Serological, cultural, and molecular evidence of Brucella infection in small ruminants in Pakistan
Introduction: The objectives of the present study were to determine the seroprevalence and identify the causative agent of brucellosis in small ruminants in Pakistan. Methodology: A total of 278 serum and 212 milk samples were collected from sheep and goats that had close contact with seropositive...
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The Journal of Infection in Developing Countries
2015-05-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/5110 |
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| author | Shahzad Ali Shamim Akhter Heinrich Neubauer Falk Melzer Iahtasham Khan Qurban Ali Muhammad Irfan |
| author_facet | Shahzad Ali Shamim Akhter Heinrich Neubauer Falk Melzer Iahtasham Khan Qurban Ali Muhammad Irfan |
| author_sort | Shahzad Ali |
| collection | DOAJ |
| description | Introduction: The objectives of the present study were to determine the seroprevalence and identify the causative agent of brucellosis in small ruminants in Pakistan.
Methodology: A total of 278 serum and 212 milk samples were collected from sheep and goats that had close contact with seropositive bovine herds. Data related to age, sex, location, and breed were collected on the sampling day. Serum and milk samples were initially screened using two different Rose Bengal plate test (RBPT) antigens and a milk ring test (MRT). Seropositive samples were subjected to bacterial isolation and PCR analysis using Brucella genus-specific (bcsp31) and Brucella species-specific (IS711 for Brucella abortus and Brucella melitensis) quantitative real-time polymerase chain reactions (qRT-PCR).
Results: Twenty-four (8.6%) serum samples were positive by RBPT. Twenty (9.4%) animals were positive for Brucella antibodies using MRT. No Brucella isolates were obtained from the examined blood and milk samples. Of the 24 seropositive serum samples, 18 (75%) were positive in the Brucella genus-specific (bcsp31) and Brucella abortus-specific (IS711) qRT-PCR, respectively.
Conclusions: Brucella abortus was identified as causative agent of ovine and caprine brucellosis in Pakistan. Results of this study can be used for the development of an effective control and eradication strategy for brucellosis in livestock, especially small ruminants.
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| format | Article |
| id | doaj-art-ea01f04ec4eb4985971062c9877d2838 |
| institution | DOAJ |
| issn | 1972-2680 |
| language | English |
| publishDate | 2015-05-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-ea01f04ec4eb4985971062c9877d28382025-08-20T02:57:12ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802015-05-0190510.3855/jidc.5110Serological, cultural, and molecular evidence of Brucella infection in small ruminants in PakistanShahzad Ali0Shamim Akhter1Heinrich Neubauer2Falk Melzer3Iahtasham Khan4Qurban Ali5Muhammad Irfan6University of Veterinary and Animal Sciences, Lahore, PakistanPir Mehr Ali Shah Arid Agriculture University Rawalpindi, PakistanFriedrich-Loeffler-Institut, Jena, GermanyFriedrich-Loeffler-Institut, Jena, GermanyFriedrich-Loeffler-Institut, Jena, GermanyNational Veterinary Laboratory, Park Road, Islamabad, PakistanNational Veterinary Laboratory, Park Road, Islamabad, PakistanIntroduction: The objectives of the present study were to determine the seroprevalence and identify the causative agent of brucellosis in small ruminants in Pakistan. Methodology: A total of 278 serum and 212 milk samples were collected from sheep and goats that had close contact with seropositive bovine herds. Data related to age, sex, location, and breed were collected on the sampling day. Serum and milk samples were initially screened using two different Rose Bengal plate test (RBPT) antigens and a milk ring test (MRT). Seropositive samples were subjected to bacterial isolation and PCR analysis using Brucella genus-specific (bcsp31) and Brucella species-specific (IS711 for Brucella abortus and Brucella melitensis) quantitative real-time polymerase chain reactions (qRT-PCR). Results: Twenty-four (8.6%) serum samples were positive by RBPT. Twenty (9.4%) animals were positive for Brucella antibodies using MRT. No Brucella isolates were obtained from the examined blood and milk samples. Of the 24 seropositive serum samples, 18 (75%) were positive in the Brucella genus-specific (bcsp31) and Brucella abortus-specific (IS711) qRT-PCR, respectively. Conclusions: Brucella abortus was identified as causative agent of ovine and caprine brucellosis in Pakistan. Results of this study can be used for the development of an effective control and eradication strategy for brucellosis in livestock, especially small ruminants. https://jidc.org/index.php/journal/article/view/5110brucellosissheepgoatseroprevalencePCRPakistan |
| spellingShingle | Shahzad Ali Shamim Akhter Heinrich Neubauer Falk Melzer Iahtasham Khan Qurban Ali Muhammad Irfan Serological, cultural, and molecular evidence of Brucella infection in small ruminants in Pakistan Journal of Infection in Developing Countries brucellosis sheep goat seroprevalence PCR Pakistan |
| title | Serological, cultural, and molecular evidence of Brucella infection in small ruminants in Pakistan |
| title_full | Serological, cultural, and molecular evidence of Brucella infection in small ruminants in Pakistan |
| title_fullStr | Serological, cultural, and molecular evidence of Brucella infection in small ruminants in Pakistan |
| title_full_unstemmed | Serological, cultural, and molecular evidence of Brucella infection in small ruminants in Pakistan |
| title_short | Serological, cultural, and molecular evidence of Brucella infection in small ruminants in Pakistan |
| title_sort | serological cultural and molecular evidence of brucella infection in small ruminants in pakistan |
| topic | brucellosis sheep goat seroprevalence PCR Pakistan |
| url | https://jidc.org/index.php/journal/article/view/5110 |
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