EN 1785:2003 method for the identification of ionizing treatment in poultry meat products: optimization of the accelerated solvent extraction procedure

X-ray irradiation is a non-thermal technology used for food preservation and sanitization to prolong shelf life. To evaluate the fraudulent use of X-ray treatment, in this study, an accelerated solvent extraction method was optimized for the extraction of lipids and 2-dodecylcyclobutanone (2-DCB),...

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Main Authors: Maria Campaniello, Rosalia Zianni, Andrea Chiappinelli, Annalisa Mentana, Valeria Nardelli
Format: Article
Language:English
Published: PAGEPress Publications 2025-06-01
Series:Italian Journal of Food Safety
Subjects:
Online Access:https://www.pagepressjournals.org/ijfs/article/view/13501
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author Maria Campaniello
Rosalia Zianni
Andrea Chiappinelli
Annalisa Mentana
Valeria Nardelli
author_facet Maria Campaniello
Rosalia Zianni
Andrea Chiappinelli
Annalisa Mentana
Valeria Nardelli
author_sort Maria Campaniello
collection DOAJ
description X-ray irradiation is a non-thermal technology used for food preservation and sanitization to prolong shelf life. To evaluate the fraudulent use of X-ray treatment, in this study, an accelerated solvent extraction method was optimized for the extraction of lipids and 2-dodecylcyclobutanone (2-DCB), reducing analysis time and solvent consumption compared to Soxhlet extraction reported in the standard EN 1785:2003 method. The qualitative confirmation method was tested on different processed poultry meats, i.e., sausages and hamburgers, irradiated at different dose levels, that is, 0.5, 1.0, and 3.0 kGy. The analytical parameters investigated, namely method linearity, selectivity, minimum dose level, diagnostic sensitivity and specificity, and recovery, showed that the proposed method is suitable for routine analysis in official controls to determine 2-DCB as an irradiation marker.
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institution Kabale University
issn 2239-7132
language English
publishDate 2025-06-01
publisher PAGEPress Publications
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series Italian Journal of Food Safety
spelling doaj-art-ea05db27e97046978a31a41c1e686b0a2025-08-20T03:27:09ZengPAGEPress PublicationsItalian Journal of Food Safety2239-71322025-06-0110.4081/ijfs.2025.13501EN 1785:2003 method for the identification of ionizing treatment in poultry meat products: optimization of the accelerated solvent extraction procedureMaria Campaniello0Rosalia Zianni1Andrea Chiappinelli2Annalisa Mentana3Valeria Nardelli4National Reference Laboratory for the treatment of foods and their ingredients with ionizing radiation, Chemistry Department, Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, FoggiaNational Reference Laboratory for the treatment of foods and their ingredients with ionizing radiation, Chemistry Department, Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, FoggiaNational Reference Laboratory for the treatment of foods and their ingredients with ionizing radiation, Chemistry Department, Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, FoggiaNational Reference Laboratory for the treatment of foods and their ingredients with ionizing radiation, Chemistry Department, Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, FoggiaNational Reference Laboratory for the treatment of foods and their ingredients with ionizing radiation, Chemistry Department, Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Foggia X-ray irradiation is a non-thermal technology used for food preservation and sanitization to prolong shelf life. To evaluate the fraudulent use of X-ray treatment, in this study, an accelerated solvent extraction method was optimized for the extraction of lipids and 2-dodecylcyclobutanone (2-DCB), reducing analysis time and solvent consumption compared to Soxhlet extraction reported in the standard EN 1785:2003 method. The qualitative confirmation method was tested on different processed poultry meats, i.e., sausages and hamburgers, irradiated at different dose levels, that is, 0.5, 1.0, and 3.0 kGy. The analytical parameters investigated, namely method linearity, selectivity, minimum dose level, diagnostic sensitivity and specificity, and recovery, showed that the proposed method is suitable for routine analysis in official controls to determine 2-DCB as an irradiation marker. https://www.pagepressjournals.org/ijfs/article/view/13501Food irradiationfood preservationmeat2-DCBaccelerated solvent extraction
spellingShingle Maria Campaniello
Rosalia Zianni
Andrea Chiappinelli
Annalisa Mentana
Valeria Nardelli
EN 1785:2003 method for the identification of ionizing treatment in poultry meat products: optimization of the accelerated solvent extraction procedure
Italian Journal of Food Safety
Food irradiation
food preservation
meat
2-DCB
accelerated solvent extraction
title EN 1785:2003 method for the identification of ionizing treatment in poultry meat products: optimization of the accelerated solvent extraction procedure
title_full EN 1785:2003 method for the identification of ionizing treatment in poultry meat products: optimization of the accelerated solvent extraction procedure
title_fullStr EN 1785:2003 method for the identification of ionizing treatment in poultry meat products: optimization of the accelerated solvent extraction procedure
title_full_unstemmed EN 1785:2003 method for the identification of ionizing treatment in poultry meat products: optimization of the accelerated solvent extraction procedure
title_short EN 1785:2003 method for the identification of ionizing treatment in poultry meat products: optimization of the accelerated solvent extraction procedure
title_sort en 1785 2003 method for the identification of ionizing treatment in poultry meat products optimization of the accelerated solvent extraction procedure
topic Food irradiation
food preservation
meat
2-DCB
accelerated solvent extraction
url https://www.pagepressjournals.org/ijfs/article/view/13501
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