Effects of fermented Broussonetia papyrifera on the laying performance, egg quality, and gut microbiota of Taihe silk chicken during the peak laying period

Taihe silk chicken (Gallus gallus domesticus Brisson) are prized for their nutritional value but face challenges like low productivity and feed efficiency. Broussonetia papyrifera (BP), rich in nutrients, is mainly used in ruminant feed. This study investigates the effects of fermented BP (FBP) on t...

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Main Authors: Yang Fu, Lutong Zhou, Yutian Shen, Weifa Su, Wentao Li, Lixia Kai, Wei Wei, Yuanzhi Cheng, Fengqin Wang, Yizhen Wang, Jianjun Peng, Zeqing Lu
Format: Article
Language:English
Published: Cambridge University Press 2025-01-01
Series:Animal Nutriomics
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Online Access:https://www.cambridge.org/core/product/identifier/S2977377625000079/type/journal_article
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author Yang Fu
Lutong Zhou
Yutian Shen
Weifa Su
Wentao Li
Lixia Kai
Wei Wei
Yuanzhi Cheng
Fengqin Wang
Yizhen Wang
Jianjun Peng
Zeqing Lu
author_facet Yang Fu
Lutong Zhou
Yutian Shen
Weifa Su
Wentao Li
Lixia Kai
Wei Wei
Yuanzhi Cheng
Fengqin Wang
Yizhen Wang
Jianjun Peng
Zeqing Lu
author_sort Yang Fu
collection DOAJ
description Taihe silk chicken (Gallus gallus domesticus Brisson) are prized for their nutritional value but face challenges like low productivity and feed efficiency. Broussonetia papyrifera (BP), rich in nutrients, is mainly used in ruminant feed. This study investigates the effects of fermented BP (FBP) on the laying performance, egg quality, and gut microbiota of Taihe silk chicken during peak laying period. A total of 240 chickens were randomly assigned to four treatments (five replicates/treatments) with a basal diet (CON), a basal diet + 2% FBP (T2), a basal diet + 4% FBP (T4), and a basal diet + 8% FBP (T8) for 75 d. Results showed that the average daily feed intake and yolk color in the 8% FBP group were significantly increased by 12.21% and 11.78%, respectively (P < 0.05). Yolk folate content of the 4% and 8% FBP groups was significantly increased by 32.73% and 59.76%, respectively (P < 0.05). Zinc content in the yolk of the 8% FBP group was significantly increased by 14.22% (P< 0.05). The FBP group influenced the fatty acid composition of the yolk, and 8% FBP significantly decreased the n-6 unsaturated fatty acid (PUFA) to n-3 PUFA ratio (P< 0.05). FBP also increased the ratio of villus height, and crypt depth significantly increased in the duodenum, jejunum and ileum (P< 0.05). The 16S rRNA sequencing revealed that FBP altered cecal microbiota, increasing the relative abundance of Bacteroides, Rikenellaceae_RC9_gut_group, and Alistipes, while reducing the relative abundance of Olsenella and Ruminococcaceae UCG-005. Correlation analysis suggests that the FBP may enhance the growth performance and egg composition by modulating gut microbiota. In conclusion, this study confirms that adding FBP to the diet improves egg quality, composition, intestinal structure, and gut microbiota in Taihe silk chicken. These insights are valuable for optimizing FBP utilization in Taihe silk chicken production.
