Silencing LINC00663 inhibits inflammation and angiogenesis through downregulation of NR2F1 via EBF1 in bladder cancer
This study is to elucidate the effect of the LINC00663/EBF1/NR2F1 axis on inflammation and angiogenesis in bladder cancer (BC) and related molecular mechanisms. After transfection, functional experiments were conducted to test cell proliferation and invasion, tube formation ability, and content of i...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2024-12-01
|
| Series: | RNA Biology |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/15476286.2024.2368304 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850263474426347520 |
|---|---|
| author | Xiulong Zhong Lijiang Sun Junxiang Liu Xiaokun Yang Minghui Hou Xinning Wang Huifeng Diao |
| author_facet | Xiulong Zhong Lijiang Sun Junxiang Liu Xiaokun Yang Minghui Hou Xinning Wang Huifeng Diao |
| author_sort | Xiulong Zhong |
| collection | DOAJ |
| description | This study is to elucidate the effect of the LINC00663/EBF1/NR2F1 axis on inflammation and angiogenesis in bladder cancer (BC) and related molecular mechanisms. After transfection, functional experiments were conducted to test cell proliferation and invasion, tube formation ability, and content of inflammatory factors, Snail, E-cadherin, and VEGFA. Meanwhile, the relationships among LINC00663, EBF1, and NR2F1 were predicted and verified. In addition, xenograft experiments in nude mice were performed to observe the oncogenicity of 5637 BC cells in vivo. In BC tissues and cells, LINC00663 and NR2F1 were upregulated. Silencing NR2F1 or LINC00663 repressed cell proliferation and invasion, weakened vascular mimicry in vitro, decreased inflammatory factor, Snail, and VEGFA levels, and increased expression of E-cadherin. LINC00663 positively regulated NR2F1 expression through EBF1. Additionally, in vivo experiments showed that NR2F1 upregulation reversed the suppression effects of LINC00663 silencing on tumour growth, inflammation, and angiogenesis. Silencing LINC00663 decreased NR2F1 expression by mediating EBF1, thereby inhibiting BC inflammation and angiogenesis. |
| format | Article |
| id | doaj-art-ea161a9f31ee454aad8d52b5d91e39ea |
| institution | OA Journals |
| issn | 1547-6286 1555-8584 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | RNA Biology |
| spelling | doaj-art-ea161a9f31ee454aad8d52b5d91e39ea2025-08-20T01:54:57ZengTaylor & Francis GroupRNA Biology1547-62861555-85842024-12-0121167368610.1080/15476286.2024.2368304Silencing LINC00663 inhibits inflammation and angiogenesis through downregulation of NR2F1 via EBF1 in bladder cancerXiulong Zhong0Lijiang Sun1Junxiang Liu2Xiaokun Yang3Minghui Hou4Xinning Wang5Huifeng Diao6Department of Urology Surgery, Affiliated Hospital of Qingdao University, Qingdao, Shandong, P.R. ChinaDepartment of Urology Surgery, Affiliated Hospital of Qingdao University, Qingdao, Shandong, P.R. ChinaDepartment of Urology Surgery, Affiliated Hospital of Qingdao University, Qingdao, Shandong, P.R. ChinaDepartment of Urology Surgery, Affiliated Hospital of Qingdao University, Qingdao, Shandong, P.R. ChinaDepartment of Urology Surgery, Affiliated Hospital of Qingdao University, Qingdao, Shandong, P.R. ChinaMedical Record Management Center, Affiliated Hospital of Qingdao University, Qingdao, Shandong, P.R. ChinaDepartment of Urology Surgery, Affiliated Hospital of Qingdao University, Qingdao, Shandong, P.R. ChinaThis study is to elucidate the effect of the LINC00663/EBF1/NR2F1 axis on inflammation and angiogenesis in bladder cancer (BC) and related molecular mechanisms. After transfection, functional experiments were conducted to test cell proliferation and invasion, tube formation ability, and content of inflammatory factors, Snail, E-cadherin, and VEGFA. Meanwhile, the relationships among LINC00663, EBF1, and NR2F1 were predicted and verified. In addition, xenograft experiments in nude mice were performed to observe the oncogenicity of 5637 BC cells in vivo. In BC tissues and cells, LINC00663 and NR2F1 were upregulated. Silencing NR2F1 or LINC00663 repressed cell proliferation and invasion, weakened vascular mimicry in vitro, decreased inflammatory factor, Snail, and VEGFA levels, and increased expression of E-cadherin. LINC00663 positively regulated NR2F1 expression through EBF1. Additionally, in vivo experiments showed that NR2F1 upregulation reversed the suppression effects of LINC00663 silencing on tumour growth, inflammation, and angiogenesis. Silencing LINC00663 decreased NR2F1 expression by mediating EBF1, thereby inhibiting BC inflammation and angiogenesis.https://www.tandfonline.com/doi/10.1080/15476286.2024.2368304LINC00663EBF1nuclear receptor subfamily 2 group F member 1bladder cancerinflammationangiogenesis |
| spellingShingle | Xiulong Zhong Lijiang Sun Junxiang Liu Xiaokun Yang Minghui Hou Xinning Wang Huifeng Diao Silencing LINC00663 inhibits inflammation and angiogenesis through downregulation of NR2F1 via EBF1 in bladder cancer RNA Biology LINC00663 EBF1 nuclear receptor subfamily 2 group F member 1 bladder cancer inflammation angiogenesis |
| title | Silencing LINC00663 inhibits inflammation and angiogenesis through downregulation of NR2F1 via EBF1 in bladder cancer |
| title_full | Silencing LINC00663 inhibits inflammation and angiogenesis through downregulation of NR2F1 via EBF1 in bladder cancer |
| title_fullStr | Silencing LINC00663 inhibits inflammation and angiogenesis through downregulation of NR2F1 via EBF1 in bladder cancer |
| title_full_unstemmed | Silencing LINC00663 inhibits inflammation and angiogenesis through downregulation of NR2F1 via EBF1 in bladder cancer |
| title_short | Silencing LINC00663 inhibits inflammation and angiogenesis through downregulation of NR2F1 via EBF1 in bladder cancer |
| title_sort | silencing linc00663 inhibits inflammation and angiogenesis through downregulation of nr2f1 via ebf1 in bladder cancer |
| topic | LINC00663 EBF1 nuclear receptor subfamily 2 group F member 1 bladder cancer inflammation angiogenesis |
| url | https://www.tandfonline.com/doi/10.1080/15476286.2024.2368304 |
| work_keys_str_mv | AT xiulongzhong silencinglinc00663inhibitsinflammationandangiogenesisthroughdownregulationofnr2f1viaebf1inbladdercancer AT lijiangsun silencinglinc00663inhibitsinflammationandangiogenesisthroughdownregulationofnr2f1viaebf1inbladdercancer AT junxiangliu silencinglinc00663inhibitsinflammationandangiogenesisthroughdownregulationofnr2f1viaebf1inbladdercancer AT xiaokunyang silencinglinc00663inhibitsinflammationandangiogenesisthroughdownregulationofnr2f1viaebf1inbladdercancer AT minghuihou silencinglinc00663inhibitsinflammationandangiogenesisthroughdownregulationofnr2f1viaebf1inbladdercancer AT xinningwang silencinglinc00663inhibitsinflammationandangiogenesisthroughdownregulationofnr2f1viaebf1inbladdercancer AT huifengdiao silencinglinc00663inhibitsinflammationandangiogenesisthroughdownregulationofnr2f1viaebf1inbladdercancer |