Extraction conditions of polyphenol oxidase from banana peel
Polyphenol oxidase (PPO) is an enzyme containing copper, presents in various fruits and vegetables. It is responsible for the browning reactions when the cells are damaged during handling. The best conditions for extraction of polyphenol oxidase from banana peel was by using an extraction buffer con...
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University of Baghdad, College of Science for Women
2016-09-01
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| Series: | مجلة بغداد للعلوم |
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| Online Access: | http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/2292 |
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| author | Baghdad Science Journal |
| author_facet | Baghdad Science Journal |
| author_sort | Baghdad Science Journal |
| collection | DOAJ |
| description | Polyphenol oxidase (PPO) is an enzyme containing copper, presents in various fruits and vegetables. It is responsible for the browning reactions when the cells are damaged during handling. The best conditions for extraction of polyphenol oxidase from banana peel was by using an extraction buffer containing phosphate buffer (0.05 M, pH 7), 0.01 M ascorbic acid and 0.5% polyethylene glycol, with extraction ratio 1:4 (w:v) for one minute by using blender. The enzyme activity was measured spectrophotometrically at 425 nm. PPO was studied to prevent the browning of banana peel which results in the loss of their marketability. The aim of this study was to determine the optimum conditions for polyphenol oxidase extraction from banana peel. |
| format | Article |
| id | doaj-art-e9f5b47a3c9941d59108188044a3c1a5 |
| institution | DOAJ |
| issn | 2078-8665 2411-7986 |
| language | English |
| publishDate | 2016-09-01 |
| publisher | University of Baghdad, College of Science for Women |
| record_format | Article |
| series | مجلة بغداد للعلوم |
| spelling | doaj-art-e9f5b47a3c9941d59108188044a3c1a52025-08-20T02:50:59ZengUniversity of Baghdad, College of Science for Womenمجلة بغداد للعلوم2078-86652411-79862016-09-0113310.21123/bsj.13.3.469-474Extraction conditions of polyphenol oxidase from banana peelBaghdad Science JournalPolyphenol oxidase (PPO) is an enzyme containing copper, presents in various fruits and vegetables. It is responsible for the browning reactions when the cells are damaged during handling. The best conditions for extraction of polyphenol oxidase from banana peel was by using an extraction buffer containing phosphate buffer (0.05 M, pH 7), 0.01 M ascorbic acid and 0.5% polyethylene glycol, with extraction ratio 1:4 (w:v) for one minute by using blender. The enzyme activity was measured spectrophotometrically at 425 nm. PPO was studied to prevent the browning of banana peel which results in the loss of their marketability. The aim of this study was to determine the optimum conditions for polyphenol oxidase extraction from banana peel.http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/2292"Polyphenol oxidase; Banana peel; Extraction" |
| spellingShingle | Baghdad Science Journal Extraction conditions of polyphenol oxidase from banana peel مجلة بغداد للعلوم "Polyphenol oxidase; Banana peel; Extraction" |
| title | Extraction conditions of polyphenol oxidase from banana peel |
| title_full | Extraction conditions of polyphenol oxidase from banana peel |
| title_fullStr | Extraction conditions of polyphenol oxidase from banana peel |
| title_full_unstemmed | Extraction conditions of polyphenol oxidase from banana peel |
| title_short | Extraction conditions of polyphenol oxidase from banana peel |
| title_sort | extraction conditions of polyphenol oxidase from banana peel |
| topic | "Polyphenol oxidase; Banana peel; Extraction" |
| url | http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/2292 |
| work_keys_str_mv | AT baghdadsciencejournal extractionconditionsofpolyphenoloxidasefrombananapeel |