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spelling doaj-art-ea0c28eb0f5941d78aca476437abec8e2025-08-22T11:13:33ZengCambridge University PressAnimal Nutriomics2977-37762977-37842025-01-01210.1017/anr.2025.7Effects of fermented Broussonetia papyrifera on the laying performance, egg quality, and gut microbiota of Taihe silk chicken during the peak laying periodYang Fu0https://orcid.org/0009-0007-8557-938XLutong Zhou1Yutian Shen2Weifa Su3Wentao Li4Lixia Kai5Wei Wei6Yuanzhi Cheng7Fengqin Wang8Yizhen Wang9Jianjun Peng10Zeqing Lu11https://orcid.org/0000-0002-0720-4693National Engineering Research Centre for Green Feed and Healthy Farming, Zhejiang University, Hangzhou, China Key Laboratory of Molecular Nutrition, Ministry of Education, Zhejiang University, Hangzhou, China Key Laboratory of Animal Nutrition and Feed, Ministry of Agriculture and Rural Affairs, Zhejiang University, Hangzhou, China Zhejiang Key Laboratory of Nutrition and Breeding for High-Quality Animal Products, Zhejiang University, Hangzhou, ChinaNational Engineering Research Centre for Green Feed and Healthy Farming, Zhejiang University, Hangzhou, China Key Laboratory of Molecular Nutrition, Ministry of Education, Zhejiang University, Hangzhou, China Key Laboratory of Animal Nutrition and Feed, Ministry of Agriculture and Rural Affairs, Zhejiang University, Hangzhou, China Zhejiang Key Laboratory of Nutrition and Breeding for High-Quality Animal Products, Zhejiang University, Hangzhou, ChinaNational Engineering Research Centre for Green Feed and Healthy Farming, Zhejiang University, Hangzhou, China Key Laboratory of Molecular Nutrition, Ministry of Education, Zhejiang University, Hangzhou, China Key Laboratory of Animal Nutrition and Feed, Ministry of Agriculture and Rural Affairs, Zhejiang University, Hangzhou, China Zhejiang Key Laboratory of Nutrition and Breeding for High-Quality Animal Products, Zhejiang University, Hangzhou, ChinaNational Engineering Research Centre for Green Feed and Healthy Farming, Zhejiang University, Hangzhou, China Key Laboratory of Molecular Nutrition, Ministry of Education, Zhejiang University, Hangzhou, China Key Laboratory of Animal Nutrition and Feed, Ministry of Agriculture and Rural Affairs, Zhejiang University, Hangzhou, China Zhejiang Key Laboratory of Nutrition and Breeding for High-Quality Animal Products, Zhejiang University, Hangzhou, ChinaNational Engineering Research Centre for Green Feed and Healthy Farming, Zhejiang University, Hangzhou, China Key Laboratory of Molecular Nutrition, Ministry of Education, Zhejiang University, Hangzhou, China Key Laboratory of Animal Nutrition and Feed, Ministry of Agriculture and Rural Affairs, Zhejiang University, Hangzhou, China Zhejiang Key Laboratory of Nutrition and Breeding for High-Quality Animal Products, Zhejiang University, Hangzhou, ChinaNational Engineering Research Centre for Green Feed and Healthy Farming, Zhejiang University, Hangzhou, China Key Laboratory of Molecular Nutrition, Ministry of Education, Zhejiang University, Hangzhou, China Key Laboratory of Animal Nutrition and Feed, Ministry of Agriculture and Rural Affairs, Zhejiang University, Hangzhou, China Zhejiang Key Laboratory of Nutrition and Breeding for High-Quality Animal Products, Zhejiang University, Hangzhou, ChinaNational Engineering Research Centre for Green Feed and Healthy Farming, Zhejiang University, Hangzhou, China Key Laboratory of Molecular Nutrition, Ministry of Education, Zhejiang University, Hangzhou, China Key Laboratory of Animal Nutrition and Feed, Ministry of Agriculture and Rural Affairs, Zhejiang University, Hangzhou, China Zhejiang Key Laboratory of Nutrition and Breeding for High-Quality Animal Products, Zhejiang University, Hangzhou, ChinaNational Engineering Research Centre for Green Feed and Healthy Farming, Zhejiang University, Hangzhou, China Key Laboratory of Molecular Nutrition, Ministry of Education, Zhejiang University, Hangzhou, China Key Laboratory of Animal Nutrition and Feed, Ministry of Agriculture and Rural Affairs, Zhejiang University, Hangzhou, China Zhejiang Key Laboratory of Nutrition and Breeding for High-Quality Animal Products, Zhejiang University, Hangzhou, ChinaNational Engineering Research Centre for Green Feed and Healthy Farming, Zhejiang University, Hangzhou, China Key Laboratory of Molecular Nutrition, Ministry of Education, Zhejiang University, Hangzhou, China Key Laboratory of Animal Nutrition and Feed, Ministry of Agriculture and Rural Affairs, Zhejiang University, Hangzhou, China Zhejiang Key Laboratory of Nutrition and Breeding for High-Quality Animal Products, Zhejiang University, Hangzhou, ChinaNational Engineering Research Centre for Green Feed