladder tumors for aggressive phenotype characterization Biotechnology & Biotechnological Equipment Bladder cancer invasion biomarker gene expression |
| title | Determination of expression level of AP1S1, CDK9, FIGF and HDAC11 genes in bladder tumors for aggressive phenotype characterization |
| title_full | Determination of expression level of AP1S1, CDK9, FIGF and HDAC11 genes in bladder tumors for aggressive phenotype characterization |
| title_fullStr | Determination of expression level of AP1S1, CDK9, FIGF and HDAC11 genes in bladder tumors for aggressive phenotype characterization |
| title_full_unstemmed | Determination of expression level of AP1S1, CDK9, FIGF and HDAC11 genes in bladder tumors for aggressive phenotype characterization |
| title_short | Determination of expression level of AP1S1, CDK9, FIGF and HDAC11 genes in bladder tumors for aggressive phenotype characterization |
| title_sort | determination of expression level of ap1s1 cdk9 figf and hdac11 genes in bladder tumors for aggressive phenotype characterization |
| topic | Bladder cancer invasion biomarker gene expression |
| url | https://www.tandfonline.com/doi/10.1080/13102818.2023.2253926 |
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