Mutagenic biomarkers in erythrocytes of free-ranging and captive Kinosternon scorpioides from the Brazilian Amazon
This study aimed to investigate the occurrence of micronuclei (MN) and erythrocyte nuclear abnormalities (ENAs) in natural and captive Kinosternon scorpioides populations and compare their frequencies with the environment type, sex, and seasonal period in an environmental protection area in the sta...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Goiás
2025-07-01
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| Series: | Ciência Animal Brasileira |
| Online Access: | https://revistas.ufg.br/vet/article/view/81855 |
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| author | Carlos Alailson Licar Rodrigues Almerinda Macieira Medeiros Elizângela Pinheiro Pereira Katherine Saldanha Noleto Raimunda Nonata Fortes Carvalho Neta Germán Augusto Murrieta Morey Alana Lislea de Sousa |
| author_facet | Carlos Alailson Licar Rodrigues Almerinda Macieira Medeiros Elizângela Pinheiro Pereira Katherine Saldanha Noleto Raimunda Nonata Fortes Carvalho Neta Germán Augusto Murrieta Morey Alana Lislea de Sousa |
| author_sort | Carlos Alailson Licar Rodrigues |
| collection | DOAJ |
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This study aimed to investigate the occurrence of micronuclei (MN) and erythrocyte nuclear abnormalities (ENAs) in natural and captive Kinosternon scorpioides populations and compare their frequencies with the environment type, sex, and seasonal period in an environmental protection area in the state of Maranhão, Amazon. Blood was collected from 112 specimens from regions with direct anthropogenic influence and indirect and control areas to produce blood smears for subsequent cytological analysis under an optical microscope. We found MN (N=91) and ENAs such as binucleated nuclei (N=14), notched (N=81), lobulated (N=35), segmented (N=16), and displaced (N=253) in animals in situ. No abnormalities were recorded in the erythrocytes of the captive specimens. The mean (±standard deviation) MN (1.13±0.47) differed from those of the other chelonians from the natural environment, and the displaced and binucleated nuclei manifested themselves at higher (51.6%) and lower (2.8%) frequencies, respectively. Lobulated nuclei frequency was significantly different between the areas (p<0.05); segmented nuclei (p<0.05) and displaced (p<0.05) had higher frequencies during the dry and rainy periods. Sex did not affect these abnormalities. No significant MN were found; however, erythrocytes with lobulated, segmented, and displaced nuclei were documented in animals from different environments and seasonal periods. Our study provides pioneering data on MN and nuclear anomalies in the erythrocytes of K. scorpioides and confirms the significant differences and relationships between them. The occurrence of abnormalities in wild animals demonstrates the need to maintain protected areas and the continuity of conservation actions aimed at Amazonian turtles. This contributes to reducing the impact on conservation units that employ the sustainable use of biodiversity in Brazil.
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| format | Article |
| id | doaj-art-e9d9948242744689a4bb8ef8e3499770 |
| institution | Kabale University |
| issn | 1518-2797 1809-6891 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Universidade Federal de Goiás |
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| series | Ciência Animal Brasileira |
| spelling | doaj-art-e9d9948242744689a4bb8ef8e34997702025-08-20T03:31:31ZengUniversidade Federal de GoiásCiência Animal Brasileira1518-27971809-68912025-07-0126Mutagenic biomarkers in erythrocytes of free-ranging and captive Kinosternon scorpioides from the Brazilian AmazonCarlos Alailson Licar Rodrigues0Almerinda Macieira Medeiros1Elizângela Pinheiro Pereira2Katherine Saldanha Noleto3Raimunda Nonata Fortes Carvalho Neta4Germán Augusto Murrieta Morey5Alana Lislea de Sousa6Universidade Estadual do Maranhão (UEMA), São Luís, Maranhão, Brazil Instituto Federal do Maranhão (IFMA), Barreirinhas, Maranhão, Brazil Universidade Estadual do Maranhão (UEMA), São Luís, Maranhão, Brazil Universidade Federal do Maranhão (UFMA), São Luís, Maranhão, Brazil Universidade Federal do Maranhão (UFMA), São Luís, Maranhão, Brazil Instituto de Investigaciones de la Amazonía Peruana (IIAP), San Juan Bautista, Iquitos, Loreto-PeruUniversidade Estadual do Maranhão (UEMA), São Luís, Maranhão, Brazil This study aimed to investigate the occurrence of micronuclei (MN) and erythrocyte nuclear abnormalities (ENAs) in natural and captive Kinosternon scorpioides populations and compare their frequencies with the environment type, sex, and seasonal period in an environmental protection area in the state of Maranhão, Amazon. Blood was collected from 112 specimens from regions with direct anthropogenic influence and indirect and control areas to produce blood smears for subsequent cytological analysis under an optical microscope. We found MN (N=91) and ENAs such as binucleated nuclei (N=14), notched (N=81), lobulated (N=35), segmented (N=16), and displaced (N=253) in animals in situ. No abnormalities were recorded in the erythrocytes of the captive specimens. The mean (±standard deviation) MN (1.13±0.47) differed from those of the other chelonians from the natural environment, and the displaced and binucleated nuclei manifested themselves at higher (51.6%) and lower (2.8%) frequencies, respectively. Lobulated nuclei frequency was significantly different between the areas (p<0.05); segmented nuclei (p<0.05) and displaced (p<0.05) had higher frequencies during the dry and rainy periods. Sex did not affect these abnormalities. No significant MN were found; however, erythrocytes with lobulated, segmented, and displaced nuclei were documented in animals from different environments and seasonal periods. Our study provides pioneering data on MN and nuclear anomalies in the erythrocytes of K. scorpioides and confirms the significant differences and relationships between them. The occurrence of abnormalities in wild animals demonstrates the need to maintain protected areas and the continuity of conservation actions aimed at Amazonian turtles. This contributes to reducing the impact on conservation units that employ the sustainable use of biodiversity in Brazil. https://revistas.ufg.br/vet/article/view/81855 |
| spellingShingle | Carlos Alailson Licar Rodrigues Almerinda Macieira Medeiros Elizângela Pinheiro Pereira Katherine Saldanha Noleto Raimunda Nonata Fortes Carvalho Neta Germán Augusto Murrieta Morey Alana Lislea de Sousa Mutagenic biomarkers in erythrocytes of free-ranging and captive Kinosternon scorpioides from the Brazilian Amazon Ciência Animal Brasileira |
| title | Mutagenic biomarkers in erythrocytes of free-ranging and captive Kinosternon scorpioides from the Brazilian Amazon |
| title_full | Mutagenic biomarkers in erythrocytes of free-ranging and captive Kinosternon scorpioides from the Brazilian Amazon |
| title_fullStr | Mutagenic biomarkers in erythrocytes of free-ranging and captive Kinosternon scorpioides from the Brazilian Amazon |
| title_full_unstemmed | Mutagenic biomarkers in erythrocytes of free-ranging and captive Kinosternon scorpioides from the Brazilian Amazon |
| title_short | Mutagenic biomarkers in erythrocytes of free-ranging and captive Kinosternon scorpioides from the Brazilian Amazon |
| title_sort | mutagenic biomarkers in erythrocytes of free ranging and captive kinosternon scorpioides from the brazilian amazon |
| url | https://revistas.ufg.br/vet/article/view/81855 |
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