and Healthy Farming, Zhejiang University, Hangzhou, China Key Laboratory of Molecular Nutrition, Ministry of Education, Zhejiang University, Hangzhou, China Key Laboratory of Animal Nutrition and Feed, Ministry of Agriculture and Rural Affairs, Zhejiang University, Hangzhou, China Zhejiang Key Laboratory of Nutrition and Breeding for High-Quality Animal Products, Zhejiang University, Hangzhou, ChinaXichang Fengxiang Poultry Industry Co., Ltd, Taihe County, Ji’an, ChinaNational Engineering Research Centre for Green Feed and Healthy Farming, Zhejiang University, Hangzhou, China Key Laboratory of Molecular Nutrition, Ministry of Education, Zhejiang University, Hangzhou, China Key Laboratory of Animal Nutrition and Feed, Ministry of Agriculture and Rural Affairs, Zhejiang University, Hangzhou, China Zhejiang Key Laboratory of Nutrition and Breeding for High-Quality Animal Products, Zhejiang University, Hangzhou, ChinaTaihe silk chicken (Gallus gallus domesticus Brisson) are prized for their nutritional value but face challenges like low productivity and feed efficiency. Broussonetia papyrifera (BP), rich in nutrients, is mainly used in ruminant feed. This study investigates the effects of fermented BP (FBP) on the laying performance, egg quality, and gut microbiota of Taihe silk chicken during peak laying period. A total of 240 chickens were randomly assigned to four treatments (five replicates/treatments) with a basal diet (CON), a basal diet + 2% FBP (T2), a basal diet + 4% FBP (T4), and a basal diet + 8% FBP (T8) for 75 d. Results showed that the average daily feed intake and yolk color in the 8% FBP group were significantly increased by 12.21% and 11.78%, respectively (P < 0.05). Yolk folate content of the 4% and 8% FBP groups was significantly increased by 32.73% and 59.76%, respectively (P < 0.05). Zinc content in the yolk of the 8% FBP group was significantly increased by 14.22% (P< 0.05). The FBP group influenced the fatty acid composition of the yolk, and 8% FBP significantly decreased the n-6 unsaturated fatty acid (PUFA) to n-3 PUFA ratio (P< 0.05). FBP also increased the ratio of villus height, and crypt depth significantly increased in the duodenum, jejunum and ileum (P< 0.05). The 16S rRNA sequencing revealed that FBP altered cecal microbiota, increasing the relative abundance of Bacteroides, Rikenellaceae_RC9_gut_group, and Alistipes, while reducing the relative abundance of Olsenella and Ruminococcaceae UCG-005. Correlation analysis suggests that the FBP may enhance the growth performance and egg composition by modulating gut microbiota. In conclusion, this study confirms that adding FBP to the diet improves egg quality, composition, intestinal structure, and gut microbiota in Taihe silk chicken. These insights are valuable for optimizing FBP utilization in Taihe silk chicken production.https://www.cambridge.org/core/product/identifier/S2977377625000079/type/journal_articlefermented Broussonetia papyriferaTaihe silk chickenlaying performanceegg qualitygut microbiota
spellingShingle Yang Fu
Lutong Zhou
Yutian Shen
Weifa Su
Wentao Li
Lixia Kai
Wei Wei
Yuanzhi Cheng
Fengqin Wang
Yizhen Wang
Jianjun Peng
Zeqing Lu
Effects of fermented Broussonetia papyrifera on the laying performance, egg quality, and gut microbiota of Taihe silk chicken during the peak laying period
Animal Nutriomics
fermented Broussonetia papyrifera
Taihe silk chicken
laying performance
egg quality
gut microbiota
title Effects of fermented Broussonetia papyrifera on the laying performance, egg quality, and gut microbiota of Taihe silk chicken during the peak laying period
title_full Effects of fermented Broussonetia papyrifera on the laying performance, egg quality, and gut microbiota of Taihe silk chicken during the peak laying period
title_fullStr Effects of fermented Broussonetia papyrifera on the laying performance, egg quality, and gut microbiota of Taihe silk chicken during the peak laying period
title_full_unstemmed Effects of fermented Broussonetia papyrifera on the laying performance, egg quality, and gut microbiota of Taihe silk chicken during the peak laying period
title_short Effects of fermented Broussonetia papyrifera on the laying performance, egg quality, and gut microbiota of Taihe silk chicken during the peak laying period
title_sort effects of fermented broussonetia papyrifera on the laying performance egg quality and gut microbiota of taihe silk chicken during the peak laying period
topic fermented Broussonetia papyrifera
Taihe silk chicken
laying performance
egg quality
gut microbiota
url https://www.cambridge.org/core/product/identifier/S2977377625000079/type/journal_article
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