Molecular epidemiology and microbiological characteristics of Cryptococcus gattii VGII isolates from China.
Cryptococcus gattii (C. gattii) is a fungal pathogen that once caused an outbreak of cryptococcosis on Vancouver Island, and had spread worldwide, while few data were available in China. In this study, seven clinical isolates of C. gattii VGII were collected from 19 hospitals, Multi-locus Sequence T...
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Public Library of Science (PLoS)
2022-02-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0010078&type=printable |
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| author | Xuelei Zang Weixin Ke Lifeng Wang Hua Wu Yemei Huang Hengyu Deng Meng Zhou Ningxin Wu Xinying Xue Dingxia Shen |
| author_facet | Xuelei Zang Weixin Ke Lifeng Wang Hua Wu Yemei Huang Hengyu Deng Meng Zhou Ningxin Wu Xinying Xue Dingxia Shen |
| author_sort | Xuelei Zang |
| collection | DOAJ |
| description | Cryptococcus gattii (C. gattii) is a fungal pathogen that once caused an outbreak of cryptococcosis on Vancouver Island, and had spread worldwide, while few data were available in China. In this study, seven clinical isolates of C. gattii VGII were collected from 19 hospitals, Multi-locus Sequence Typing (MLST) analysis and whole-genome sequencing (WGS) was performed, combined with published data for phylogenetic analysis. In addition, in vitro antifungal susceptibility testing, phenotypic analysis, and in vivo virulence studies were performed, subsequently, histopathological analysis of lung tissue was performed. C.gattii VGII infected patients were mainly immunocompetent male, and most of them had symptoms of central nervous system (CNS) involvement. MLST results showed that isolates from China exhibited high genetic diversity, and sequence type (ST) 7 was the major ST among the isolates. Some clinical isolates showed a close phylogenetic relationship with strains from Australia and South America. All clinical isolates did not show resistance to antifungal drugs. In addition, there was no correlation between virulence factors (temperature, melanin production, and capsule size) and virulence while in vivo experiments showed significant differences in virulence among strains. Lung fungal burden and damage to lung tissue correlated with virulence, and degree of damage to lung tissue in mice may highlight differences in virulence. Our work seeks to provide useful data for molecular epidemiology, antifungal susceptibility, and virulence differences of C. gattii VGII in China. |
| format | Article |
| id | doaj-art-ea160be4ea48487bbb53ca3454c3b7db |
| institution | DOAJ |
| issn | 1935-2727 1935-2735 |
| language | English |
| publishDate | 2022-02-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj-art-ea160be4ea48487bbb53ca3454c3b7db2025-08-20T03:00:05ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352022-02-01162e001007810.1371/journal.pntd.0010078Molecular epidemiology and microbiological characteristics of Cryptococcus gattii VGII isolates from China.Xuelei ZangWeixin KeLifeng WangHua WuYemei HuangHengyu DengMeng ZhouNingxin WuXinying XueDingxia ShenCryptococcus gattii (C. gattii) is a fungal pathogen that once caused an outbreak of cryptococcosis on Vancouver Island, and had spread worldwide, while few data were available in China. In this study, seven clinical isolates of C. gattii VGII were collected from 19 hospitals, Multi-locus Sequence Typing (MLST) analysis and whole-genome sequencing (WGS) was performed, combined with published data for phylogenetic analysis. In addition, in vitro antifungal susceptibility testing, phenotypic analysis, and in vivo virulence studies were performed, subsequently, histopathological analysis of lung tissue was performed. C.gattii VGII infected patients were mainly immunocompetent male, and most of them had symptoms of central nervous system (CNS) involvement. MLST results showed that isolates from China exhibited high genetic diversity, and sequence type (ST) 7 was the major ST among the isolates. Some clinical isolates showed a close phylogenetic relationship with strains from Australia and South America. All clinical isolates did not show resistance to antifungal drugs. In addition, there was no correlation between virulence factors (temperature, melanin production, and capsule size) and virulence while in vivo experiments showed significant differences in virulence among strains. Lung fungal burden and damage to lung tissue correlated with virulence, and degree of damage to lung tissue in mice may highlight differences in virulence. Our work seeks to provide useful data for molecular epidemiology, antifungal susceptibility, and virulence differences of C. gattii VGII in China.https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0010078&type=printable |
| spellingShingle | Xuelei Zang Weixin Ke Lifeng Wang Hua Wu Yemei Huang Hengyu Deng Meng Zhou Ningxin Wu Xinying Xue Dingxia Shen Molecular epidemiology and microbiological characteristics of Cryptococcus gattii VGII isolates from China. PLoS Neglected Tropical Diseases |
| title | Molecular epidemiology and microbiological characteristics of Cryptococcus gattii VGII isolates from China. |
| title_full | Molecular epidemiology and microbiological characteristics of Cryptococcus gattii VGII isolates from China. |
| title_fullStr | Molecular epidemiology and microbiological characteristics of Cryptococcus gattii VGII isolates from China. |
| title_full_unstemmed | Molecular epidemiology and microbiological characteristics of Cryptococcus gattii VGII isolates from China. |
| title_short | Molecular epidemiology and microbiological characteristics of Cryptococcus gattii VGII isolates from China. |
| title_sort | molecular epidemiology and microbiological characteristics of cryptococcus gattii vgii isolates from china |
| url | https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0010078&type=printable |
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