Biocontrol Activity of New Lactic Acid Bacteria Isolates Against Fusaria and <i>Fusarium</i> Mycotoxins
As significant fungal pathogens of crops, Fusaria species contaminate various food and feed commodities. Some of the <i>Fusarium</i> spp. secondary metabolites (e.g., trichothecenes, zearalenone, and fumonisins) are widely known toxic molecules (mycotoxins) with chronic and acute effects...
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MDPI AG
2025-02-01
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| Series: | Toxins |
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| author | S. Vipin Krishnan P. A. Anaswara K. Madhavan Nampoothiri Szilvia Kovács Cintia Adácsi Pál Szarvas Szabina Király István Pócsi Tünde Pusztahelyi |
| author_facet | S. Vipin Krishnan P. A. Anaswara K. Madhavan Nampoothiri Szilvia Kovács Cintia Adácsi Pál Szarvas Szabina Király István Pócsi Tünde Pusztahelyi |
| author_sort | S. Vipin Krishnan |
| collection | DOAJ |
| description | As significant fungal pathogens of crops, Fusaria species contaminate various food and feed commodities. Some of the <i>Fusarium</i> spp. secondary metabolites (e.g., trichothecenes, zearalenone, and fumonisins) are widely known toxic molecules (mycotoxins) with chronic and acute effects on humans and animals. The growing demand for safer, pesticide-free food drives us to increase biological control during crop growing. Recent research suggests that lactic acid bacteria (LABs) as biocontrol are the best choice for extenuating <i>Fusarium</i> mycotoxins. Newly isolated LABs were tested as antifungal agents against <i>Fusarium verticillioides</i>, <i>F. graminearum</i>, and <i>F. oxysporum</i>. The characterized and genetically identified LABs belonged to <i>Limosilactobacillus fermentum</i> (SD4) and <i>Lactiplantibacillus plantarum</i> (FCW4 and CB2) species. All tested LABs and their cell-free culture supernatants showed antagonism on the MRS solid medium. The antifungal activity was also demonstrated on surface-sterilized wheat and peanuts. The germination test of corn kernels proved that the LAB strains SD4 and FCW4 significantly (<i>p</i> < 0.05) enhanced root and shoot development in plantlets while simultaneously suppressing the outgrowth of <i>F. verticillioides</i>. Small-scale corn silage fermentation revealed the significant effects of SD4 supplementation (decreased zearalenone, lower mold count, and total reduction of deoxynivalenol) within the mixed populations. |
| format | Article |
| id | doaj-art-e9d95dac6ddd4ca7866e9836b246ec12 |
| institution | OA Journals |
| issn | 2072-6651 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
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| series | Toxins |
| spelling | doaj-art-e9d95dac6ddd4ca7866e9836b246ec122025-08-20T02:04:05ZengMDPI AGToxins2072-66512025-02-011726810.3390/toxins17020068Biocontrol Activity of New Lactic Acid Bacteria Isolates Against Fusaria and <i>Fusarium</i> MycotoxinsS. Vipin Krishnan0P. A. Anaswara1K. Madhavan Nampoothiri2Szilvia Kovács3Cintia Adácsi4Pál Szarvas5Szabina Király6István Pócsi7Tünde Pusztahelyi8Microbial Processes and Technology Division (MPTD), CSIR-National Institute for Interdisciplinary Science and Technology (NIIST), Thiruvananthapuram 695019, IndiaMicrobial Processes and Technology Division (MPTD), CSIR-National Institute for Interdisciplinary Science and Technology (NIIST), Thiruvananthapuram 695019, IndiaMicrobial Processes and Technology Division (MPTD), CSIR-National Institute for Interdisciplinary Science and Technology (NIIST), Thiruvananthapuram 695019, IndiaFood and Environmental Toxicology Research Group, Central Laboratory of Agricultural and Food Products, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, H-4032 