<i>Angelicae Pubescentis</i> Radix Remitted Intestine Damage in Mice Induced by <i>Escherichia coli</i> via Mediating Antioxidant Defense, Inflammatory Mediators, and Restoring Gut Microbiota
This study aims to explore the therapeutic potential of <i>Angelicae Pubescentis</i> Radix (APR), a traditional Chinese medicine that is widely known for its anti-inflammatory, anti-oxidative, and anti-microbial properties, using a mouse model. In this study, 30 mice were selected and di...
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MDPI AG
2025-04-01
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| author | Kehong Deng Chang Xu Qing He Muhammad Safdar Mudassar Nazar Xiaocong Li Kun Li |
| author_facet | Kehong Deng Chang Xu Qing He Muhammad Safdar Mudassar Nazar Xiaocong Li Kun Li |
| author_sort | Kehong Deng |
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| description | This study aims to explore the therapeutic potential of <i>Angelicae Pubescentis</i> Radix (APR), a traditional Chinese medicine that is widely known for its anti-inflammatory, anti-oxidative, and anti-microbial properties, using a mouse model. In this study, 30 mice were selected and divided into three groups: control group (CD), infection group (ED), and treatment group (TD). Mice in the TD were gavaged with APR oil (0.15 mL/kg/day) for 20 days, while mice in the CD and ED received an equal volume of normal saline. On the 21st day, mice in the ED and TD were infected with multi-drug-resistant <i>E. coli</i> (1 × 10<sup>7</sup> CFU/mL) derived from diarrheal yak. Twenty-four hours later, all mice were euthanized, and blood, organs, and intestinal samples were collected for analysis. The results of intestinal sections and intestinal bacterial load revealed that APR treatment significantly reduced (<i>p</i> < 0.05) both bacterial load and intestinal injury. Serum analysis indicated that APR treatment also alleviated the inflammation and oxidative stress induced by <i>E. coli</i> infection. Intestinal microbiota sequencing further showed that APR treatment increased the abundance of intestinal probiotics such as <i>Ligilactobacillus</i>, <i>Paludicola</i>, and <i>Blautia_A_1417806</i> while also enhancing the enrichment of functional pathways associated with antioxidant defense. In conclusion, APR treatment effectively alleviates diseases caused by <i>E. coli</i> infection, promotes the growth of beneficial intestinal bacteria, and improves the antioxidant capacity in animals. Additionally, these findings confirm APR’s role in addressing immediate effects rather than chronic adaptations. Future studies should investigate the prolonged effects of APR treatment beyond the acute phase. |
| format | Article |
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| institution | OA Journals |
| issn | 2306-7381 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
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| series | Veterinary Sciences |
| spelling | doaj-art-e9e840840cc9427f8a27c05760add0b62025-08-20T02:25:07ZengMDPI AGVeterinary Sciences2306-73812025-04-0112435410.3390/vetsci12040354<i>Angelicae Pubescentis</i> Radix Remitted Intestine Damage in Mice Induced by <i>Escherichia coli</i> via Mediating Antioxidant Defense, Inflammatory Mediators, and Restoring Gut MicrobiotaKehong Deng0Chang Xu1Qing He2Muhammad Safdar3Mudassar Nazar4Xiaocong Li5Kun Li6Department of Pharmacy, Hubei Three Gorges Vocational and Technical College, Yichang 443000, ChinaCollege of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Breeding and Genetics, Cholistan University of Veterinary and Animal Sciences, Bahawalpur 63100, PakistanDepartment of Clinical Medicine and Surgery, Faculty of Veterinary Sciences, Constituent College Burewala, University of Agriculture Faisalabad, Burewala 61010, PakistanDepartment of Pharmacy, Hubei Three Gorges Vocational and Technical College, Yichang 443000, ChinaCollege