Genome-wide identification and expression profiling of the bZIP gene family in Chrysanthemum indicum and the functional analysis of CibZIP29 under cadmium stress
Recent industrial activities have increased cadmium (Cd) contamination in soils, negatively affecting crop growth and agricultural development. Chrysanthemum indicum represents a significant wild species within the Chrysanthemum genus. Its distinctive resistance and adaptability serve as valuable re...
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Maximum Academic Press
2025-01-01
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| Series: | Ornamental Plant Research |
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| Online Access: | https://www.maxapress.com/article/doi/10.48130/opr-0025-0010 |
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| author | Shengyan Chen Yin Zhang Kaiyuan Zhang Shuguang Liu Yujia Yang Xingnong Xu |
| author_facet | Shengyan Chen Yin Zhang Kaiyuan Zhang Shuguang Liu Yujia Yang Xingnong Xu |
| author_sort | Shengyan Chen |
| collection | DOAJ |
| description | Recent industrial activities have increased cadmium (Cd) contamination in soils, negatively affecting crop growth and agricultural development. Chrysanthemum indicum represents a significant wild species within the Chrysanthemum genus. Its distinctive resistance and adaptability serve as valuable references for the enhancement of ornamental chrysanthemum breeding programs. However, the role of bZIP family of Cd stress in C. indicum remains unclear. This study conducted a genome-wide identification and expression analysis of bZIP genes in C. indicum, identifying 65 CibZIP genes distributed across nine chromosomes. Phylogenetic analysis grouped them into 12 subgroups, supported by conserved motifs, gene, and protein structure analyses. Tandem and segmental duplications were found to be key drivers of CibZIP gene family expansion, with Ka/Ks analysis indicating purifying selection of duplicated genes. Promoter analysis revealed cis-acting elements involved in plant development and stress response. Transcriptomic data showed that CibZIP genes are highly expressed in different tissues and play significant roles in Cd stress tolerance. RT-qPCR analysis further confirmed that specific CibZIP genes, especially CibZIP29, are upregulated in response to Cd stress. Overexpression of CibZIP29 in transgenic Arabidopsis enhanced Cd tolerance, providing new insights into the molecular mechanisms underlying Cd stress response in C. indicum. These findings contribute to understanding the role of CibZIP genes in Cd tolerance and will support future research on Cd stress mechanisms in Chrysanthemum. |
| format | Article |
| id | doaj-art-ea183799fa0c4bd786910b9d1c3c5afc |
| institution | OA Journals |
| issn | 2769-2094 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Maximum Academic Press |
| record_format | Article |
| series | Ornamental Plant Research |
| spelling | doaj-art-ea183799fa0c4bd786910b9d1c3c5afc2025-08-20T02:12:33ZengMaximum Academic PressOrnamental Plant Research2769-20942025-01-015111710.48130/opr-0025-0010opr-0025-0010Genome-wide identification and expression profiling of the bZIP gene family in Chrysanthemum indicum and the functional analysis of CibZIP29 under cadmium stressShengyan Chen0Yin Zhang1Kaiyuan Zhang2Shuguang Liu3Yujia Yang4Xingnong Xu5College of Landscape Architecture, Northeast Forestry University, Haerbin 150040, Heilongjiang, ChinaCollege of Landscape Architecture, Northeast Forestry University, Haerbin 150040, Heilongjiang, ChinaCollege of Landscape Architecture, Northeast Forestry University, Haerbin 150040, Heilongjiang, ChinaCollege of Landscape Architecture, Northeast Forestry University, Haerbin 150040, Heilongjiang, ChinaCollege of Landscape Architecture, Northeast Forestry University, Haerbin 150040, Heilongjiang, ChinaYancheng Third People's Hospital, Yancheng 224008, Jiangsu, ChinaRecent industrial activities have increased cadmium (Cd) contamination in soils, negatively affecting crop growth and agricultural development. Chrysanthemum indicum represents a significant wild species within the Chrysanthemum genus. Its distinctive resistance and adaptability serve as valuable references for the enhancement of ornamental chrysanthemum breeding programs. However, the role of bZIP family of Cd stress in C. indicum remains unclear. This study conducted a genome-wide identification and expression analysis of bZIP genes in C. indicum, identifying 65 CibZIP genes distributed across nine chromosomes. Phylogenetic analysis grouped them into 12 subgroups, supported by conserved motifs, gene, and protein structure analyses. Tandem and segmental duplications were found to be key drivers of CibZIP gene family expansion, with Ka/Ks analysis indicating purifying selection of duplicated genes. Promoter analysis revealed cis-acting elements involved in plant development and stress response. Transcriptomic data showed that CibZIP genes are highly expressed in different tissues and play significant roles in Cd stress tolerance. RT-qPCR analysis further confirmed that specific CibZIP genes, especially CibZIP29, are upregulated in response to Cd stress. Overexpression of CibZIP29 in transgenic Arabidopsis enhanced Cd tolerance, providing new insights into the molecular mechanisms underlying Cd stress response in C. indicum. These findings contribute to understanding the role of CibZIP genes in Cd tolerance and will support future research on Cd stress mechanisms in Chrysanthemum.https://www.maxapress.com/article/doi/10.48130/opr-0025-0010chrysanthemum indicumbzipscd stresscibzip29 |
| spellingShingle | Shengyan Chen Yin Zhang Kaiyuan Zhang Shuguang Liu Yujia Yang Xingnong Xu Genome-wide identification and expression profiling of the bZIP gene family in Chrysanthemum indicum and the functional analysis of CibZIP29 under cadmium stress Ornamental Plant Research chrysanthemum indicum bzips cd stress cibzip29 |
| title | Genome-wide identification and expression profiling of the bZIP gene family in Chrysanthemum indicum and the functional analysis of CibZIP29 under cadmium stress |
| title_full | Genome-wide identification and expression profiling of the bZIP gene family in Chrysanthemum indicum and the functional analysis of CibZIP29 under cadmium stress |
| title_fullStr | Genome-wide identification and expression profiling of the bZIP gene family in Chrysanthemum indicum and the functional analysis of CibZIP29 under cadmium stress |
| title_full_unstemmed | Genome-wide identification and expression profiling of the bZIP gene family in Chrysanthemum indicum and the functional analysis of CibZIP29 under cadmium stress |
| title_short | Genome-wide identification and expression profiling of the bZIP gene family in Chrysanthemum indicum and the functional analysis of CibZIP29 under cadmium stress |
| title_sort | genome wide identification and expression profiling of the bzip gene family in chrysanthemum indicum and the functional analysis of cibzip29 under cadmium stress |
| topic | chrysanthemum indicum bzips cd stress cibzip29 |
| url | https://www.maxapress.com/article/doi/10.48130/opr-0025-0010 |
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