Debrecen, HungaryFood and Environmental Toxicology Research Group, Central Laboratory of Agricultural and Food Products, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, H-4032 Debrecen, HungaryCentre for Agricultural Genomics and Biotechnology, Faculty of Agricultural and Food Sciences and Environmental Management Center, University of Debrecen, H-4400 Nyíregyháza, HungaryDepartment of Molecular Biotechnology and Microbiology, Institute of Biotechnology, Faculty of Science and Technology, University of Debrecen, H-4032 Debrecen, HungaryDepartment of Molecular Biotechnology and Microbiology, Institute of Biotechnology, Faculty of Science and Technology, University of Debrecen, H-4032 Debrecen, HungaryFood and Environmental Toxicology Research Group, Central Laboratory of Agricultural and Food Products, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, H-4032 Debrecen, HungaryAs significant fungal pathogens of crops, Fusaria species contaminate various food and feed commodities. Some of the <i>Fusarium</i> spp. secondary metabolites (e.g., trichothecenes, zearalenone, and fumonisins) are widely known toxic molecules (mycotoxins) with chronic and acute effects on humans and animals. The growing demand for safer, pesticide-free food drives us to increase biological control during crop growing. Recent research suggests that lactic acid bacteria (LABs) as biocontrol are the best choice for extenuating <i>Fusarium</i> mycotoxins. Newly isolated LABs were tested as antifungal agents against <i>Fusarium verticillioides</i>, <i>F. graminearum</i>, and <i>F. oxysporum</i>. The characterized and genetically identified LABs belonged to <i>Limosilactobacillus fermentum</i> (SD4) and <i>Lactiplantibacillus plantarum</i> (FCW4 and CB2) species. All tested LABs and their cell-free culture supernatants showed antagonism on the MRS solid medium. The antifungal activity was also demonstrated on surface-sterilized wheat and peanuts. The germination test of corn kernels proved that the LAB strains SD4 and FCW4 significantly (<i>p</i> < 0.05) enhanced root and shoot development in plantlets while simultaneously suppressing the outgrowth of <i>F. verticillioides</i>. Small-scale corn silage fermentation revealed the significant effects of SD4 supplementation (decreased zearalenone, lower mold count, and total reduction of deoxynivalenol) within the mixed populations.https://www.mdpi.com/2072-6651/17/2/68lactic acid bacterium<i>Fusarium</i>antagonismmycotoxincornwheat |
| spellingShingle | S. Vipin Krishnan P. A. Anaswara K. Madhavan Nampoothiri Szilvia Kovács Cintia Adácsi Pál Szarvas Szabina Király István Pócsi Tünde Pusztahelyi Biocontrol Activity of New Lactic Acid Bacteria Isolates Against Fusaria and <i>Fusarium</i> Mycotoxins Toxins lactic acid bacterium <i>Fusarium</i> antagonism mycotoxin corn wheat |
| title | Biocontrol Activity of New Lactic Acid Bacteria Isolates Against Fusaria and <i>Fusarium</i> Mycotoxins |
| title_full | Biocontrol Activity of New Lactic Acid Bacteria Isolates Against Fusaria and <i>Fusarium</i> Mycotoxins |
| title_fullStr | Biocontrol Activity of New Lactic Acid Bacteria Isolates Against Fusaria and <i>Fusarium</i> Mycotoxins |
| title_full_unstemmed | Biocontrol Activity of New Lactic Acid Bacteria Isolates Against Fusaria and <i>Fusarium</i> Mycotoxins |
| title_short | Biocontrol Activity of New Lactic Acid Bacteria Isolates Against Fusaria and <i>Fusarium</i> Mycotoxins |
| title_sort | biocontrol activity of new lactic acid bacteria isolates against fusaria and i fusarium i mycotoxins |
| topic | lactic acid bacterium <i>Fusarium</i> antagonism mycotoxin corn wheat |
| url | https://www.mdpi.com/2072-6651/17/2/68 |
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