of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, ChinaThis study aims to explore the therapeutic potential of <i>Angelicae Pubescentis</i> Radix (APR), a traditional Chinese medicine that is widely known for its anti-inflammatory, anti-oxidative, and anti-microbial properties, using a mouse model. In this study, 30 mice were selected and divided into three groups: control group (CD), infection group (ED), and treatment group (TD). Mice in the TD were gavaged with APR oil (0.15 mL/kg/day) for 20 days, while mice in the CD and ED received an equal volume of normal saline. On the 21st day, mice in the ED and TD were infected with multi-drug-resistant <i>E. coli</i> (1 × 10<sup>7</sup> CFU/mL) derived from diarrheal yak. Twenty-four hours later, all mice were euthanized, and blood, organs, and intestinal samples were collected for analysis. The results of intestinal sections and intestinal bacterial load revealed that APR treatment significantly reduced (<i>p</i> < 0.05) both bacterial load and intestinal injury. Serum analysis indicated that APR treatment also alleviated the inflammation and oxidative stress induced by <i>E. coli</i> infection. Intestinal microbiota sequencing further showed that APR treatment increased the abundance of intestinal probiotics such as <i>Ligilactobacillus</i>, <i>Paludicola</i>, and <i>Blautia_A_1417806</i> while also enhancing the enrichment of functional pathways associated with antioxidant defense. In conclusion, APR treatment effectively alleviates diseases caused by <i>E. coli</i> infection, promotes the growth of beneficial intestinal bacteria, and improves the antioxidant capacity in animals. Additionally, these findings confirm APR’s role in addressing immediate effects rather than chronic adaptations. Future studies should investigate the prolonged effects of APR treatment beyond the acute phase.https://www.mdpi.com/2306-7381/12/4/354<i>Angelicae Pubescentis</i> Radixbacterial diarrheaintestinal micro-ecosystemantioxidant defensegut microbiota |
| spellingShingle | Kehong Deng Chang Xu Qing He Muhammad Safdar Mudassar Nazar Xiaocong Li Kun Li <i>Angelicae Pubescentis</i> Radix Remitted Intestine Damage in Mice Induced by <i>Escherichia coli</i> via Mediating Antioxidant Defense, Inflammatory Mediators, and Restoring Gut Microbiota Veterinary Sciences <i>Angelicae Pubescentis</i> Radix bacterial diarrhea intestinal micro-ecosystem antioxidant defense gut microbiota |
| title | <i>Angelicae Pubescentis</i> Radix Remitted Intestine Damage in Mice Induced by <i>Escherichia coli</i> via Mediating Antioxidant Defense, Inflammatory Mediators, and Restoring Gut Microbiota |
| title_full | <i>Angelicae Pubescentis</i> Radix Remitted Intestine Damage in Mice Induced by <i>Escherichia coli</i> via Mediating Antioxidant Defense, Inflammatory Mediators, and Restoring Gut Microbiota |
| title_fullStr | <i>Angelicae Pubescentis</i> Radix Remitted Intestine Damage in Mice Induced by <i>Escherichia coli</i> via Mediating Antioxidant Defense, Inflammatory Mediators, and Restoring Gut Microbiota |
| title_full_unstemmed | <i>Angelicae Pubescentis</i> Radix Remitted Intestine Damage in Mice Induced by <i>Escherichia coli</i> via Mediating Antioxidant Defense, Inflammatory Mediators, and Restoring Gut Microbiota |
| title_short | <i>Angelicae Pubescentis</i> Radix Remitted Intestine Damage in Mice Induced by <i>Escherichia coli</i> via Mediating Antioxidant Defense, Inflammatory Mediators, and Restoring Gut Microbiota |
| title_sort | i angelicae pubescentis i radix remitted intestine damage in mice induced by i escherichia coli i via mediating antioxidant defense inflammatory mediators and restoring gut microbiota |
| topic | <i>Angelicae Pubescentis</i> Radix bacterial diarrhea intestinal micro-ecosystem antioxidant defense gut microbiota |
| url | https://www.mdpi.com/2306-7381/12/4/354 |
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