Comparative Proteomic Analysis of Paulownia fortunei Response to Phytoplasma Infection with Dimethyl Sulfate Treatment
Paulownia fortunei is a widely cultivated economic forest tree species that is susceptible to infection with phytoplasma, resulting in Paulownia witches’ broom (PaWB) disease. Diseased P. fortunei is characterized by stunted growth, witches’ broom, shortened internodes, and etiolated and smaller lea...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2017/6542075 |
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| author | Zhen Wei Zhe Wang Xiaoyu Li Zhenli Zhao Minjie Deng Yanpeng Dong Xibing Cao Guoqiang Fan |
| author_facet | Zhen Wei Zhe Wang Xiaoyu Li Zhenli Zhao Minjie Deng Yanpeng Dong Xibing Cao Guoqiang Fan |
| author_sort | Zhen Wei |
| collection | DOAJ |
| description | Paulownia fortunei is a widely cultivated economic forest tree species that is susceptible to infection with phytoplasma, resulting in Paulownia witches’ broom (PaWB) disease. Diseased P. fortunei is characterized by stunted growth, witches’ broom, shortened internodes, and etiolated and smaller leaves. To understand the molecular mechanism of its pathogenesis, we applied isobaric tags for relative and absolute quantitation (iTRAQ) and liquid chromatography coupled with tandem mass spectrometry approaches to study changes in the proteomes of healthy P. fortunei, PaWB-infected P. fortunei, and PaWB-infected P. fortunei treated with 15 mg·L−1 or 75 mg·L−1 dimethyl sulfate. We identified 2969 proteins and 104 and 32 differentially abundant proteins that were phytoplasma infection responsive and dimethyl sulfate responsive, respectively. Based on our analysis of the different proteomes, 27 PaWB-related proteins were identified. The protein-protein interactions of these 27 proteins were analyzed and classified into four groups (photosynthesis-related, energy-related, ribosome-related, and individual proteins). These PaWB-related proteins may help in developing a deeper understanding of how PaWB affects the morphological characteristics of P. fortunei and further establish the mechanisms involved in the response of P. fortunei to phytoplasma. |
| format | Article |
| id | doaj-art-e9f6506131194f5c9705a269f0dccb26 |
| institution | OA Journals |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-e9f6506131194f5c9705a269f0dccb262025-08-20T02:21:54ZengWileyInternational Journal of Genomics2314-436X2314-43782017-01-01201710.1155/2017/65420756542075Comparative Proteomic Analysis of Paulownia fortunei Response to Phytoplasma Infection with Dimethyl Sulfate TreatmentZhen Wei0Zhe Wang1Xiaoyu Li2Zhenli Zhao3Minjie Deng4Yanpeng Dong5Xibing Cao6Guoqiang Fan7Institute of Paulownia, Henan Agricultural University, Zhengzhou, Henan 450002, ChinaInstitute of Paulownia, Henan Agricultural University, Zhengzhou, Henan 450002, ChinaInstitute of Paulownia, Henan Agricultural University, Zhengzhou, Henan 450002, ChinaInstitute of Paulownia, Henan Agricultural University, Zhengzhou, Henan 450002, ChinaInstitute of Paulownia, Henan Agricultural University, Zhengzhou, Henan 450002, ChinaInstitute of Paulownia, Henan Agricultural University, Zhengzhou, Henan 450002, ChinaInstitute of Paulownia, Henan Agricultural University, Zhengzhou, Henan 450002, ChinaInstitute of Paulownia, Henan Agricultural University, Zhengzhou, Henan 450002, ChinaPaulownia fortunei is a widely cultivated economic forest tree species that is susceptible to infection with phytoplasma, resulting in Paulownia witches’ broom (PaWB) disease. Diseased P. fortunei is characterized by stunted growth, witches’ broom, shortened internodes, and etiolated and smaller leaves. To understand the molecular mechanism of its pathogenesis, we applied isobaric tags for relative and absolute quantitation (iTRAQ) and liquid chromatography coupled with tandem mass spectrometry approaches to study changes in the proteomes of healthy P. fortunei, PaWB-infected P. fortunei, and PaWB-infected P. fortunei treated with 15 mg·L−1 or 75 mg·L−1 dimethyl sulfate. We identified 2969 proteins and 104 and 32 differentially abundant proteins that were phytoplasma infection responsive and dimethyl sulfate responsive, respectively. Based on our analysis of the different proteomes, 27 PaWB-related proteins were identified. The protein-protein interactions of these 27 proteins were analyzed and classified into four groups (photosynthesis-related, energy-related, ribosome-related, and individual proteins). These PaWB-related proteins may help in developing a deeper understanding of how PaWB affects the morphological characteristics of P. fortunei and further establish the mechanisms involved in the response of P. fortunei to phytoplasma.http://dx.doi.org/10.1155/2017/6542075 |
| spellingShingle | Zhen Wei Zhe Wang Xiaoyu Li Zhenli Zhao Minjie Deng Yanpeng Dong Xibing Cao Guoqiang Fan Comparative Proteomic Analysis of Paulownia fortunei Response to Phytoplasma Infection with Dimethyl Sulfate Treatment International Journal of Genomics |
| title | Comparative Proteomic Analysis of Paulownia fortunei Response to Phytoplasma Infection with Dimethyl Sulfate Treatment |
| title_full | Comparative Proteomic Analysis of Paulownia fortunei Response to Phytoplasma Infection with Dimethyl Sulfate Treatment |
| title_fullStr | Comparative Proteomic Analysis of Paulownia fortunei Response to Phytoplasma Infection with Dimethyl Sulfate Treatment |
| title_full_unstemmed | Comparative Proteomic Analysis of Paulownia fortunei Response to Phytoplasma Infection with Dimethyl Sulfate Treatment |
| title_short | Comparative Proteomic Analysis of Paulownia fortunei Response to Phytoplasma Infection with Dimethyl Sulfate Treatment |
| title_sort | comparative proteomic analysis of paulownia fortunei response to phytoplasma infection with dimethyl sulfate treatment |
| url | http://dx.doi.org/10.1155/2017/6542075 |
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